Bacillus coagulans preparation for livestock breeding and preparation method thereof
A technology of Bacillus coagulans, livestock and poultry breeding, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems affecting human health, ecological environment and safety, destroying the microbial flora of the digestive tract of livestock and poultry, etc. Achieve the effects of improving the intestinal micro-ecological environment, improving breeding efficiency, and enriching raw materials
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Embodiment 1
[0042] Embodiment 1: (recipe and preparation method 1 of product of the present invention)
[0043] A microbial preparation for livestock and poultry breeding-Bacillus coagulans, prepared according to the following method steps:
[0044] (1) Preparation of medium at all levels, wherein:
[0045] a) PDA slant solid medium: 200g of potatoes, 2g of glucose, 2g of agar, 1 liter of water, set aside;
[0046] b) shake flask liquid culture medium: 200g of potatoes, 2g of glucose, 1 liter of water, set aside;
[0047] c) Seed tank fermentation medium: 20 grams of soluble starch, 7 grams of yeast extract, 10 grams of peptone, 10 grams of soybean meal, 953 grams of water, set aside;
[0048] d) Fermentation tank fermentation medium: 20 grams of soluble starch, 7 grams of yeast extract, 10 grams of peptone, 10 grams of soybean meal, 953 grams of water, set aside;
[0049] (2) Cultivation of slant strains: Inoculate the preservation number: CCTCCNo: M2011422 Bacillus coagulans BFS-2 st...
Embodiment 2
[0053] The preparation of the seed tank and fermenter fermentation medium in this embodiment: 30 grams of soluble starch, 5 grams of yeast extract, 5 grams of peptone, 15 grams of soybean meal, and 945 grams of water for later use; the rest of the preparation methods are the same as in Example 1. The number of live bacteria is 2X10 8 CFU / g.
Embodiment 3
[0054] Embodiment 3: (recipe and preparation method 3 of product of the present invention)
[0055] The preparation of the seed tank and fermenter fermentation medium in this embodiment: 28 grams of soluble starch, 5 grams of yeast extract, 5 grams of peptone, 12 grams of soybean meal, and 950 grams of water for later use. All the other preparation methods are the same as in Example 1. The number of live bacteria is 5X10 8 CFU / g.
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