Preparation and application for single-particle SPR probe used for real-time monitoring of G-quadruplex formation

A real-time monitoring, quadruplet technology, applied in the field of ion photonics with different appearance and size, can solve the application limitations and other problems, and achieve the effect of high sensitivity, wide detection range and fast detection speed.

Active Publication Date: 2016-01-13
NANJING UNIV OF POSTS & TELECOMM
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for detecting potassium ions, such as: fluorescence method, electrochemical method, colorimetric method, etc., but the probes based on these detection methods have great limitations in application due to size and sensitivity problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application for single-particle SPR probe used for real-time monitoring of G-quadruplex formation
  • Preparation and application for single-particle SPR probe used for real-time monitoring of G-quadruplex formation
  • Preparation and application for single-particle SPR probe used for real-time monitoring of G-quadruplex formation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 1 hour, rinse off excess surface modifier with ultrapure water to prepare a bioprobe for detecting potassium ions.

Embodiment 2

[0032] Preparation of SPR bio-optical probes with excellent performance:

[0033] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 2 hours, the excess surface modifier on the surface was washed away with ultrapure water to prepare a biological probe for detecting potassium ions.

Embodiment 3

[0035] Preparation of SPR bio-optical probes with excellent performance:

[0036] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 4 hours, rinse off excess surface modifier with ultrapure water to prepare a biological probe for detecting potassium ions.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides preparation and application for a single-particle SPR probe used for real-time monitoring of G-quadruplex formation. Based on good biological compatibility, large specific surface area and high SPR sensitivity of precious metal (e.g., gold and silver) nanoparticles, telomere Aptamer capable of specifically binding to potassium ions is used for surface modification of the precious metal nanoparticles, so the single-particle biological probe capable of sensitively detecting the potassium ions and monitoring G-quadruplex forming process in real time is simply and conveniently constructed. The whole specific bonding process can be characterized by the movement amount of SPR spectrum peaks of a single precious metal nanoparticle under the condition of combined usage of a dark-field microscopy (DFM) and a scattering spectrometer. The probe has a real-time detection function and advantages like a rapid detection speed, high sensitivity and a wide detection scope. In addition, a dissociation constant Kd and Gibbs free energy Delta G for formation of the G-quadruplex and two binding site forms in the forming process are obtained via fitting analysis.

Description

[0001] The invention specifically relates to a method capable of highly sensitively detecting potassium ions and monitoring the formation process of G-quadruplex in real time, belonging to the field of biological application of nanometer materials. Background technique [0002] When noble metal (gold, silver, etc.) nanoparticles are irradiated by incident light, free electrons and photons at the interface resonate and move to form a plasmon resonance effect. When the diameter of metal nanoparticles is larger than or close to the mean free path of free electrons, the excited particles will produce obvious scattering phenomenon. Since this phenomenon mainly occurs on the surface, it is called surface plasmon resonance (SPR) scattering. The study found that its scattering spectrum mainly depends on the shape and size of the nanoparticles and the dielectric constant of the particles and their surrounding environment. The difference in shape and size of nanoparticles will cause cha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/49
Inventor 张磊田媛媛汪联辉沈晶晶范曲立黄维
Owner NANJING UNIV OF POSTS & TELECOMM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap