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Method of extracting and purifying anthocyanin from suaeda salsa

A technology of Suaeda salsa and anthocyanins, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problem of low energy consumption and achieve the effect of simplifying the process

Inactive Publication Date: 2015-12-23
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: Aiming at the limitations of the current use of natural pigments and the deficiencies of the prior art, the present invention provides a pigment extraction raw material with a wide range of sources, large biomass, economy, and simple natural components, with simple extraction steps and low energy consumption. The extraction method and the separation and purification process solve the bottleneck problem of using natural pigments, and provide a promising way for the promotion and use of natural food pigments and the resource development and utilization of the halophyte Suaeda salsa

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  • Method of extracting and purifying anthocyanin from suaeda salsa
  • Method of extracting and purifying anthocyanin from suaeda salsa
  • Method of extracting and purifying anthocyanin from suaeda salsa

Examples

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Embodiment 1

[0026] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10g of the sample and add 400ml of deionized water, leaching at 40°C for 50min, suction filtration to obtain the filtrate, and centrifuge at 10000×g for 10min , after taking its supernatant, leaching once again according to the above steps, and combining the two leaching supernatants; the absorbance of the obtained extract is 0.228; the supernatant is absorbed and desorbed by AB-8 type macroporous adsorption resin, and the crude Load the extract and stock solution, the adsorption time is 1.5h, the adsorption flow rate is 1mL / min, the sample volume is 60mL, the elution rate is 2.5mL / min, and the ethanol solution with a volume fraction of 50% is desorbed for 1h to obtain the purified Anthocyanin extract: centrifuge the anthocyanin extract extracted and purified by the above process at 12,000×g for 10 min, then take the supernatant, concentrate under redu...

Embodiment 2

[0028] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10g of the sample and add 400ml of 5% ethanol solution, extract at 40°C for 50min, and filter to obtain the filtrate, 10000×g Centrifuge for 10 minutes, take the supernatant and extract it again according to the above steps, and combine the two extraction supernatants; the absorbance of the obtained extract is 0.221; the supernatant is adsorbed and desorbed by AB-8 macroporous adsorption resin, Load the sample with the crude extract, the adsorption time is 2h, the adsorption flow rate is 1mL / min, the sample volume is 60mL, the elution speed is 2.5mL / min, and the ethanol solution with a volume fraction of 50% is used for desorption for 1.5h, and the purified The anthocyanin extract after extraction and purification by the above-mentioned process is centrifuged at 12000×g for 10 min, and the supernatant is concentrated under reduced pressure and freeze-d...

Embodiment 3

[0030] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve. Take 10g sample and add 400ml of 10% ethanol solution, extract at 40°C for 50min, filter with suction to get the filtrate, centrifuge at 10000×g for 10min, take the supernatant and extract once more according to the above steps, combine the two extracts supernatant; the absorbance of the obtained extract was 0.214; the supernatant was adsorbed and desorbed by AB-8 macroporous adsorption resin, and the sample was loaded with the crude extract stock solution, the adsorption time was 1.5h, the adsorption flow rate was 1mL / min, and the sample volume was 60mL, the elution rate is 2.5mL / min, desorb with ethanol solution with a volume fraction of 50%, and the time is 1.5h to obtain the purified anthocyanin extract; the anthocyanin extract extracted and purified by the above process After being centrifuged at 12000×g for 10 min, the supernatant was concentrated un...

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Abstract

The invention discloses a method of extracting and purifying anthocyanin from suaeda salsa. The method comprises the steps of preprocessing of raw materials, digestion, purification and drying. The method is used for extracting the natural pigment of anthocyanin with biological healthcare functions from the suaeda salsa which is halophyte widely distributed on saline land at coastal mudflat. The method overcomes the defects that current sources of raw materials for anthocyanin extraction are limited, extraction processes are tedious, and much energy is consumed; moreover, since suaeda salsa plants are low in sugar content, tedious desugarization processes are not needed, and the technological process is simplified. Meanwhile, by means of the process, tests confirm that AB-8 macroporous adsorption resin is the optimal resin for purifying pigments; after the anthocyanin is subjected to purification, the color value of the anthocyanin is increased to 5.6 which greatly exceeds the national standard requirement of 3.0 with respect to natural pigments in food additives.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a method for extracting and purifying anthocyanins from Suaeda salsa. Background technique [0002] Suaeda salsa is a typical true halophyte, which is widely distributed on saline land such as coastal tidal flats. It is a natural resource with great development potential and has great economic, ecological and social benefits. Studies have confirmed that the rich red pigment contained in Suaeda salsa is an anthocyanin pigment. [0003] With the development of society and economy, people pay more and more attention to health at present, and it has become the trend of the times to replace synthetic pigments with natural pigments. Anthocyanins have been used for a long time as a natural food coloring in our country. Studies have also confirmed that anthocyanins can also promote the regeneration of rhinoplasty and improve microcirculation, thus achieving good curative effect in ...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 彭斌许伟邵荣赵云锋封功能
Owner YANCHENG INST OF TECH
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