Method of extracting and purifying anthocyanin from suaeda salsa

A technology of Suaeda salsa and anthocyanins, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problem of low energy consumption and achieve the effect of simplifying the process

Inactive Publication Date: 2015-12-23

YANCHENG INST OF TECH

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Problems solved by technology

[0005] Purpose of the invention: Aiming at the limitations of the current use of natural pigments and the deficiencies of the prior art, the present invention provides a pigment extraction raw material with a wide range of sources, large biomass, economy, and simple natural components, with simple extraction ...

Abstract

The invention discloses a method of extracting and purifying anthocyanin from suaeda salsa. The method comprises the steps of preprocessing of raw materials, digestion, purification and drying. The method is used for extracting the natural pigment of anthocyanin with biological healthcare functions from the suaeda salsa which is halophyte widely distributed on saline land at coastal mudflat. The method overcomes the defects that current sources of raw materials for anthocyanin extraction are limited, extraction processes are tedious, and much energy is consumed; moreover, since suaeda salsa plants are low in sugar content, tedious desugarization processes are not needed, and the technological process is simplified. Meanwhile, by means of the process, tests confirm that AB-8 macroporous adsorption resin is the optimal resin for purifying pigments; after the anthocyanin is subjected to purification, the color value of the anthocyanin is increased to 5.6 which greatly exceeds the national standard requirement of 3.0 with respect to natural pigments in food additives.

Application Domain

Organic chemistryNatural dyes

Technology Topic

ChemistryDigestion +9

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Examples

Experimental program(10)

Example Embodiment

[0025] Example 1

[0026] Take the fresh Suaeda salsa to remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10 g of the sample, add 400 ml of deionized water, extract at 40 ° C for 50 minutes, and filter with suction to obtain the filtrate, which is centrifuged at 10,000 × g for 10 minutes , take the supernatant and then extract it again according to the above steps, and combine the two extraction supernatants; the absorbance of the obtained extract is 0.228; The original solution of the extract was loaded, the adsorption time was 1.5h, the adsorption flow rate was 1mL/min, the sample volume was 60mL, the elution rate was 2.5mL/min, and the purified ethanol solution was desorbed with a volume fraction of 50% for 1h. The anthocyanin extract; the anthocyanin extract extracted and purified by the above process is centrifuged at 12,000 × g for 10 min, the supernatant is concentrated under reduced pressure, and freeze-dried to obtain purified anthocyanin powder.

Example Embodiment

[0027] Example 2

[0028] Take the fresh Suaeda salsa to remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10g of the sample, add 400ml of 5% ethanol solution, extract at 40°C for 50min, and filter with suction to obtain the filtrate, 10000×g Centrifuge for 10 min, take the supernatant and extract it again according to the above steps, and combine the two extraction supernatants; the absorbance of the obtained extract is 0.221; the supernatant is adsorbed and desorbed by AB-8 macroporous adsorption resin, The crude extract was loaded with the original solution, the adsorption time was 2h, the adsorption flow rate was 1mL/min, the sample volume was 60mL, and the elution rate was 2.5mL/min. After the anthocyanin extract extracted and purified by the above process is centrifuged at 12,000 × g for 10 min, the supernatant is concentrated under reduced pressure, and freeze-dried to obtain the purified anthocyanin. powder.

Example Embodiment

[0029] Example 3

[0030] Take the fresh Suaeda salsa to remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve. Take 10g of sample and add 400ml of 10% ethanol solution, leaching at 40℃ for 50min, suction filtration to obtain the filtrate, centrifuge at 10000×g for 10min, take the supernatant and leaching again according to the above steps, and combine the two leaching The absorbance of the obtained extract was 0.214; the supernatant was adsorbed and desorbed by AB-8 macroporous adsorption resin, and the crude extract was loaded with the original solution, the adsorption time was 1.5h, the adsorption flow rate was 1mL/min, and the sample volume was 60mL, elution rate 2.5mL/min, desorb with 50% ethanol solution, time 1.5h, to obtain purified anthocyanin extract; extract and purify the anthocyanin extract by the above process After centrifugation at 12,000 × g for 10 min, the supernatant was concentrated under reduced pressure and freeze-vacuum-dried to obtain purified anthocyanin powder.

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Method of extracting and purifying anthocyanin from suaeda salsa

A technology of Suaeda salsa and anthocyanins, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problem of low energy consumption and achieve the effect of simplifying the process

AI-Extracted Technical Summary

Problems solved by technology

Abstract

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Examples

Example Embodiment

Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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