Preparation method and application of Fe3O4@CA-beta-CD nano microspheres

A technology of nano-microspheres and fe3o4ca-, applied in the field of biomedicine, can solve the problems of obvious burst release phenomenon and low drug loading rate in drug release

Inactive Publication Date: 2016-02-03
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are some problems in the existing magnetic-targeted drug delivery syst

Method used

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  • Preparation method and application of Fe3O4@CA-beta-CD nano microspheres
  • Preparation method and application of Fe3O4@CA-beta-CD nano microspheres
  • Preparation method and application of Fe3O4@CA-beta-CD nano microspheres

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Experimental program
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Embodiment 1

[0029] Fe in this example 3 o 4 The preparation method of CA-β-CD nano microspheres, the steps are as follows:

[0030] (1) Surface-modified ferric oxide nanoparticles Fe of citric acid 3 o 4 Preparation of CA; with FeCl 3 ·6H 2 O, FeSO 4 ·7H 2 O was the iron source, citric acid was used as the stabilizer, and citric acid-modified Fe3O4 was synthesized by ultrasonic-assisted co-precipitation method. 3 o 4 CA nanoparticles, where Fe 3+ with Fe 2+ The molar ratio of the citric acid is 2:1, the consumption of citric acid is 80% of the iron source consumption by mass, the reaction time is 15min, the aging time during coprecipitation method preparation is 30min, and the aging temperature is 90°C;

[0031] (2) Fe 3 o 4 Preparation of CA-β-CD nanospheres: 1gFe 3 o 4 Add CA nanoparticles to 1.5mL distilled water, then add 2g β-cyclodextrin, stir evenly, β is dried in a vacuum oven at 110°C for 3 hours, and the dried samples are washed 3 to 5 times with absolute ethanol a...

Embodiment 2

[0038] Fe in this example 3 o 4 The preparation method of CA-β-CD nano microspheres, the steps are as follows:

[0039] (1) Surface-modified ferric oxide nanoparticles Fe of citric acid 3 o 4 Preparation of CA; with FeCl 3 ·6H 2 O, FeSO 4 ·7H 2 O was the iron source, citric acid was used as the stabilizer, and citric acid-modified Fe3O4 was synthesized by ultrasonic-assisted co-precipitation method. 3 o 4 CA nanoparticles, where Fe 3+ with Fe 2+ The molar ratio of the citric acid is 2:1, the consumption of citric acid is 50% of the iron source consumption by mass, the reaction time is 15min, the aging time during co-precipitation method preparation is 30min, and the aging temperature is 90°C;

[0040] (2) Fe 3 o 4 Preparation of CA-β-CD nanospheres: Fe 3 o 4 Add CA nanoparticles into distilled water, 1gFe 3 o 4 CA nanoparticles need 1mL of distilled water, then add 0.8g of β-cyclodextrin, stir evenly, and dry in a vacuum oven at 115°C for 2.5h. Fe is separated...

Embodiment 3

[0042] Fe in this example 3 o 4 The preparation method of CA-β-CD nano microspheres, the steps are as follows:

[0043] (1) Surface-modified ferric oxide nanoparticles Fe of citric acid 3 o 4 Preparation of CA; with FeCl 3 ·6H 2 O, FeSO 4 ·7H 2 O was the iron source, citric acid was used as the stabilizer, and citric acid-modified Fe3O4 was synthesized by ultrasonic-assisted co-precipitation method. 3 o 4 CA nanoparticles, where Fe 3+ with Fe 2+ The molar ratio of the citric acid is 2:1, the consumption of citric acid is 100% of the iron source consumption by mass, the reaction time is 15min, the aging time during coprecipitation method preparation is 30min, and the aging temperature is 90°C;

[0044] (2) Fe 3 o 4 Preparation of CA-β-CD nanospheres: 1gFe 3 o 4 Add CA nanoparticles to 2 mL of distilled water, then add 1.6 g of β-cyclodextrin, stir evenly, and dry in a vacuum oven at 105 °C for 3.5 hours. The dried samples are washed with absolute ethanol and deoxy...

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Abstract

The invention discloses a preparation method and application of Fe3O4@CA-beta-CD nano microspheres. By adopting an ultrasonic-assisted coprecipitation method, citric acid modified Fe3O4@CA nanoparticles are synthesized. The preparation method comprises the following steps: adding Fe3O4@CA into distilled water, thereby obtaining a Fe3O4@CA solution; adding beta-cyclodextrin into the Fe3O4@CA solution, uniformly stirring, drying, washing, and separating by using a strong magnet, thereby obtaining the Fe3O4@CA-beta-CD nano microspheres; performing ultrasonic dispersion on Fe3O4@CA-beta-CD nano microspheres in deoxidated distilled water, adding doxorubicin, performing ultrasonic treatment by using an ultrasonic cell cracker, separating, washing, and drying, thereby obtaining doxorubicin-supported ferroferric oxide nano microspheres Fe3O4@CA-beta-CD-DOX. The microspheres are slow-released in a phosphate buffer at the pH value of 7.4, the release time is 12 hours, and a slow release effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a Fe 3 o 4 The preparation method and application of CA-β-CD nano microspheres. Background technique [0002] Ferroferric oxide nanoparticles are a nano-magnetic material that is easy to synthesize and has surface functionalization. It has good biocompatibility, biodegradability, and low toxicity. It is widely used in biomedical engineering and other fields, such as gene transfection. , cell and protein separation, magnetic resonance imaging, drug targeting delivery system; approved by the US FDA for magnetic resonance contrast agents and targeted drug delivery carriers. At present, there are many methods for preparing Fe3O4 nanoparticles, typical ones include aqueous co-precipitation method, thermal decomposition method, hydrothermal / solvothermal method, and microemulsion method. Due to the large surface area of ​​ferroferric oxide nanoparticles, they are prone to agglome...

Claims

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Application Information

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IPC IPC(8): B01J13/02A61K9/52A61K31/704
Inventor 郑林萍柴云许光宇郭秀丽张普玉
Owner HENAN UNIVERSITY
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