A Method for Quickly Obtaining Protoplasts from Herba halophyta Leaf

A protoplast and halophyte technology, applied in the direction of plant cells, can solve the problems of weak halophyte research, achieve good results, eliminate waste, and achieve large yields
CN105316276BActive Publication Date: 2018-08-03GANSU AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GANSU AGRI UNIV
Publication Date
2018-08-03

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Abstract

The present invention relates to a kind of preparation and purification method of saline steppe protoplast, which comprises the following steps: 1. Planting of seedling; 2. Selection of blade; 3. Obtaining of protoplast; Then put it into the enzymolysis solution; 4. Purification of protoplasts, after enzymolysis, add an equal volume of washing solution, and finally use a pipette to draw 4-5ml of the supernatant, which is the purer protoplasts; 5. The mensuration of protoplast yield, calculate the protoplast yield that every gram of blade obtains according to adding how many blades in the enzyme solution, calculation result expresses with the number of protoplasts per gram of blade (piece / g). The present invention is simple and easy, and the obtained protoplasts have high purity, and the number can reach 8.4×104 / g, and can also avoid tissue culture pollution, and can be used for hybridization of somatic cells, various organelles (such as nuclei, chloroplasts, mitochondria, vacuoles, etc.) ) separation, the construction of bioreactors and the fusion breeding of protoplasts lay the foundation.
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Description

technical field

[0001] The invention relates to a method for preparing and purifying saline grassland protoplasts, in particular to a method for directly obtaining relatively pure protoplasts by enzymolyzing leaves. Background technique

[0002] Plant cell protoplast refers to the product of removing the cell wall. It is a real single cell, which can be applied to the fusion between distant parents, easy to introduce foreign genes, and also to separate various organelles (such as nucleus, chloroplast, mitochondria, vacuole, etc.) It is a good material and can provide technical support for the research of material transport and information exchange between cells. Halogeton glomeratus is an annual herbaceous dilute halophyte of the Chenopodiaceae genus Halogeratus, which is distributed in western Gansu, Qinghai, Xinjiang and Tibet. In recent years, it has been paid attention to because of its excellent drought resistance and salt tolerance. It contains a large number of genes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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