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A method for rapid identification of Dendrobium Huoshanense

A technology of Dendrobium Huoshanense and Dendrobium, which is applied in the field of identification of germplasm sources of traditional Chinese medicine, can solve the problems that important identification characteristics have not been reported, there are few studies on Dendrobium Huoshanense, and the identification of Dendrobium Huoshanense has not been recorded.

Active Publication Date: 2018-07-06
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, many scholars have studied the authenticity of Dendrobium medicinal materials, but most of them focus on the identification of Dendrobium candidum, while there are relatively few studies on Dendrobium Huoshanense
The 2010 edition of Pharmacopoeia only provides the traditional microscopic identification method or thin layer identification method for several mainstream Dendrobium in the market (D. no record
However, the important identification features of the microstructure of Dendrobium Huoshanense and Dendrobium Henan have not been reported yet.

Method used

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  • A method for rapid identification of Dendrobium Huoshanense
  • A method for rapid identification of Dendrobium Huoshanense
  • A method for rapid identification of Dendrobium Huoshanense

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: The identification of the character and microscopic characteristics of Dendrobium Huoshanense has been determined

[0037] 1. Materials and Reagents

[0038] In October 2014, fresh stems of Dendrobium huoshanense were purchased from Huoshan. After being identified by Professor Peng Huasheng of Anhui University of Traditional Chinese Medicine, they are all fresh stems of Dendrobium huoshanense, a plant in the family Orchidaceae.

[0039] FAA solution, alcohol (70%, 80%, 85%, 90%, 95%, 100%), xylene, paraffin, neutral gum, safranin-fast green dye solution;

[0040] 2. Instruments and software

[0041] Vernier caliper with a range of 1-150mm (Shanghai Measuring Tool and Cutting Tool Factory, Shanghai, China);

[0042] Leica DM6000 B microscope (Leica, Benshein, Germany);

[0043] Olympus BH-2 microscope;

[0044] Leica RM2265 rotary microtome;

[0045] LAS software (Leica Applications Suite V4.1);

[0046] Photoshop CS6; Excel; SPSS17.0; AI CS6

[0047] 3...

Embodiment 2

[0071] Embodiment 2: the character and the microscopic feature identification of unknown Dendrobium huoshanense

[0072] 1. Materials and Reagents

[0073] Ten batches of Dendrobium samples were purchased from Huoshan, Anhui.

[0074] Reagent is with embodiment 1.

[0075] 2. Instruments and software

[0076] Same as Example 1

[0077] 3. Experimental steps

[0078] (1) Select 20 sample stems from each batch of Dendrobium to be tested and place them on the test bench, cut off each section with a blade and number them, starting from the base of the stem, numbering starts from 1, and then increases sequentially; use 1- The length and diameter of each segment were measured and recorded with a vernier caliper with a range of 150 mm; the measured diameter was the middle of each segment. Then input the obtained data into an Excel table to make a line graph; generate SPSS data again from the data, and use SPSS17.0 to make a box graph.

[0079] Compare the diameter and length tr...

Embodiment 3

[0090] Embodiment 3: the identification of market easy-to-confuse product Henan Dendrobium

[0091] 1. Materials and reagents

[0092] In October 2014, fresh stems of Dendrobium henanense were purchased from Huoshan. After being identified by Professor Peng Huasheng of Anhui University of Traditional Chinese Medicine, they were all fresh stems of the orchid plant Dendrobium henanense.

[0093] FAA solution, alcohol (70%, 80%, 85%, 90%, 95%, 100%), xylene, paraffin, neutral gum, safranin-fast green dye solution;

[0094] 2. Instruments and software

[0095] With embodiment 1.

[0096] 3. Experimental steps

[0097] With embodiment 1.

[0098] 4. Results and Analysis

[0099] Characters and characteristics of Henan Dendrobium: from figure 2 It can be seen that the length of each node of Dendrobium Henan is between 3.2-20.1 mm, and the overall change trend from the base to the shoot tip is to increase first and then decrease, and the length of the second or third node reac...

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Abstract

The invention relates to a method for quickly identifying Dendrobium Huoshanense. The operation steps are as follows: (1) Measure the diameter and length of each node of Dendrobium tested, and compare it with the characteristics of Dendrobium Huoshanense; (2) Slice each node of Dendrobium tested by hand, observe with a microscope, take pictures, and digitally The number of vascular bundles is compared with the characteristics of Dendrobium Huoshanense; (3) Paraffin sections are made on the tested Dendrobium; (4) The paraffin sections are observed under a microscope to see the type of vascular bundle sheath, whether there are siliceous blocks, whether there are Needle crystal bundles and the location of needle crystal bundles, etc., and compared with the characteristics of Dendrobium Huoshanense; determine whether the tested Dendrobium belongs to Dendrobium Huoshanense. The present invention adopts the method of combining traits and microscopic characteristics to quickly identify Dendrobium Huoshanense, which is simple, fast, efficient, practical and economical, can realize timely identification of Dendrobium Huoshanense, and provides guarantee for accurate clinical drug use and quality assurance.

Description

technical field [0001] The invention belongs to the technical field of identification of germplasm sources of traditional Chinese medicines, and in particular relates to a method for identifying Dendrobium Huoshanense according to its properties and microscopic features. Background technique [0002] Dendrobium is a large genus of Orchidaceae, with more than 1100 species in the world. There are 74 species and 2 varieties of Dendrobium in China, among which more than 40 species of Dendrobium have been used as traditional or folk medicine. Dendrobium is sweet and slightly cold. Beneficial to the stomach, nourishing yin and reducing fever. It is clinically used in febrile diseases, dry mouth and polydipsia, insufficient stomach yin, lack of food and retching, persistent asthenic fever after illness, hyperactivity of fire due to yin deficiency, fatigue and heat due to bone steaming, blurred vision, soft muscles and bones, etc. Among them, the quality of Huoshan Dendrobium is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N21/01
Inventor 彭华胜赵玉姣彭代银陈乃富韩邦兴程铭恩
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE