Herpesviridae strain

Abstract

The invention discloses a herpesviridae strain which is characterized in that a preservation number of the herpesviridae strain is CCTCC (China Center For Type Culture Collection) NO:V201314; a PRV/HN2012 strain is inherited to the seventh generation, the seventh-generation virus is subjected to determination of a virus titer TCID50 (Tissue Culture Infectious Dose), and final result shows that the TCID50 is 10<9.0>; the PRV/HN2012 strain is inoculated to mice, and after the inoculated virus is challenged for 24h, the mice in a PRV/HN2012 strain infected group are found with neurologic symptoms; after the inoculated virus is challenged for 48h, all the mice are found to die, experimental results show that the strain has high toxicity; based on the results, the research of physicochemical properties of the PRV/HN2012 strain indicates that the strain is sensitive to analytically-pure chloroform and belongs to a virus with an envelope; with higher resistance to acid and alkali, the strain can be deactivated after being treated with hydrochloric acid with the pH value of 3.0 and NaOH with the pH value of 11.0; with higher resistance to heat, the strain can be deactivated after being treated in a water bath with 56DEG C for 1h; the strain is sensitive to trypsin, so that the influence of ultraviolet rays on the infectivity of the strain is not obvious after the strain is treated by the ultraviolet rays for 30min.

Description

technical field [0001] The invention relates to a porcine pseudorabies virus strain. Background technique [0002] Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV) in domestic animals and a variety of wild animals, with fever, itching (except pigs) and encephalomyelitis as the main symptoms . The disease was first seen in the United States in 1813. Because its clinical manifestations are similar to rabies, it is called pseudorabies. In 1902, the Hungarian scholar Aujeszky first reported the disease, so the disease was also named Aujeszky's disease. Pigs are the natural host and main source of infection of the disease. PRV can infect pigs of different ages, but the most serious infection is in pregnant sows and suckling piglets: leading to abortion, stillbirth and mummified fetuses in pregnant sows; obvious neurological symptoms in newborn piglets Symptoms, paralysis, exhaustion and death are mostly acute fatal processes, and the mortalit...

AI Technical Summary

Problems solved by technology

Since the spring of 2012, there have been suspected cases of PR in pig farms immunized with PRV gene-deficient vac...

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