A kind of preparation method of artificial xenogeneic skin

A technology of heterogeneous skin, applied in the field of preparation of artificial heterogeneous skin, to achieve the effect of avoiding excessive scar formation, complete finished product shape, and improving strength

Active Publication Date: 2018-02-27
上海泽充生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The invention not only solves the problem of industrial waste disposal, but also solves the problem of the source of artificial skin

Method used

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  • A kind of preparation method of artificial xenogeneic skin

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Embodiment 1

[0029] A preparation method of artificial heterogeneous skin, comprising the following steps:

[0030] (1) Take a 12-month-old pig that has passed the quarantine inspection, open the cavity aseptically, take out the thoracic aorta, rinse it with medical normal saline, and bluntly dissect to obtain the intima with a thickness of 0.3-0.5mm, and then wash it with medical normal saline , to obtain the intima of porcine thoracic aorta;

[0031] (2) Place the porcine thoracic aortic intima obtained in step (1) in 1M aqueous sodium chloride solution, and vibrate at a speed of 100 r / min in a constant temperature shaker at 37 ° C, and ultrasonically vibrate every 6 hours (frequency 40KHz) for 5 minutes, rinse with medical saline after 24 hours;

[0032] (3) Place the porcine thoracic aortic intima obtained in step (2) in an aqueous solution of sodium lauryl sulfate with a solubility of 10 mg / mL, and vibrate at a speed of 100 r / min in a constant temperature shaker at 37°C , ultrasonic...

Embodiment 2

[0039] A preparation method of artificial heterogeneous skin, comprising the following steps:

[0040] (1) Take an 18-month-old pig that has passed the quarantine, aseptically open the cavity, take the thoracic aorta, rinse it with medical normal saline, and bluntly dissect to obtain the intima with a thickness of 0.3-0.5 mm, and then wash it with medical normal saline , to obtain the intima of porcine thoracic aorta;

[0041] (2) Place the porcine thoracic aortic intima obtained in step (1) in 1M aqueous sodium chloride solution, and vibrate at a speed of 150 r / min in a constant temperature shaker at 37 ° C, and ultrasonically vibrate every 6 hours (frequency 40KHz) for 5 minutes, rinse with medical saline after 24 hours;

[0042] (3) Place the porcine thoracic aortic intima obtained in step (2) in an aqueous solution of sodium dodecyl sulfate with a solubility of 10 mg / mL, and vibrate at a speed of 150 r / min in a constant temperature shaker at 37°C , ultrasonic vibration (...

Embodiment 3

[0049] A preparation method of artificial heterogeneous skin, comprising the following steps:

[0050] (1) Take a 24-month-old pig that has passed the quarantine, aseptically open the cavity, take the thoracic aorta, rinse it with medical normal saline, and bluntly separate the intima with a thickness of 0.3-0.5mm, and then wash it with medical normal saline , to obtain the intima of porcine thoracic aorta;

[0051] (2) Place the porcine thoracic aortic intima obtained in step (1) in 1M aqueous sodium chloride solution, and vibrate at a speed of 120 r / min in a constant temperature shaker at 37 ° C, and ultrasonically vibrate every 6 hours (frequency 40KHz) for 5 minutes, rinse with medical saline after 24 hours;

[0052] (3) Place the porcine thoracic aortic intima obtained in step (2) in an aqueous solution of sodium lauryl sulfate with a solubility of 10 mg / mL, and vibrate at a speed of 120 r / min in a constant temperature shaker at 37°C , ultrasonic vibration (frequency 40...

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Abstract

The invention discloses a preparation method of artificial xenogeneic skin. In the method, the intima of the blood vessel is obtained after processing the pig thoracic aorta, and then passed through 1M sodium chloride solution, 10mg / mL sodium lauryl sulfate solution and 2.5mg / mL of trypsin solution, rinsed, and finally cut into a suitable size according to clinical needs, and sterilized by electron beams, the artificial xenogeneic skin was obtained. The artificial skin of the invention has rich sources of raw materials, short production period, long storage time and low price, and has great potential for clinical application.

Description

technical field [0001] The invention relates to the technical field of tissue engineering biomaterials, in particular to a method for preparing artificial heterogeneous skin from pig thoracic aorta. Background technique [0002] The skin is an organ with a high-level tissue structure covering the surface of the human body. It has the functions of protecting the human body, preventing bacterial erosion and infection, regulating body temperature, and excreting waste fluids. When the skin is traumatized, burned or otherwise damaged, it will cause tissue fluid loss. This will lead to the imbalance of water, electrolyte and acid-base balance in the human body, affecting the normal absorption and metabolic balance of the human body. In addition, wounds exposed to the air are highly susceptible to bacterial infection, which can lead to shock or sepsis in severe cases. Therefore, the repair of the skin must be solved. [0003] An ideal artificial skin should have the following cha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/36A61L27/38A61L27/54
Inventor 邓超付海田尚京迎陈敬华
Owner 上海泽充生物技术有限公司
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