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Lactobacillus culture medium, method for preparing folic acid and method for determining folic acid content

A culture medium and a technology for lactic acid bacteria, which are applied in the preparation of folic acid, determine the content of folic acid in fermentation broth, and determine the content of folic acid in lactic acid bacteria culture medium, can solve problems to be improved, etc., and achieve the effects of high safety, high content and good absorption effect

Inactive Publication Date: 2015-11-18
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current lactic acid bacteria culture medium and the method for preparing folic acid still need to be improved

Method used

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  • Lactobacillus culture medium, method for preparing folic acid and method for determining folic acid content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In this example, folic acid was prepared by the following steps:

[0050] (1) Preparation of seed medium: weigh 5g of peptone, 5g of beef extract, 2g of yeast extract, 10g of glucose, K 2 HPO 4 1g, CH 3 COONa2g, Tween-800.5g, diamine citrate 1g, MgSO 4 ·7H 2 O0.2g, MnSO 4 4H 2 O0.1g, adjust the pH value to 7 with deionized water, and dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0051] (2) Preparation of lactic acid bacteria medium: weigh 5g of peptone, 5g of beef extract, 2g of yeast extract, 10g of glucose, K 2 HPO 4 1g, CH 3 COONa2g, Tween-800.5g, diamine citrate 1g, MgSO 4 ·7H 2 O0.2g, MnSO 4 4H 2 O0.1g, p-aminobenzoic acid 100g, glutamic acid 1g, adjust the pH value to 5.5, dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0052] (3) Lactobacillus delbrueckii subsp. bulgaricus 20089 was inoculated in the seed liquid medium at an inoculum amount of 3%, activated and cultured at ...

Embodiment 2

[0057] In this example, folic acid was prepared by the following steps:

[0058] (1) Preparation of seed medium: Weigh 15g of peptone, 15g of beef extract, 10g of yeast extract, 30g of glucose, K 2 HPO 4 5g, CH 3 COONa10g, Tween-802g, diamine citrate 5g, MgSO 4 ·7H 2 O1g, MnSO 4 4H 2 O0.5g, adjust the pH value to 7, dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0059] (2) Preparation of lactic acid bacteria medium: weigh 15g of peptone, 15g of beef extract, 10g of yeast extract, 30g of glucose, K 2 HPO 4 5g, CH 3 COONa10g, Tween-802g, diamine citrate 5g, MgSO 4 ·7H 2 O1g, MnSO 4 4H 2 O0.5g, p-aminobenzoic acid 1000g, glutamic acid 5g, adjust the pH value to 7.5, dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0060] (3) Lactobacillus delbrueckii subsp. bulgaricus 20089 was inoculated in the seed liquid medium at an inoculum amount of 3%, activated and cultured at 42 degrees Celsius for 2...

Embodiment 3

[0065] In this example, folic acid was prepared by the following steps:

[0066] (1) Preparation of seed medium: Weigh 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, K 2 HPO 4 2g, CH 3 COONa5g, Tween-801g, diamine citrate 2g, MgSO 4 ·7H 2 O0.5g, MnSO 4 4H 2 O0.3g, adjust the pH value to 7, dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0067] (2) Preparation of lactic acid bacteria medium: Weigh 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, K 2 HPO 4 2g, CH 3 COONa5g, Tween-801g, diamine citrate 2g, MgSO 4 ·7H 2 O0.5g, MnSO 4 4H 2 O0.3g, p-aminobenzoic acid 400g, glutamic acid 4g, adjust the pH value to 7, dilute to 1L with deionized water; sterilize at 121 degrees Celsius for 15 minutes.

[0068] (3) Lactobacillus delbrueckii subsp. bulgaricus 20089 was inoculated in the seed liquid medium at an inoculum amount of 3%, activated and cultured at 42 degrees Celsius for 24 h...

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Abstract

The invention provides a lactobacillus culture medium, a method for preparing folic acid and a method for determining folic acid content in a fermentation liquid. According to the embodiment of the invention, every 1 liter of lactobacillus culture medium comprises 10g of glutamic acid, 10g of beef extract, 5g of yeast extract, 20g of glucose, 2g of K2HPO4, 5g of CH3COONa, 1g of Tween-80, 2g of diamine citrate, 0.5g of MgSO4.7H2O, 0.3g of MnSO4.4H2O, 400g of p-aminobenzoic acid and 4g of glutamic acid. The lactobacillus culture medium at least has one of the following advantages: high content, high safety, favorable absorption effect, and no toxic or side effect.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to a culture medium for lactic acid bacteria, a method for preparing folic acid and a method for determining the content of folic acid. More specifically, the present invention relates to a culture medium for lactic acid bacteria, a method for preparing folic acid and a method for determining the content of folic acid in a fermentation broth. Background technique [0002] Folic acid is composed of pteridine, p-aminobenzoic acid and L-glutamic acid, also called pteroyl glutamic acid, which is a kind of B vitamins. The human body cannot synthesize folic acid on its own, and is almost entirely dependent on external intake. When the intake is insufficient or the utilization rate is reduced, symptoms of folic acid deficiency will appear in the body. Natural folic acid widely exists in animal and plant foods, but because natural folic acid is extremely unstab...

Claims

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Application Information

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IPC IPC(8): C12P17/18G01N30/02C12R1/225
Inventor 杨岚陈云史玉东李周勇赵晓慧
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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