Nano particles and application thereof

A nanoparticle and particle technology that is applied in the fields of analysis, measurement devices, and material analysis by optical means through chemical reactions of materials

Inactive Publication Date: 2016-03-02
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The combination of increasingly mature nanotechnology and clinical medicine provides some corresponding detection methods, but due to many factors such as price, it is difficult to be used as a routine method in clinical practice.

Method used

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  • Nano particles and application thereof
  • Nano particles and application thereof
  • Nano particles and application thereof

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preparation example Construction

[0031] The embodiment of the present invention discloses a method for preparing nanoparticles, comprising:

[0032] Mixing Ag / Au bimetallic nanoparticles, buffer solution, positively charged polymer and glucose oxidase solution, and standing still to obtain nanoparticles.

[0033] The present invention selects hollow Ag / Au bimetallic nanoparticles (Ag / AuNSs) as a base material for immobilizing glucose oxidase (GOx), and successfully combines the enzyme-catalyzed reaction with the plasmonic processing of the Ag / Au bimetallic nanostructure.

[0034] The preparation process of the nanoparticles of the present application, that is, the enzyme hybrid Ag / Au-GOxNSs is as follows:

[0035] Disperse the Ag / Au bimetallic nanoparticles (Ag / AuNSs) in the buffer solution, and adjust the pH value of the solution to 6-7, then add a positively charged polymer, add glucose oxidase after standing, let stand, Ag / Au-GOxNSs were obtained.

[0036]In the above process, the positively charged poly...

Embodiment

[0052] 1. Synthesis of Ag / Au bimetallic nanoparticles

[0053] First, the template colloidal silver nanoparticles protected by sodium citrate were synthesized, showing grayish yellow, and its surface plasmon resonance absorption peak was located at ~430nm; then the prepared nano-silver sol (nanoparticle concentration was ~1.5×10 11 / mL, 10mL) as the seed solution, add an equal volume (10mL) of deionized water and heat to boiling, then add NH 2 OH·HCl (0.02M, 0.1 mL) with 0.1% HAuCl in different volumes 4 , and stir. After the solution was heated to reflux for several minutes, it was cooled to room temperature and stored at 4°C until use.

[0054] figure 2 In order to add different amounts of 0.1% HAuCl to the AgNPs solution system during the synthesis process 4 Typical LSPR absorption spectra of three different Ag / AuNSs samples (Sample1-75 μl, Sample2-150 μl and Sample3-300 μl) (total size 50-60 nm) synthesized in aqueous solution and the amount of H 2 o 2 TEM images be...

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Abstract

The invention provides a preparation method of nano particles. The method comprises the steps that Ag / Au dual-metal nano particles, a buffer solution, a polymer with positive charges and a glucose oxidase solution are mixed for standing to obtain the nano particles. The glucose oxidase is fixed to the surface of the Ag / Au dual-metal nano particles through the electrostatic adsorption effect, and therefore the glucose oxidase and metal plasmon hybrid nano particles. The prepared nano particles can be used for glucose detection, and can also be used for preparing an early-stage cancer diagnosis and / or treating preparation.

Description

technical field [0001] The invention relates to the technical field of biotherapy, in particular to a nanoparticle and its application. Background technique [0002] Tumor is one of the chronic diseases threatening human life, its accurate detection and effective treatment has always been the focus of research in the field of biomedicine. Despite enormous efforts in recent years to find effective cancer diagnostics and treatments, cancer remains a major global public health challenge. The key to treating tumors is early diagnosis, and the cure rate of most early tumors is above 90%. Difficulty in early diagnosis and lack of effective treatment and prevention methods lead to high mortality of tumors. Clinically, the current diagnostic methods for tumors mainly include pathological diagnosis, X-ray, B-ultrasound, CT, and NMRI. Among them, pathological diagnosis is more accurate, but its accuracy is affected by the experience of medical workers, so it is easy to misdiagnose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/554G01N21/78
Inventor 金永东陈立梅贺海丽
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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