Method for utilizing sensitive silkworm embryos to detect genetic toxic substances in environment

Abstract

The invention discloses a method for utilizing sensitive silkworm embryos to detect genetic toxic substances in an environment. The method sequentially comprises the following steps that 1, active silkworm eggs are selected; 2, the sensitive silkworm embryos, namely end-reversal-stage silkworm eggs marked as F2, are obtained; 3, the sensitive silkworm embryos are arranged in different genetic toxic substances to be subjected to exposure treatment; 4, the silkworm eggs subjected to exposure treatment are incubated, the incubating rate is examined after incubation, and the genetic toxic substances corresponding to the silkworms having high incubating rate are relatively lower in toxicity. The method is mainly used for utilizing the sensitive silkworm embryos to detect the genetic toxic substances in the environment, and the approximate concentration or dosage of the genetic toxic substances can be calculated by means of the method within a certain dosage range.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for detecting genetic poisons in the environment by using the embryos of environmentally sensitive silkworms. Background technique [0002] With the development of industrial production, especially township enterprises, the rural environment is deteriorating, and more and more genotoxic substances (genotoxic substances, physical or chemical factors that cause damage to the DNA of organism cells) such as heavy metals, ion radiation, pesticides ( Insecticides, herbicides), aldehyde compounds, synthetic dyes, etc. have entered the living environment on which human beings depend, and the degree of pollution is increasing day by day. On the one hand, excessive genotoxic substances act on the organism to cause DNA damage. Once the damage exceeds the cell's own repair ability, it will cause cell death; It can also cause varying degrees of damage, which can cause developmental delays...

AI Technical Summary

Problems solved by technology

On the one hand, excessive genotoxic substances act on the organism to cause DNA damage. Once the damage exceeds the cell's own repair ability, it will cause cell death; It can also cause varying degrees of damage, which can cause developmental delays or even stop

For the detection of genetic toxicants in the environment, chemical detection methods are mainly used at present: although this method can quantitatively describe the pollutant content in the environment or in organisms, most of them are limited to the detection of a single harmful substance, and most of them require special large-scale Analytical instruments and analytical methods are costly; it is difficult to detect various pollutants in the environment and evaluate the impact on living cells after their interaction

[0003] Existing studies have shown that environmental pollutants can easily damage biological macromolecules such as DNA in silkworm egg embryo cells, thereby causing developmental delay or even arrest.

The different development stages of the silkworm embryo can be divided into (A: late diapause period, B1: after diapause period, B2: stretching period, C1: before the longest period, C2: the longest period, D 1: hypertrophic stage, D 2: onset of protrusion, E 1: early development of protrusion, E 2: late development of protrusion, E 3: shortening period, E 1 inversion period, E 2: terminal inversion, E 3: trachea Formation stage, E4: eye point stage, E5: turning green stage) and other embryos, but there is no method for detecting genotoxic substances in the environment by utilizing the different environmental sensitivity of silkworm embryos

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