Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primers, kit and method for detecting drug resistance of Acinetobacter baumannii

A technology for Acinetobacter baumannii and drug resistance, which is applied in the field of genetic testing, can solve the problems of high requirements for experimental technicians, time-consuming, high cost of experiments, etc., and achieves the problem of long-term experiments and finished products, high sensitivity, and good repeatability Effect

Active Publication Date: 2020-09-18
CHONGQING MEDICAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Method 1 is the gold standard for bacterial susceptibility determination, which takes a long time and requires high experimental technicians, and is suitable for fine diagnosis of Acinetobacter baumannii drug susceptibility; Method 2 has lower requirements for laboratory personnel, but the cost of the experiment is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers, kit and method for detecting drug resistance of Acinetobacter baumannii
  • Primers, kit and method for detecting drug resistance of Acinetobacter baumannii
  • Primers, kit and method for detecting drug resistance of Acinetobacter baumannii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Whether ComM is inserted directly determines whether the A. baumannii strain has natural competent function.

[0047] The laboratory standard strain of Acinetobacter baumannii does not have the ability to form natural competence. In this example, radioactive isotope-labeled DNA was used to test the DNA uptake ability of different Acinetobacter baumannii strains, so as to evaluate their competence formation ability. The different Acinetobacter baumannii strains used were Acinetobacter baumannii ATCC 17978, Acinetobacter baumannii AYE and Acinetobacter baumannii A118. Competent cells of different Acinetobacter baumannii strains (10ml; 1-3×10 9 cells / ml) were incubated at 30°C in a mineral medium containing α- 35S-dATP-radiolabeled DNA40ng / ml, take 0.5ml sample each time, and detect the radioactivity of the cells. The result is as figure 1 shown.

[0048] From figure 1 It can be seen that it is found that the laboratory standard strain Acinetobacter baumannii ATCC 17...

Embodiment 2

[0051] The recombinant plasmid containing the complete ComM gene was transformed into ATCC17978 bacteria by electroporation to obtain the ATCC17978-pET-comM recombinant strain. In the same manner as in Example 1, the radioactivity of the recombinant strain was detected. The result is as figure 2 shown.

[0052] From figure 2 It can be seen that the ATCC17978-pET-comM recombinant strain obviously obtained the natural competent function.

Embodiment 3

[0054] Most of the clinically detected Acinetobacter baumannii are multiple drug resistant (MDR) or extensive drug resistant (XDR). Fluoroquinolones and aminoglycoside antibiotics; extensively drug-resistant strains refer to those resistant to the above three types of antibiotics, but also resistant to carbapenem antibiotics.

[0055] First of all, it is assumed that this part of multidrug-resistant and extensively drug-resistant Acinetobacter baumannii is due to its complete natural competence, and in the subsequent evolution, it acquires exogenous drug-resistant elements and develops into multidrug-resistant or extensively drug-resistant feature. According to whether Acinetobacter baumannii has natural sensitivity, to a large extent, it is determined by whether the ComM gene is complete or not. The present invention quickly judges whether the ComM gene of Acinetobacter baumannii isolated from clinical trials has been inserted and destroyed. Bacilli have multidrug resistance...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of gene detection, in particular to primers, a kit and a method for detecting drug resistance of acinetobacter baumannii. The primers for detecting the drug resistance of the acinetobacter baumannii comprises an upstream primer P1 and a downstream primer P2, and the base sequences of P1 and P2 are represented as SEQ ID No.1 and SEQ ID No.2. The kit for detecting the drug resistance of the acinetobacter baumannii contains P1 and P2. According to the method for detecting the drug resistance of the acinetobacter baumannii, whether a to-be-detected sample has multi-drug resistance or extensive drug resistance is judged according to detection of ComM gene integrity of the to-be-detected sample. The upstream primer P1 and a downstream primer P2 are designed according to a ComM gene sequence of the acinetobacter baumannii and have the advantages of high specificity, high sensitivity, good repeatability and the like, and then whether the to-be-detected sample has multi-drug resistance or extensive drug resistance is judged on the basis of the ComM gene integrity.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a primer, a kit and a method for detecting drug resistance of Acinetobacter baumannii. Background technique [0002] Acinetobacter baumannii has a strong ability to acquire drug resistance and clone transmission, and multi-drug resistance, extensive drug resistance, and all-drug resistance Acinetobacter baumannii are prevalent worldwide. According to the data of China CHINET Bacterial Resistance Surveillance Network in 2010, Acinetobacter baumannii accounted for 16.11% of clinically isolated Gram-negative bacteria in 14 teaching hospitals in 10 provinces and cities in my country, second only to Escherichia coli and Klebsiella pneumoniae bacteria, has become one of the most important pathogens of nosocomial infection in my country. In addition, Acinetobacter baumannii has long-term survival ability in vitro, which is easy to cause clone dissemination. Therefore, the prevention and c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689
Inventor 龙泉鑫廖璞张震杜艳芬
Owner CHONGQING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com