Method of extracting and purifying anthocyanin from suaeda salsa
A technology of Suaeda salsa and anthocyanins, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problem of low energy consumption and achieve the effect of simplifying the process
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Embodiment 1
[0026] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10g of the sample and add 400ml of deionized water, leaching at 40°C for 50min, suction filtration to obtain the filtrate, and centrifuge at 10000×g for 10min , after taking its supernatant, leaching once again according to the above steps, and combining the two leaching supernatants; the absorbance of the obtained extract is 0.228; the supernatant is absorbed and desorbed by AB-8 type macroporous adsorption resin, and the crude Load the extract and stock solution, the adsorption time is 1.5h, the adsorption flow rate is 1mL / min, the sample volume is 60mL, the elution rate is 2.5mL / min, and the ethanol solution with a volume fraction of 50% is desorbed for 1h to obtain the purified Anthocyanin extract: centrifuge the anthocyanin extract extracted and purified by the above process at 12,000×g for 10 min, then take the supernatant, concentrate under redu...
Embodiment 2
[0028] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve; take 10g of the sample and add 400ml of 5% ethanol solution, extract at 40°C for 50min, and filter to obtain the filtrate, 10000×g Centrifuge for 10 minutes, take the supernatant and extract it again according to the above steps, and combine the two extraction supernatants; the absorbance of the obtained extract is 0.221; the supernatant is adsorbed and desorbed by AB-8 macroporous adsorption resin, Load the sample with the crude extract, the adsorption time is 2h, the adsorption flow rate is 1mL / min, the sample volume is 60mL, the elution speed is 2.5mL / min, and the ethanol solution with a volume fraction of 50% is used for desorption for 1.5h, and the purified The anthocyanin extract after extraction and purification by the above-mentioned process is centrifuged at 12000×g for 10 min, and the supernatant is concentrated under reduced pressure and freeze-d...
Embodiment 3
[0030] Take the picked fresh Suaeda salsa, remove the roots, wash, dry, cut into pieces, and pass through a 40-mesh sieve. Take 10g sample and add 400ml of 10% ethanol solution, extract at 40°C for 50min, filter with suction to get the filtrate, centrifuge at 10000×g for 10min, take the supernatant and extract once more according to the above steps, combine the two extracts supernatant; the absorbance of the obtained extract was 0.214; the supernatant was adsorbed and desorbed by AB-8 macroporous adsorption resin, and the sample was loaded with the crude extract stock solution, the adsorption time was 1.5h, the adsorption flow rate was 1mL / min, and the sample volume was 60mL, the elution rate is 2.5mL / min, desorb with ethanol solution with a volume fraction of 50%, and the time is 1.5h to obtain the purified anthocyanin extract; the anthocyanin extract extracted and purified by the above process After being centrifuged at 12000×g for 10 min, the supernatant was concentrated un...
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