A kind of solid culture method of cicadae
A cultivation method, solid cultivation technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as energy waste, achieve the effects of reducing energy consumption, reducing spore production, and prolonging the service life
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Embodiment 1
[0015] The expanded cultivation of embodiment 1 cicadae
[0016] Slope culture: inoculate the strains into the inclined test tubes, then put the inclined test tubes inoculated with the strains into the incubator at 22°C, and cultivate for 7 days until the mycelium is full of the test tubes;
[0017] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;
[0018] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, and keep the pressure for 30-45 minutes;...
Embodiment 2
[0022] The solid culture of embodiment 2 cicadae
[0023] Preparation of solid medium: After washing the wheat and controlling the water, add an appropriate amount of water, the weight ratio of which is wheat: water is 1:1.4, and mix well; after mixing well, put it into a culture container, and control the thickness of the medium at 4cm. Place the culture container filled with the culture medium in a sterilizer, and sterilize at 121°C for 30-50 minutes; after sterilization, move the culture container to the buffer room, cool naturally to below 24°C, and move it to the inoculation room.
[0024] Inoculation: prior to inoculation, the culture container was irradiated with ultraviolet light for 0.5h in a ultra-clean workbench or a class 100 laminar flow hood (down); The inoculated containers are placed in a culture room for cultivation.
[0025] Culture conditions: In the dark culture stage, the culture temperature is 22-24°C, and the relative humidity of the air is 70-85%. When...
Embodiment 3
[0026] The solid culture of embodiment 3 cicadae
[0027] The cultivation process is basically the same as that in Example 2, except that the illumination time is 16 hours.
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