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Method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis

A technology of proteolysis and small molecular peptides, applied in the field of bioengineering, can solve the problems of high production safety and labor safety requirements, long production cycle, high production cost, etc., and achieve production and labor safety and reliability, short production cycle and easy control Effect

Active Publication Date: 2016-02-17
深圳知本康业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] Among the above five methods for preparing porcine brain polypeptides, methods (1), (2), and (3) have the disadvantages of using a large amount of organic solvents, high production costs, and high requirements for production safety and labor safety; and the production cycle is long, only The time to isolate cerebroproteins was 48 hours, 30 hours and 40 hours, respectively
Method (four) and method (five) only do not use organic solvents, but the production cycle is long, and it takes 3-4 days from feeding materials to producing products

Method used

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  • Method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis
  • Method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis

Examples

Experimental program
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Effect test

Embodiment 1

[0048] A method for preparing brain polypeptides and small brain molecule peptides by enzymatic hydrolysis of pig brain protein, comprising the following steps:

[0049] (1) Take out the fresh pig brain or the thawed frozen pig brain, wash the dirt, blood and impurities on the surface with pure water, and remove the fascia and blood vessels;

[0050] (2) After mixing the cleaned pig brain according to the ratio of raw materials and purified water at 1:0.8, use a colloid mill to grind the homogenate, adjust to the coarsest fineness during the initial grinding, and then adjust to the fineness after the tissue is basically broken. For finer fineness, grind and homogenize for 8 minutes / batch, then add pure water twice as much as the homogenate and continue homogenizing for 2 minutes, mix the homogenized brain plasma of each batch evenly, and adjust the pH value to 9.0 with NaOH solution ; The thickest fineness described is 80 orders; The thinner fineness described is 120 orders; ...

Embodiment 2

[0060] A method for preparing brain polypeptides and small brain molecule peptides by enzymatic hydrolysis of pig brain protein, comprising the following steps:

[0061] (1) Take out the fresh pig brain or the thawed frozen pig brain, wash the dirt, blood and impurities on the surface with pure water, and remove the fascia and blood vessels;

[0062] (2) After mixing the cleaned pig brain according to the ratio of raw materials and purified water at 1:1.0, use a colloid mill to grind the homogenate, adjust to the coarsest fineness during the initial grinding, and then adjust to the fineness after the tissue is basically broken. For finer fineness, grind and homogenize for 10 minutes / batch, then add pure water twice as much as the homogenate and continue homogenizing for 3 minutes, mix the homogenized brain plasma of each batch evenly, and adjust the pH value to 9.0 with NaOH solution ; The thickest fineness described is 80 orders; The thinner fineness described is 120 orders; ...

Embodiment 3

[0072] A method for preparing brain polypeptides and small brain molecule peptides by enzymatic hydrolysis of pig brain protein, comprising the following steps:

[0073] (1) Take out the fresh pig brain or the thawed frozen pig brain, wash the dirt, blood and impurities on the surface with pure water, and remove the fascia and blood vessels;

[0074] (2) After mixing the cleaned pig brain according to the ratio of raw materials and purified water at 1:0.9, use a colloid mill to grind the homogenate, adjust to the coarsest fineness during the initial grinding, and then adjust to the fineness after the tissue is basically broken. For finer fineness, grind and homogenize the cycle for 9 minutes / batch, then add pure water twice as much as the homogenate and continue homogenizing for 2.5 minutes, mix the homogenized brain plasma of each batch evenly, and adjust the pH value with NaOH solution to 9.0; the coarsest fineness mentioned is 80 mesh; the finer fineness described is 120 me...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a method for preparing brain polypeptide and brain small-molecule peptide by means of pig brain protein through enzymolysis. The method for preparing the brain polypeptide and the brain small-molecule peptide by means of the pig brain protein through enzymolysis comprises the following steps of raw material preprocessing, pulp grinding, microwave processing, centrifuging, pulp conditioning, microwave enzymolysis performing, precipitating, rough filtering, micro-filtering, ultra-filtering, nano-filtering, RO membrane concentrating and freeze drying. According to the method, a microwave is in coupling connection with a high-speed centrifuge to replace the method that the pig brain isolate protein is extracted through organic solvent, the technology is simple, the time is short, the efficiency is high, and the production and labor are safe and reliable; the brain isolate protein extraction and enzymolysis time is short, and therefore the production cycle is short; the method is simple in technology, easy to control and suitable for being adopted by factory mass production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing brain polypeptides and small brain molecule peptides by enzymatic hydrolysis of pig brain protein. Background technique [0002] The hydrolyzate of mammalian brain protein can treat some brain function diseases, which has been clinically proven. In the late 1970s, an Austrian pharmaceutical company named Ebewe developed cerebrolysin, which is clinically used to treat nervous system diseases, especially the treatment of Alzheimer's disease (AD, commonly known as Alzheimer's disease) , can significantly delay the progress of senile dementia, improve the symptoms of dementia, and also have a good effect on the treatment of stroke, concussion, brain injury, cerebral arteriosclerosis, and neonatal cerebral ischemic hypoxic encephalopathy. This cerebrolysin is a biological preparation made from pig brain protein through hydrolysis and further ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/30
Inventor 梁尚文梁满水余冰妮梁轶
Owner 深圳知本康业有限公司
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