Preparation and application for single-particle SPR probe used for real-time monitoring of G-quadruplex formation
A real-time monitoring, quadruplet technology, applied in the field of ion photonics with different appearance and size, can solve the application limitations and other problems, and achieve the effect of high sensitivity, wide detection range and fast detection speed.
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Embodiment 1
[0030] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 1 hour, rinse off excess surface modifier with ultrapure water to prepare a bioprobe for detecting potassium ions.
Embodiment 2
[0032] Preparation of SPR bio-optical probes with excellent performance:
[0033] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 2 hours, the excess surface modifier on the surface was washed away with ultrapure water to prepare a biological probe for detecting potassium ions.
Embodiment 3
[0035] Preparation of SPR bio-optical probes with excellent performance:
[0036] Soak the ITO glass in a solution of AuAg core-shell nanoparticles with a size of about 50 nm, and fix the AuAg core-shell nanoparticles on the ITO substrate by physical adsorption; then drop 200 μL of 1 nM Aptamer on the ITO glass and place it at 37 °C After 4 hours, rinse off excess surface modifier with ultrapure water to prepare a biological probe for detecting potassium ions.
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![application no application](https://static-eureka.patsnap.com/ssr/23.2.0/_nuxt/application.06fe782c.png)
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