Preparation method for G-SERS (Graphene surface enhanced Raman spectra) substrate and cancer cell detection method
A technology of cancer cells and liver cancer cells, applied in the field of integrated optics and medicine, can solve the problems of limited signal enhancement and limited practical application, and achieve the effect of increasing the probability of action, high test efficiency and improving efficiency
Inactive Publication Date: 2015-11-25
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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Although surface-enhanced Raman spectroscopy can improve the sensitivity of graphene Raman signals, the signal enhancement of pure biomass / cancer cells is limited, which also limits its practical application.
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[0036] The present invention will be further described below in conjunction with the accompanying drawings.
[0037] Such as Figure 7 As shown, it is a silver nanostructure-graphene-gold nanostructure device, and its preparation method is as follows:
[0038] Step 1: Preparation of gold nanostructures.
[0039] By means of electron beam evaporation of a coating machine (SyskeyTechnology), under the condition of ultra-low vacuum (less than 8e-7torr), high-purity gold (99.999%) is evaporated on a silicon substrate (Si, ~8mmX10mm), and the evaporation rate is 0.5 -5 Angstroms / second, forming a gold film with a thickness of 1-10 nm, thereby forming different gold nanostructures (nanoparticles, nanobars) on the silicon substrate.
[0040] Step 2: Preparation of graphene-gold nanostructure substrate.
[0041]Method 1: Dissolve graphene oxide in deionized water to form a solution of about 4-10 mg / ml, and form the solution on the substrate of step 1 above by using a homogenizer (K...
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Abstract
The invention provides a preparation method for G-SERS (Graphene surface enhanced Raman spectra) substrate and a cancer cell detection method. The preparation method for the G-SERS (Graphene surface enhanced Raman spectra) substrate comprises the following steps: step 1, preparing a gold nanometer structure; step 2, preparing a graphene-gold nanometer structure substrate; and step 3, preparing a silver nanometer structure-graphene-gold nanometer structure device. The cancer cell detection method is a simple universal rapid low-cost method for realizing detection on a cancer cell by utilizing a surface enhanced Raman principle. According to the preparation method, the dimension of the prepared micro-nano structural device can be small up to micrometer. The testing efficiency is high, surface Raman is enhanced by utilizing a metal micro-nano structure, the acting probability of incident light on a substance is increased, the structural information of the cancer cell can be relatively sensitively expressed, and the detection and diagnosis efficiencies are improved. The cancer cell does not need pretreatment and marking, the difficulty of marking the cancer cell in advance in the prior art is overcome, and the accuracy and the reliability of detection and diagnosis are improved at the same time.
Description
technical field [0001] The invention relates to integrated optics and medicine, in particular to a preparation method of a silver nanostructure-graphene-gold nanostructure device and a cancer cell detection method. Background technique [0002] Cancer is a major problem in medicine. From the discovery of cancer to diagnosis, it not only wastes a lot of time but also costs a lot of money. These problems have plagued cancer patients and medical workers for many years. It is necessary to develop a reliable, simple, effective, and low-cost means to detect and diagnose cancer cells and normal cells. Raman spectroscopy is a technology developed by using the inelastic scattering effect of optics. After years of development, surface Raman optical enhancement has been widely studied and can be used in biological detection, imaging, molecular selection and other fields. However, current research shows that the detection and diagnosis of cancer cells requires labeling substances to ma...
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Login to View More IPC IPC(8): G01N21/65
Inventor 肖淑敏易宁波宋清海
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL