Fluorescence-labeled azo-modified nucleotide and application thereof in DNA sequencing

A fluorescent labeling and nucleotide technology, applied in the field of DNA sequencing, can solve the problems of insufficient shearing conditions, low efficiency, short read length, etc.
CN105085591AActive Publication Date: 2015-11-25SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2015-11-25

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Abstract

The invention discloses a fluorescence-labeled azo-modified nucleotide and application thereof in DNA sequencing. The nucleotide has a structural formula as shown in a formula I, wherein R1, R2, R3, R4, R5 and R6 may not synchronously be H, and m and n are integers of 0 to 10. Compared with the prior art, novel reversible terminators based on azo connection units are synthesized; a denaturing PAGE gel, i.e. a sequencing gel proves that the reversible terminators can only extend a reversible terminator at a time and has extension efficiency of 100% when a template is a plurality of continuous same basic groups; thus, under the action of a reducing agent, highly-efficient, rapid and full shearing can be realized, and great potential and value applied in the DNA sequencing are obtained; meanwhile, raw materials needed by synthesis of the azo-modified nucleotide are simple and easily available, and synthetic process is a conventional chemical reaction and can be used for popularization and application.
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Description

technical field

[0001] The invention belongs to the technical field of DNA sequencing, and in particular relates to a fluorescently labeled azo-modified nucleotide and its application in DNA sequencing. Background technique

[0002] DNA sequencing technology is one of the important means in modern biological research. After the completion of the Human Genome Project, DNA sequencing technology has developed rapidly. DNA sequencing (DNA sequencing) refers to the analysis of the base sequence of a specific DNA fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). The development of accurate, high-throughput, and low-cost DNA sequencing methods is of great significance to biological and medical sciences.

[0003] Sequencing by synthesis (Sequencing By Synthesis, SBS) is one of the next-generation DNA sequencing technologies. The sequencing by synthesis method immobilizes a large number of template DNA fragments to be tested, hybridizes ...

Claims

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