Coal transportation dust suppressant prepared based on microorganisms and use method thereof
A dust suppressant and microorganism technology, applied in the field of interdisciplinary science, can solve the problems of inconvenience of automation, inconspicuous effect at the same time, and rare popularization and application, and achieves excellent environmental friendliness, obvious dust suppression effect, and dust suppression time. adjustable effects
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Embodiment 1
[0033] (1) Weigh 13g of sucrose, Na 2 HPO 4 12H 2 O4g, MgSO 4 0.7g, CaCO 3 1.5g, KCl0.3g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.5g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool down, and inoculate the wet cells of Bacillus colioids Put it into the cooling medium solution, culture it with shaking at 30°C, the shaking frequency is 170r / min, and the culture time is 24h, and then concentrate to 10 times the concentration of the original bacterial solution;
[0034] (2) Use soluble calcium salt solution, such as: CaCl 2 , CaNO 3 etc., the calcium ion concentration is 0.5mol / L;
[0035] (3) Evenly mix the concentrated bacterial solution of Bacillus colloidus and the soluble calcium salt solution to make a microbial dust suppressant, and the ratio of the bacterial solution of Bacillus colloidus and the soluble calcium salt solution is 1:10;
[0036] (4) Take c...
Embodiment 2
[0038] (1) Weigh 13g of sucrose, Na 2 HPO 4 12H 2 O4g, MgSO 4 0.7g, CaCO 3 1.5g, KCl0.3g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.5g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool down, and inoculate the wet cells of Bacillus colioids Put it into the cooling medium solution, culture it with shaking at 30°C, the shaking frequency is 170r / min, and the culture time is 24h, and then concentrate to 10 times the concentration of the original bacterial solution;
[0039] (2) Use soluble calcium salt solution, such as: CaCl 2 , CaNO 3 etc., the calcium ion concentration is 0.5mol / L;
[0040] (3) Evenly mix the concentrated bacterial solution of Bacillus colloidus and the soluble calcium salt solution to make a microbial dust suppressant, and the ratio of the bacterial solution of Bacillus colloidus and the soluble calcium salt solution is 1:10;
[0041] (4) Take c...
Embodiment 3
[0044] (1) Weigh 13g of sucrose, Na 2 HPO 4 12H 2 O4g, MgSO 4 0.7g, CaCO 3 1.5g, KCl0.3g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.5g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool down, and inoculate the wet cells of Bacillus colioids Put it into the cooling medium solution, culture it with shaking at 30°C, the shaking frequency is 170r / min, and the culture time is 24h, and then concentrate to 10 times the concentration of the original bacterial solution;
[0045] (2) Use soluble calcium salt solution, such as: CaCl 2 , CaNO 3 etc., the calcium ion concentration is 0.1mol / L; 0.2mol / L; 0.5mol / L; 1.0mol / L;
[0046] (3) Evenly mix the concentrated bacterial solution of Bacillus colloidus and the soluble calcium salt solution to make a microbial dust suppressant, and the ratio of the bacterial solution of Bacillus colloidus and the soluble calcium salt solution...
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