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A kind of abacavir individualized drug detection kit and method thereof

A technology for detection kits and medicines, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as unsuitable large-scale popularization and use, and unsatisfactory detection methods for HLA-B*5701. , to achieve the effect of easy promotion, short detection time and high accuracy

Active Publication Date: 2019-02-05
BEIJING JINQI BIOLOGICAL TECH CO LTD
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  • Claims
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Problems solved by technology

[0008] The problem to be solved by the present invention is that the existing method for detecting HLA-B*5701 is not ideal and is not suitable for large-scale promotion and use

Method used

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  • A kind of abacavir individualized drug detection kit and method thereof
  • A kind of abacavir individualized drug detection kit and method thereof
  • A kind of abacavir individualized drug detection kit and method thereof

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Embodiment Construction

[0038] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0039] A detection primer for individualized drug use of abacavir, used to detect the G allele of rs2395029, including the following primers:

[0040] 5′-GTGTGACAGCAGCCATGC-3′ (SEQ ID No.1, hereinafter referred to as F3)

[0041] 5'-TCTCCCAAAAACCACACTCTG-3' (SEQ ID No.2, hereinafter referred to as B3)

[0042] 5′-ACCAGCGGGTGAGAAGAGGACCTCCTGGGGATCAGGATC-3′

[0043] (SEQ ID No.3, hereinafter referred to as FIP)

[0044] 5′-GGACAGCTGTAATGTGTAGTTCAATGGAGGGTAGAAGGTCCT

[0045] GGATT-3' (SEQ ID No.4, hereinafter referred to as BIP).

[0046] The above-mentioned F3, B3, FIP, and BIP are primers purified by HPLC purification.

[0047] An abacavir individualized drug detection kit includes a composition for detecting the genotype of the rs2395029 site and further detecting HLA-B*5701. The compositio...

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Abstract

The invention provides an abacavir individualized medication detection kit and method and belongs to the technical field of abacavir medication detection. The Allele-Specific LAMP (loop-mediated isothermal amplification) technology is adopted, specific primers are designed aiming at a G allele of rs2395029, and whether a patient carries HLA (human leukocyte antigen)-B*5701 allele is judged according to the relevance of the G allele of rs2395029 and HLA-B*5701. After detection, whether a positive reaction is performed can be judged through observation of the state change, namely, whether precipitation or color change exists or not, of a reaction system, judgment can be performed with naked eyes, additional device equipment is not needed, and easiness and feasibility are realized.

Description

technical field [0001] The invention relates to the technical field of abacavir drug detection, in particular to an abacavir individualized drug detection kit and a method thereof. Background technique [0002] Abacavir (abacavir, referred to as ABC, trade name: Ziagen) is a new anti-AIDS drug. It is a new carbocyclic 2'-deoxyguanosine nucleoside drug. Its oral bioavailability is high and it can easily penetrate into the central nervous system. . Like other nucleoside reverse transcriptase inhibitors, abacavir is an inactive prodrug, which is metabolized into an active triphosphate through 4 steps in the body, and inhibits human immunodeficiency through the following 2 pathways The role of virus (HIV) reverse transcriptase: ① Competitively inhibit 2'-deoxyguanosine triphosphate (dGTP) (one of the DNA synthesis fragments) from incorporation into the nucleic acid chain; ② Effectively inhibit the addition of new bases Terminates DNA strand synthesis. [0003] Studies in rece...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6883C12N15/11
CPCC12Q1/6858C12Q2531/119C12Q2525/117
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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