Breeding method of high-photosynthetic-efficiency core collections of rape

A core germplasm and high-efficiency technology, applied in the fields of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of slow progress, difficult wide application, and high difficulty of high-light-efficiency breeding, and achieve favorable and sustainable development , avoid the effect of inaccurate results and accurate results

Inactive Publication Date: 2016-01-20
陕西省杂交油菜研究中心
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Problems solved by technology

[0004] The acquisition of high light efficiency germplasm is the top priority of high light efficiency breeding. In the prior art, the acquisition of high light efficiency germplasm of crops mainly adopts artificial mutagenesis (radiation, distant hybridization) to obtain variation, and adopts genetic engineering technology (transferred to PEPC , PPDK and other C 4 pathway photosynthetic enzymes), screening mutants using the "same room effect", improving plant type traits, activating or increasing C 3 C in crops 4 Methods such as pathway enzymes, the above existing methods pay too much attention to the improvement of single or a few photosynthetic physiological factors (such as photosynthetic rate, photosynthetic enzyme content or activity), only reflect the photosynthetic state of crops from a local perspective, and there are many factors for screening and comparison It is a photosynthetic physiological index, lacking the overall evaluation and comparison of materials; the data used are mostly the results measured in the laboratory, which are quite different from the real growth environment of crops, resulting in a large deviation between the experimental results and the actual situation
Therefore, although the high photosynthetic traits of the high photosynthetic germplasm screened are good, the economic yield is low, and the comprehensive traits such as quality and resistance are poor, so it is difficult to be widely used in production; It is quite difficult. Therefore, after nearly 50 years of hard work, only two crops, soybean and rice, have achieved initial success in breeding, and the breeding of other major grain and oil crops with high light efficiency has progressed slowly.

Method used

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  • Breeding method of high-photosynthetic-efficiency core collections of rape
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  • Breeding method of high-photosynthetic-efficiency core collections of rape

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Example 1: Breeding process of rapeseed high light efficiency core germplasm "7792-95 / 772 / / 772"

[0042] 1. 2006: Leaf chlorophyll and net photosynthetic rate were measured on the number 06GH118 (quoted from Dawangnan 17 male parent (04Sn254) genotype 7792-95 / 772 / / 772 in 2005). The measurement method is: continuously measure 14 individual plants, each individual plant measures the short-stalked leaf with the inverted 2 leaf position, and adopts the method of material rotation to reduce the difference in results caused by different measurement times; each material measures 2 pieces each time For the leaves, measure the materials used once within 30 minutes, a total of 7 times. The 6400-02BLED red and blue light source leaf chamber was used for the measurement, and the temperature in the leaf chamber was controlled at 25°C (TBlock), CO 2 The concentration is 400μmol / mL, and the light is 1100μmolm -2 ·s -1 , match at least 2 times every 30 minutes, and take the average ...

Embodiment 2

[0050] Example 2: Application of high light efficiency core germplasm "7792-95 / 772 / / 772"

[0051] 1. Transplantation of high-efficiency CMS CMS lines: Since 2012, it has been tested and found to be able to maintain, that is, the transfer of CMS lines has begun. Currently, 3 lines have been bred to the 4th generation;

[0052] 2. Application of chemical hybridization technology to prepare (CIMS) chemically induced hybrid combinations: 45 hybrid combinations were prepared in Zhangye Spring Sowing Base in Gansu Province in 2012, and in Dali Net Room in 2013 and 2014, and participated in the preliminary test.

[0053]3. The effect of directional selection on rapeseed high light efficiency core germplasm "7792-95 / 772 / / 772", see Table 1, Table 2 and figure 2 .

[0054] Table 1 - Breeding effect of main traits of "7792-95 / 772 / / 772" (comparison between 2014 and 2008)

[0055]

[0056]

[0057] Table 2- Effects of annual directional selection on chlorophyll content and net pho...

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Abstract

A breeding method of high-photosynthetic-efficiency core collections of rape comprises steps as follows: (1) screening of a high-photosynthetic-efficiency material of the rape; (2) screening of a high-photosynthetic-efficiency breeding nursery material; (3) rolling management of a high-photosynthetic-efficiency breeding nursery; (4) directional breeding of a high-photosynthetic-efficiency material; (5) hybrid improvement of the high-photosynthetic-efficiency material; (6) comprehensive identification of the high-photosynthetic-efficiency core collections. The breeding method has the characteristics that quantization and mechanical repetition can be realized, the non-transgenic technology is adopted and risks are avoided, increases the crop yield through improvement on the photosynthetic efficiency and has enormous yield increasing potential. With the adoption of the method, the requirement for screening the high-photosynthetic-efficiency collections of the rape can be met and the rape breeding method can be enriched and perfected; meanwhile, the method can provide beneficial reference for carrying out high-photosynthetic-efficiency breeding of other crops.

Description

technical field [0001] The invention relates to a method for breeding crops, in particular to a method for breeding high-light-efficiency core germplasm of rapeseed. Background technique [0002] my country's annual import of edible vegetable oil has reached more than 65%, which has seriously exceeded the international security warning line; and with the growth of population and the reduction of arable land, the oil crisis will become more and more serious. Rapeseed is the largest oil crop in my country and the fifth largest crop after rice, wheat, corn, and soybean. Rapeseed oil accounts for more than 50% of the self-produced vegetable oil. Rapeseed occupies an important position in my country's grain and oil production system. Therefore, the development of rapeseed production is of great significance to ensure the safety of edible oil in my country and promote the development of animal husbandry. However, the production level of rapeseed has been hovering at 3000-3750kg / hm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 张耀文赵小光田建华李殿荣赵兴忠王学芳陈文杰王竹云
Owner 陕西省杂交油菜研究中心
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