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Antibacterial peptide and application thereof

An antibacterial peptide and antibacterial agent technology, applied to the antibacterial peptide and its application field, can solve the problems of restricting the popularization and application of antibacterial drugs, poor stability and high hemolysis rate, and achieve low hemolytic activity and cytotoxicity, good stability and strong antibacterial activity. Effect

Active Publication Date: 2016-02-03
迈托姆科技(中山)有限公司
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Problems solved by technology

[0003] However, some natural antimicrobial peptides have problems such as immunogenicity, low antibacterial activity, poor stability, strong cytotoxicity, and high hemolysis rate, which limit their popularization and application as antibacterial drugs.

Method used

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  • Antibacterial peptide and application thereof
  • Antibacterial peptide and application thereof
  • Antibacterial peptide and application thereof

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] The extracellular membrane-adjacent region of HIV-1 (Ac- 665 WASLWNWFNITNWLWYIK 683 -NH 2 ) has the property of membrane activation and this region contains HIV-1 broad-spectrum neutralizing antibody 4E10 and 2F5 epitopes. According to the mechanism of action of antimicrobial peptides and the characteristics of amino acid composition, the present invention adds a water-soluble tail containing three lysines to its C-terminus on the basis of the 4E10 epitope to increase the positive charge and water solubility of the polypeptide. And on this basis, a series of polypeptide transformations were carried out. There are seven antimicrobial peptides in this embodiment, and their primary structure and molecular weight are shown in Table 1, and the amino acid sequence of their primary structure is shown in Table 2.

[0022] Table 1 P...

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Abstract

The invention discloses antibacterial peptide, and belongs to the field of molecular biology. The antibacterial peptide has an amino acid sequence which is as shown in SEQ ID NO:2: Asn Trp Phe Ala Ile Thr Asn Trp Leu Trp Tyr Ile Lys Lys Lys Lys. The antibacterial peptide disclosed by the invention not only has an obvious inhibiting effect on gram negative bacteria, such as Escherichia coli, bacillus subtilis and staphylococcus epidermidis, and positive bacteria, but also has very low hemolytic activity and cytotoxicity, and the antibacterial peptide has good stability and high antibacterial activity, has an efficient broad-spectrum antibacterial effect and can be used as antibiotics substitutes.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an antibacterial peptide and its application. Background technique [0002] In recent years, due to the abuse of traditional antibiotics, the problem of drug resistance of pathogenic microorganisms has become more and more serious, posing a huge threat to human health, and it is urgent to develop new antibacterial drugs. To solve this problem, antimicrobial peptides have received increasing attention. Antimicrobial peptides are active polypeptide substances produced by organisms when resisting the invasion of foreign pathogenic microorganisms, and are an important part of the innate immune response of organisms. It is generally composed of 20-60 amino acids, with a molecular weight of about 40kDa. So far, more than 1500 kinds of antimicrobial peptides have been found in animals, plants, microorganisms and humans. Antimicrobial peptides have the advantages of low mol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04
CPCY02A50/30
Inventor 单亚明贺晓秋
Owner 迈托姆科技(中山)有限公司
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