Chloramphenicol molecular imprinting electrochemical luminescence sensor and chloramphenicol detection method thereof

A technique of molecular imprinting and chloramphenicol

Inactive Publication Date: 2015-11-18
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The embodiment of the invention discloses a chloramphenicol molecularly imprinted electrochemiluminescence sensor and a method for dete...

Method used

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  • Chloramphenicol molecular imprinting electrochemical luminescence sensor and chloramphenicol detection method thereof
  • Chloramphenicol molecular imprinting electrochemical luminescence sensor and chloramphenicol detection method thereof
  • Chloramphenicol molecular imprinting electrochemical luminescence sensor and chloramphenicol detection method thereof

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Embodiment 1

[0069] 1.1 Synthesis of poly-o-phenylenediamine-chloramphenicol film

[0070] First, the gold electrode was polished with 0.05 μm alumina powder, and the treated gold electrode was ultrasonically oscillated in ethanol and distilled water for 5 min, rinsed with distilled water, and dried for later use.

[0071] 0.0646g chloramphenicol and 0.0316g o-phenylenediamine are dissolved in 20mLpH and be that the amount concentration of 5.2 substance is in the acetic acid buffer solution of 0.1mol / L, obtain chloramphenicol and o-phenylenediamine maximum concentration and be 10mmol / L respectively, The acetic acid buffer solution is used as the electrolyte, the Ag / AgCl electrode is used as the reference electrode, the platinum electrode is used as the counter electrode, and the gold electrode is used as the working electrode. Cyclic voltammetry is used for scanning, the voltage range is 0-0.8V, and the scanning speed is 0.05V. / s, and the number of scanning circles was 30 circles, and a p...

Embodiment 2

[0080] 0.323g chloramphenicol and 0.079g o-phenylenediamine are dissolved in the acetic acid buffer solution that 20mLpH is 5.2 and the amount concentration of substance is 0.05mol / L, and the final concentration that obtains chloramphenicol is 50mmol / L, and the final concentration of o-phenylenediamine 25mmol / L, using the acetic acid buffer solution as the electrolyte, the Ag / AgCl electrode as the reference electrode, the platinum electrode as the counter electrode, and the graphite electrode as the working electrode, using cyclic voltammetry to scan, the voltage range is 0-0.8V, The scanning speed was 0.05V / s, and the number of scanning circles was 40, and a poly-o-phenylenediamine-chloramphenicol film was obtained on the surface of the gold electrode.

[0081] Rinse the poly-o-phenylenediamine-chloramphenicol film on the surface of the gold electrode with 0.01mol / LNaOH solution, rinse for 30min, and wash away the chloramphenicol in the poly-o-phenylenediamine-chloramphenicol ...

Embodiment 3

[0083] 0.162g chloramphenicol and 0.158g o-phenylenediamine are dissolved in 20mLpH and be 5.2 substance concentration in the acetic acid buffer solution of 0.2mol / L, obtain chloramphenicol final concentration and be 25mmol / L, o-phenylenediamine final concentration 50mmol / L, using acetic acid buffer solution as electrolyte, Ag / AgCl electrode as reference electrode, platinum electrode as counter electrode, and platinum electrode as working electrode, using cyclic voltammetry to scan, the voltage range is 0-0.8V, The scanning speed was 0.05V / s, and the number of scanning circles was 20, and a poly-o-phenylenediamine-chloramphenicol film was obtained on the surface of the gold electrode.

[0084] Rinse the poly-o-phenylenediamine-chloramphenicol film on the surface of the gold electrode with 0.01mol / L HCl solution, rinse for 15min, and wash away the chloramphenicol in the poly-o-phenylenediamine-chloramphenicol film, and obtain the chloramphenicol Molecularly imprinted electroche...

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Abstract

Embodiments of the present invention disclose a chloramphenicol molecular imprinting electrochemical luminescence sensor, which comprises an inert electrode and a chloramphenicol molecular imprinting polymer film on the inert electrode surface. The chloramphenicol molecular imprinting polymer film preparation steps comprise: adopting an acetic acid buffer solution containing chloramphenicol and o-phenylene diamine as an electrolyte, and adopting an inert electrode as a working electrode to construct a three-electrode system; using a cyclic voltammetry method to obtain a poly o-phenylene diamine-chloramphenicol film, wherein the polymerization potential is 0-0.8 V; and carrying out elution with an eluant to obtain the chloramphenicol molecular imprinting polymer film, wherein the pH value of the acetic acid buffer solution is 5.2, the molar concentration of the acetic acid buffer solution is 0.05-0.2 mol/L, and the molar concentrations of the chloramphenicol and the o-phenylene diamine respectively are 1-50 mmol/L. According to the present invention, the chloramphenicol molecular imprinting electrochemical luminescence sensor can be used for chloramphenicol detection, and has characteristics of high detection sensitivity and good selectivity.

Description

technical field [0001] The invention relates to the field of antibiotic detection, in particular to a chloramphenicol molecularly imprinted electrochemiluminescent sensor and a method for detecting chloramphenicol. Background technique [0002] Chloramphenicol is a bacteriostatic broad-spectrum antibiotic produced by Streptomyces venezuela, which can effectively kill or inhibit various Gram-positive bacteria and negative bacteria. In recent years, chloramphenicol has been widely used in the treatment of human and animal infectious diseases, and is often added to feed to promote the growth and development of animals, resulting in chloramphenicol residues in food. Long-term use of chloramphenicol-containing medicines or foods containing chloramphenicol will have very toxic side effects on the human body, and even cause serious diseases, such as gray baby syndrome, leukemia and aplastic anemia. Therefore, detecting the content of chloramphenicol in food is of great significanc...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/26
Inventor 刘红云廉文静
Owner BEIJING NORMAL UNIVERSITY
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