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A kind of small-scale Trichoderma solid-state fermentation method

A technology of solid-state fermentation and Trichoderma, applied in the direction of fermentation, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of autolysis, less sporulation, etc., and achieve good ventilation, less sporulation, and energy saving cost effect

Active Publication Date: 2018-08-17
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing small-scale fermented Trichoderma has less spore production and autolysis occurs in the later stage of fermentation, and provides a small-scale Trichoderma solid-state fermentation method

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  • A kind of small-scale Trichoderma solid-state fermentation method
  • A kind of small-scale Trichoderma solid-state fermentation method

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specific Embodiment approach 1

[0013] The specific embodiment one: the small-scale Trichoderma solid-state fermentation method of the present embodiment, carry out according to the following steps:

[0014] One, prepare PDA slant medium and inoculate Trichoderma, cultivate at room temperature for 4 to 6 days, then wash the Trichoderma conidia on the PDA slant medium with sterilized water, and prepare a concentration of 10 8 ~10 10 spores / mL of bacterial liquid;

[0015] Two, take by weight 80% of rice husk, 18% of wheat bran, 1% of cornmeal and 1% of soybean flour, add tap water to humidity of 180%, stir well and pack into a polypropylene sterilization bag, then Sterilize at 121°C for 2.5 hours to obtain sterilized fermented material;

[0016] 3. Put 150-160g of sterilized fermentation material in the sterile tray, add 1mL of the bacterial solution in step 1 and mix well, then seal it with plastic wrap, and cultivate it at room temperature under natural light until the Trichoderma mycelium is covered with...

specific Embodiment approach 2

[0027] Specific embodiment two: what this embodiment is different from specific embodiment one is, the preparation of PDA slant medium in step one: 200g potato, 20g glucose, 15g agar and 1000mL water, pack in the large polypropylene test tube, every tube 20mL , After sterilizing at 115°C for 30 minutes, place the inclined plane. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in step one, the Trichoderma is Trichoderma asperellum (Trichoderma asperellum) ACCC30536, Garner Trichoderma (Trichoderma ghanense) ACCC30153, Trichoderma harzianum (Trichoderma harzianum) ) ACCC30588, Trichoderma harzianum ACCC30371, Trichoderma harzianum ACCC31959, Trichoderma virens ACCC32490 or Trichoderma asperellum CFCC88162. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

[0029] In the present embodiment, Trichoderma asperellum (Trichoderma asperellum) ACCC30536 is purchased from the China Agricultural Microorganism Culture Collection Management Center; Ghana Trichoderma (Trichoderma ghanense) ACCC30153 is purchased from the China Agricultural Microbiology Culture Collection Management Center; Trichoderma harzianum (Trichoderma harzianum) ACCC30588, purchased from China Agricultural Microbiology Culture Collection Manag...

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Abstract

The invention provides a small-scale solid fermentation method for trichoderma and relates to a solid fermentation method. According to the small-scale solid fermentation method, the problems that the sporulation quantity is low, and an autolysis phenomenon occurs in the later period of the small-scale fermentation trichoderma are solved. The small-scale solid fermentation method comprises the following steps: (1) preparing a PDA slant culture medium, inoculating trichoderma, and preparing bacterial liquid after culture; (2) preparing a fermented feed, and carrying out sterilization; and (3) inoculating bacteria to the fermented feed to ferment, and finishing fermentation after all spores are dispersed. The small-scale solid fermentation method has the beneficial effects that production conditions do not need to be strictly controlled, the operation is simple, large-sized equipment is not required, an independent production space does not need to formed, small-scale production is suitably carried out in an laboratory, the occupied area is small, and the cost is low; meanwhile, the sporulation quantity of trichoderma is low, the fermentation cycle is short, the fermentation can be finished within only about 10 days, and the autolysis phenomenon does not occur in the later fermentation period.

Description

technical field [0001] The invention relates to a solid-state fermentation method. Background technique [0002] Trichoderma (Trichoderma) belongs to the subphylum Deuteromycota, Hyphospora, and the genus Trichoderma. It has three characteristics of "Trichoderma biological fertilizer", "Trichoderma biological pesticide" and "Trichoderma plant growth regulator". Countries all over the world have applied Trichoderma to agricultural production. Trichoderma grows rapidly, and sporulation requires a lot of oxygen and nutrients. Small-scale fermentation, poor control of conditions, often leads to fermentation failure. One is that the amount of spore production cannot meet the requirements, and the other is that autolysis often occurs in the late stage of fermentation, which seriously affects the effect of the bacterial fertilizer in the field test in the later stage. Contents of the invention [0003] The purpose of the present invention is to solve the problems of low spore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/885
Inventor 刘志华张荣沭王金杰周畅王玉成
Owner NORTHEAST FORESTRY UNIVERSITY
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