Multi-cell immune preparation for treating tumors and preparation method of multi-cell immune preparation
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An immune preparation, multi-cell technology, applied in anti-tumor drugs, animal cells, vertebrate cells, etc., can solve problems such as the inability to guarantee the safety of reinfusion and the limitations of tumor treatment
Inactive Publication Date: 2016-02-03
天晴干细胞股份有限公司
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Problems solved by technology
[0005] The present invention aims to solve the problem that the existing immune cell preparations are relatively limited in the treatment of tumors
Method used
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specific Embodiment approach 1
[0060] Specific embodiment 1: The multicellular immune preparation for treating tumors in this embodiment includes immune cells, human serum albumin and compound electrolytes, and the immune cells include DC cells, CIK cells, NK cells, CD3AK cells and γδT cells; the immune cells The concentration is 1×10 7 cells / ml~5×10 7 cells / ml, the concentration of human serum albumin is 1-2g / 100ml.
specific Embodiment approach 2
[0061] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the concentration of immune cells is 2×10 7 cells / ml~4×10 7 cells / ml. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0062] Specific embodiment three: The preparation method of the multicellular immune preparation for treating tumors in this embodiment is carried out according to the following steps:
[0063] 1. Preparation of autologous plasma:
[0064] ①Collect adult peripheral blood, the anticoagulant is sodium citrate, transfer the collected blood to a centrifuge tube, and centrifuge to collect plasma;
[0065] ② Inactivate the collected plasma and bathe in water at 56°C for 30-50 minutes. After the water bath is completed, centrifuge to collect the supernatant, which is autologous plasma, ready for use;
[0066] 2. Isolation of PBMC from peripheral blood mononuclear cells:
[0067] ① Divide the Ficoll solution into centrifuge tubes and wait for use. Mix adult peripheral blood cells and compound electrolytes according to the volume ratio (1-2): 1 to obtain a diluted cell suspension. Add the diluted cell suspension To the centrifuge tube filled with Ficoll solution, the volume ratio of ...
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Abstract
The invention provides a multi-cell immune preparation for treating tumors and a preparation method of multi-cell immune preparation and relates to an immune preparation and a preparation method thereof. According to the multi-cell immune preparation and the preparation method, the problems that the treatment of existing immune cell preparations to the tumors is relatively limited, and the infusion safety cannot be guaranteed are solved. The multi-cell immune preparation comprises immune cells, human serum albumin and compound electrolyte. The preparation method comprises the following steps: (1) preparation of autologous plasma; (2) separation of peripheral blood mononuclear cells PBMC; (3) preparation of DC; (4) preparation of CIK; (5) preparation of NK cells; (6) preparation of CD3AK; (7) preparation of gamma delta T; (8) preparation of the multi-cell immune preparation. The multi-cell immune preparation has broad spectrum activity to the treatment of the tumors, the immune cells are derived from the body of a patient, and exogenous substances such as fetal calf serum, trophoblast cells and the like are not introduced, so that the infusion safety is guaranteed. The multi-cell immune preparation is applied to the field of tumor treatment.
Description
technical field [0001] The invention relates to an immune preparation and a preparation method thereof. Background technique [0002] Peripheral blood mononuclear cells (PBMC), that is, cells with a single nucleus in peripheral blood, including lymphocytes, dendritic cells and a small number of other cells (hematopoietic stem cells, etc.). Through the induction of specific factors, PBMC can differentiate into a variety of immune cells in vitro, such as cytokine-induced killer cells (cytokine-inducedkiller, CIK), dendritic cells (dendritic cells, DCs) and natural killer cells, which are widely used in biological therapy. (nature killer cell, NK) and other cells. [0003] Tumor biotherapy is an emerging tumor treatment mode with significant curative effect. This treatment method has significant curative effect and no toxic side effects. The therapy integrates biotechnology and biological preparations and other technical means. Immune cells are collected from the patient, cul...
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