Bacillus coagulans preparation for livestock breeding and preparation method thereof

A technology of Bacillus coagulans, livestock and poultry breeding, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems affecting human health, ecological environment and safety, destroying the microbial flora of the digestive tract of livestock and poultry, etc. Achieve the effects of improving the intestinal micro-ecological environment, improving breeding efficiency, and enriching raw materials

Inactive Publication Date: 2015-12-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the deepening of understanding, it has been found that long-term use of antibiotics will destroy the microbial flora in the digestive tract of livestock and poultry, make the animal body resistant to antibiotics, and the residues of antibiotics in livestock and poultry products have directly affected human health. Health and Ecological Safety

Method used

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  • Bacillus coagulans preparation for livestock breeding and preparation method thereof
  • Bacillus coagulans preparation for livestock breeding and preparation method thereof
  • Bacillus coagulans preparation for livestock breeding and preparation method thereof

Examples

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Effect test

Embodiment 1

[0042] Embodiment 1: (recipe and preparation method 1 of product of the present invention)

[0043] A microbial preparation for livestock and poultry breeding-Bacillus coagulans, prepared according to the following method steps:

[0044] (1) Preparation of medium at all levels, wherein:

[0045] a) PDA slant solid medium: 200g of potatoes, 2g of glucose, 2g of agar, 1 liter of water, set aside;

[0046] b) shake flask liquid culture medium: 200g of potatoes, 2g of glucose, 1 liter of water, set aside;

[0047] c) Seed tank fermentation medium: 20 grams of soluble starch, 7 grams of yeast extract, 10 grams of peptone, 10 grams of soybean meal, 953 grams of water, set aside;

[0048] d) Fermentation tank fermentation medium: 20 grams of soluble starch, 7 grams of yeast extract, 10 grams of peptone, 10 grams of soybean meal, 953 grams of water, set aside;

[0049] (2) Cultivation of slant strains: Inoculate the preservation number: CCTCCNo: M2011422 Bacillus coagulans BFS-2 st...

Embodiment 2

[0053] The preparation of the seed tank and fermenter fermentation medium in this embodiment: 30 grams of soluble starch, 5 grams of yeast extract, 5 grams of peptone, 15 grams of soybean meal, and 945 grams of water for later use; the rest of the preparation methods are the same as in Example 1. The number of live bacteria is 2X10 8 CFU / g.

Embodiment 3

[0054] Embodiment 3: (recipe and preparation method 3 of product of the present invention)

[0055] The preparation of the seed tank and fermenter fermentation medium in this embodiment: 28 grams of soluble starch, 5 grams of yeast extract, 5 grams of peptone, 12 grams of soybean meal, and 950 grams of water for later use. All the other preparation methods are the same as in Example 1. The number of live bacteria is 5X10 8 CFU / g.

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Abstract

The invention discloses a bacillus coagulans preparation for livestock breeding and a preparation method thereof and belongs to the technical field of additives for livestock breeding. The preparation is prepared through the following steps: 2-5% of soluble starch, 0.5-1% of yeast extract, 0.5-1.5% of peptone, 1.0-1.5% of soybean meal and the balance of water are adopted to prepare a fermentation medium; with the BFS-2 strains of the bacillus coagulans with the preservation number of CCTCC No.M 2011422 as strains, carrying out all-stage fermentation, so as to obtain the bacillus coagulans preparation. The preparation is low in price, and high in content of bacterial count, the guarantee period is one year or above, and after the preparation is used, the growth of harmful bacteria in the intestines of animals can be effectively restrained, the micro-ecological environment of the intestines tracts of bred animals is improved, and the incidence of diarrhea is reduced; meanwhile, the utilization of the feed is effectively promoted, the breeding cost is reduced, the culture benefits are increased and the preparation can be popularized and applied in livestock farms.

Description

technical field [0001] The invention relates to the field of livestock and poultry breeding additives, in particular to a microbial preparation for livestock and poultry breeding-bacillus coagulans and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of intensive and large-scale aquaculture, the outbreaks of aquaculture animal diseases have become more and more frequent, and the damage has become more and more serious. Aquaculture animal diseases have become a bottleneck restricting the development of aquaculture. [0003] Antibiotics and other chemical agents have been used in the aquaculture industry for decades, and they have played a positive role in preventing animal diseases and improving the output and quality of the aquaculture industry. However, with the deepening of understanding, it has been found that long-term use of antibiotics will destroy the microbial flora in the digestive tract of livestock and poultry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/07
Inventor 吴逸飞孙宏姚晓红王新柳永汤江武杨家帅
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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