Greenhouse tomato overwintering cultivation method suitable for north alpine regions

A cultivation method and technology in alpine regions, applied in the agricultural field, can solve the problems of soil compaction, salinization, restriction, etc., and achieve the effects of reducing soil-borne diseases, reducing pesticide pollution, and inhibiting reproduction.

Active Publication Date: 2015-12-16
CHIFENG HERUN AGRI HIGH TECH IND DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, facility agriculture in the alpine regions of northern my country has developed rapidly, and the production area of ​​facility agriculture dominated by solar greenhouse cultivation has continued to expand, becoming an important production method for the development of modern agriculture in many areas and driving farmers to increase their income and become rich; tomato has high nutritional value, It is deeply loved by people and is also the main type of vegetable cu

Method used

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  • Greenhouse tomato overwintering cultivation method suitable for north alpine regions
  • Greenhouse tomato overwintering cultivation method suitable for north alpine regions
  • Greenhouse tomato overwintering cultivation method suitable for north alpine regions

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0061] Preparation Example 1: Preparation of Formula I Compound

[0062] (1) Add 2mmol indoloquinone, 10ml acetone, and 2.5mmol potassium carbonate to a 25ml single-necked flask, slowly add 3mmol ethyl bromoacetate dropwise at room temperature, heat up and reflux for reaction, stop the reaction after 4 hours, and react after cooling to room temperature The solution was poured into ice water, and a large amount of white solid was precipitated, which was filtered by suction, washed with water, and dried to obtain a white solid with a yield of 70%.

[0063] (2) Add 2 mmol of the product prepared in step (1) and 15 ml of absolute ethanol to a 25 ml one-mouth bottle, add 9 mmol of 80% hydrazine hydrate under stirring, and heat up to reflux reaction; stop the reaction after 2 hours, cool to room temperature, and suction filter , dried to obtain a white solid, and recrystallized from absolute ethanol to obtain a white solid with a yield of 65%.

[0064] (3) Add 1 mmol of the produc...

Embodiment 1

[0118] Activity experiment (1):

[0119] The PPARδ activity of the compound represented by formula I of the present invention was confirmed by transfection assay. In addition, selectivity for the PPAR subtypes PPARα and PPARγ was also examined. Cytotoxicity was tested by MTT assay, and in vivo activity was studied by animal experiments.

[0120] CV-1 cells were used in this assay. The cells were inoculated into 96-well plates containing DMEM supplemented with 10% FBS, DBS (non-specific bovine serum, defatted) and 1% penicillin / streptomycin, and incubated at 37°C, 5% CO 2 cultured in an incubator. Experiments were performed following the steps of inoculation, transfection, sample application and confirmation. Specifically, CV-1 cells were seeded into a 96-well plate (5000 cells / well), and transfected 24 hours later. Full-length PPAR plasmid DNA, reporter DNA to confirm PPAR activity due to luciferase activity, β-galactosidase DNA to provide information on transfection effi...

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Abstract

The invention discloses a greenhouse tomato overwintering cultivation method suitable for north alpine regions. The method includes the steps of greenhouse preparation, sowing and seedling cultivation, planting, plant adjustment, pollination, fertilizer and water management, disease and pest prevention, harvesting and the like. By means of the cultivation management method, the temperature of a greenhouse can be remarkably raised, diseases and pests can be reduced, safe and efficient greenhouse tomato overwintering production in cold winter in the north alpine regions is achieved, the requirement for tomatoes in local and surrounding markets in winter is met, and development of the regional facility agricultural industry is driven.

Description

technical field [0001] The invention relates to the field of agriculture, and more specifically relates to a method for overwintering cultivation of greenhouse tomatoes suitable for northern alpine regions. Background technique [0002] In recent years, facility agriculture in the alpine regions of northern my country has developed rapidly, and the production area of ​​facility agriculture dominated by solar greenhouse cultivation has continued to expand, becoming an important production method for the development of modern agriculture in many areas and driving farmers to increase their income and become rich; tomato has high nutritional value, It is deeply loved by people and is also the main type of vegetable cultivated in facilities; with the improvement of living standards, people have higher and higher requirements for the quality and safety of vegetable products. Pesticides and hormones lead to excessive pesticide residues in vegetables, soil compaction and salinization,...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G13/00C07D403/06A01N43/653A01P1/00
CPCA01G13/00A01G22/00A01N43/653C07D403/06
Inventor 赵广叡常瑞青夏峰张红霞刘志宇陈勇
Owner CHIFENG HERUN AGRI HIGH TECH IND DEV
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