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Immune vaccine for preventing toxoplasma gondii infection

An immunization vaccine and Toxoplasma gondii technology, which is applied in the field of immunization vaccines, can solve problems such as premature birth, stillbirth, and economic losses in animal husbandry, and achieve the effects of prolonging survival time, reducing the number, and achieving good immune effects.

Active Publication Date: 2015-12-23
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, Toxoplasma gondii infects livestock, which can cause symptoms such as abortion, premature birth, stillbirth, and teratogenicity of sick livestock, which will bring huge economic losses to the development of animal husbandry.
There is still no ideal drug for the treatment of toxoplasmosis, so the development of safe and effective anti-toxoplasma vaccine is particularly important in the prevention and treatment of toxoplasmosis

Method used

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  • Immune vaccine for preventing toxoplasma gondii infection
  • Immune vaccine for preventing toxoplasma gondii infection
  • Immune vaccine for preventing toxoplasma gondii infection

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Construction of prokaryotic expression vector pET30a- erk 7

[0026] 1. Extraction of total RNA of Toxoplasma gondii GT1: Take out the Toxoplasma gondii GT1 strain from liquid nitrogen, thaw it at 37°C and inoculate several BALB / c female mice intraperitoneally. After three consecutive passages, the ascites of the diseased BALB / c mice was collected aseptically, and centrifuged at 9000 rpm for 10 min at room temperature. The precipitation was used for the extraction of GT1 total RNA, and the extraction method was carried out according to the instruction manual of the RNAprepPure animal tissue total RNA extraction kit of Beijing Tiangen Biochemical Technology Co., Ltd.

[0027] 2. Toxoplasma gondii erk Specific amplification of 7 genes: using the total RNA of Toxoplasma gondii GT1 as a template, referring to the instructions of PrimeScript? OneStepRT-PCR kit (TaKaRa), specific amplification erk 7 Target gene, the reaction system is: PrimeScript? OneStepEnzymeMix ...

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Abstract

The invention provides an immunogenicity composition which comprises erk7 recombinant protein and adjuvant. The invention further provides an immune vaccine for preventing toxoplasma gondii infection. The effective component of the immune vaccine is optional one of the immunogenicity compositions in claims 1-4. The immune vaccine has the advantages that the vaccine can effectively prolong the survival time of toxoplasma gondii GT1 acute infection BALB / c mice, the vaccine has no evident effect on PRU acute infection but can greatly reduce brain cyst number in toxoplasma gondii PRU acute infection, and a good immune effect is achieved.

Description

technical field [0001] The invention relates to an immune vaccine for the prevention of toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii ( Toxoplasmagondii ) is a strictly intracellular parasitic protozoan, which is distributed worldwide and can infect all warm-blooded animals including humans and animals. It is a typical pathogen of zoonotic parasitic diseases. About 35% of the world's population is infected with Toxoplasma gondii, and the infection rate is even higher in some areas. Toxoplasma gondii infection is mostly recessive, and it is most harmful to immunocompromised or deficient patients such as organ transplant patients, AIDS and malignant tumor patients, and pregnant women. At the same time, Toxoplasma gondii infects livestock, which can cause symptoms such as abortion, premature birth, stillbirth, and teratogenicity of sick livestock, which will bring huge economic losses to the development of animal husbandry. There is still no id...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/002A61P33/02C12N15/12C12N15/70C12N1/21C12R1/19
Inventor 李中原赵禹朱兴全徐民俊
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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