Heavy metal quantitative detecting system and method for quantitatively detecting heavy metal

A heavy metal and colloidal gold technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, accuracy checking, inaccurate test results, etc., and achieves good application prospects, stable gold-labeled antibodies, and wide application range. Effect

Active Publication Date: 2015-12-30
成都安普诺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, since colloidal gold can only be effectively combined with antibodies in a weakly alkaline environment, once the pH of the detection object is not weakly alkaline, it cannot be accurately detected, and the detection of heavy metals needs to be extracted with strong acid, although by increasing the dilution factor It can be barely detected, but the detection results are not accurate
[0015] Chinese patent application CN201010229009.1 discloses a method for the preparation of heavy metal mercury antibody and enzyme-linked immunosorbent assay for the determination of heavy metal content, which can be used to detect heavy gold and mercury, but the accuracy of the method is poor, and the operation is complicated, requiring professional large-scale equipment
Chinese patent application CN201020521531.2 discloses a detection card for heavy gold, which can only be detected in a special environment, and can only be detected qualitatively, and cannot be detected quantitatively.

Method used

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  • Heavy metal quantitative detecting system and method for quantitatively detecting heavy metal
  • Heavy metal quantitative detecting system and method for quantitatively detecting heavy metal
  • Heavy metal quantitative detecting system and method for quantitatively detecting heavy metal

Examples

Experimental program
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Effect test

Embodiment 1

[0052] The preparation of embodiment 1 modified colloidal gold of the present invention

[0053] Prepare a colloidal gold solution with a diameter of 10-60 nm by chloroauric acid-trisodium citrate reduction method, add 0.1-3 mL of 1% bis(triethanolamine) diisopropyl titanate (TMC) to 100 mL of the prepared colloidal gold -TE) solution, mixed and stirred at 100-400r / min for 0.5-2h. Use ammonia water to adjust the pH value of the solution to 8-10, add 0.1-2mL n-butyl titanate, continue the reaction at room temperature for 24-36 hours, centrifuge at 4000-16000r / min for 5-60min, suck out the supernatant and add Washing with pure water or buffer solution is repeated 1-2 times, and the titanate-modified colloidal gold particles (colloidal gold of the present invention) containing active -OH on the surface are prepared.

Embodiment 2

[0054] Embodiment 2 Preparation of gold-labeled antibody of the present invention

[0055] Take the modified colloidal gold particles prepared in Example 1, add 0.2 to 2 mg of Cd monoclonal antibody per 100 mL, react for 2 hours, centrifuge at 6000 to 12000 r / min for 5 to 60 minutes, remove the supernatant, and use reconstitution solution to determine the solution to 1 / 20 of the volume of the colloidal gold solution to obtain the gold-labeled antibody.

[0056] The antibody is a heavy metal antibody, such as Cd monoclonal antibody, Pb monoclonal antibody, Hg monoclonal antibody, As monoclonal antibody, Cr monoclonal antibody, or Cu monoclonal antibody.

Embodiment 3

[0057] Embodiment 3 The present invention detects the detection system of heavy metal and using method thereof

[0058] 1. Detection system

[0059] 1.1 Preparation of the kit of the present invention

[0060] The structure of the kit of the present invention is as follows: Figure 1~2 As shown: it includes a test paper and a jam, and the test paper is placed in the jam; the test paper includes a plastic bottom plate 1, one end of which is pasted with a sample pad 2, and one end of the upper sample pad is tightly pressed and then contains the antibody labeled with the corresponding heavy metal to be detected. The colloidal gold pad 3 made by Gold Label, one end of the colloidal gold pad 3 is tightly pressed to the nitrocellulose membrane 4, the nitrocellulose membrane is coated with the detection line T and the quality control line C5, and the detection line T is coated with the heavy metal to be tested Complete antigen, quality control line C is coated with anti-mouse IgG a...

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Abstract

The invention discloses a modified colloidal gold preparation method and a gold-labeled antibody preparation method, and further discloses a colloidal gold immunochromatography kit, a heavy metal quantitative detecting system with the kit, and a method for quantitatively detecting heavy metal. A gold-labeled antibody formed by modified colloidal gold and an antibody is stable and is not influenced by the acid environment; when the immunochromatography kit is prepared from the modified colloidal gold, in the acid conditions, heavy metal can still be accurately detected in an oriented mode, traditional atomic absorption spectrometry can be replaced, cost is low, and application prospects are good.

Description

technical field [0001] The invention relates to a heavy metal quantitative detection system and a method for quantitative detection of heavy metals. Background technique [0002] With the rapid development of our country's economy and the improvement of living standards, people have higher requirements for food safety. serious. There are many reasons for food safety problems, and the problem of excessive heavy metals is one of the important factors affecting food safety. At present, heavy metals such as copper, lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver, etc. The heavy metals that are harmful to our humans and the environment at this stage include the following elements: mercury, cadmium, lead, chromium, copper, and metalloid arsenic and other heavy metal elements with significant biological toxicity. [0003] If heavy metal elements are directly discharged into rivers, lakes or seas without treatment, or enter the so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 刘松杰米军吴晓迪刘文福
Owner 成都安普诺生物科技有限公司
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