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An ionic liquid-ultrasonic-assisted-hplc-based method for the detection of active ingredients in Kunming mountain crabapple

An ultrasonic-assisted, ionic liquid technology, used in measurement devices, material separation, material analysis, etc., can solve problems such as difficulty in the detection of diversification of active components, and achieve the effect of avoiding detection errors.

Inactive Publication Date: 2017-11-10
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of the difficulty in detecting the diversity of active ingredients in Begonia Kunming, the present invention provides a method for detecting the active components of Begonia Kunming based on ionic liquid-ultrasound-assisted-HPLC, wherein the key technology is that the prepared samples are Using the solution of 1-butyl-3-methylimidazolium hexafluorophosphate as the extraction solution to extract Kunming mountain crabapple

Method used

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  • An ionic liquid-ultrasonic-assisted-hplc-based method for the detection of active ingredients in Kunming mountain crabapple
  • An ionic liquid-ultrasonic-assisted-hplc-based method for the detection of active ingredients in Kunming mountain crabapple
  • An ionic liquid-ultrasonic-assisted-hplc-based method for the detection of active ingredients in Kunming mountain crabapple

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Effect test

Embodiment 1

[0052] The condition investigation of embodiment 1 HPLC

[0053] 1. Instruments and reagents

[0054] Shimadzu HPLC LC-30A high-performance liquid chromatograph, AEG-45SM electronic balance (1 / 100,000, Shimadzu Corporation of Japan), BP121S (1 / 10,000) (Beijing Sartorius Scientific Instrument Co., Ltd.). KQ250DB CNC ultrasonic cleaner (power 250W, frequency 40kHz, Gongyi Yuhua Instrument Co., Ltd.), BJ-100 multifunctional pulverizer (Deqing Baijie Electric Co., Ltd.),

[0055] 1-Butyl-3-methylimidazolium bromide ([BMIm]Br, purity ≥99%), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm]BF 4 , purity ≥ 99%), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF 6 , purity ≥ 99%), 1-hexyl-3-methylimidazolium tetrafluoroborate ([HMIm]BF 4 , purity ≥99%), 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIm]PF 4, purity ≥ 99%), 1-octyl-3-methylimidazolium tetrafluoroborate ([OMIm] BF4, purity ≥ 99%), all provided by Lanzhou Institute of Chemical Physics, Chinese Academ...

Embodiment 2

[0064] Embodiment 2 The investigation of preparation sample condition

[0065] 1. Investigation of the types of ionic liquids in the extraction conditions

[0066] Take 0.5g each of 7 portions of Kunming Begonia powder (passed through a No. 3 sieve), weigh them accurately, and add 0.6mol·L -1[15] Six ionic liquids: 1-butyl-3-methylimidazolium bromide ([BMIm]Br), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm]BF 4 ), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF 6 ), 1-hexyl-3-methylimidazolium tetrafluoroborate ([HMIm]BF 4 ), 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIm]PF 6 ), 1-octyl-3-methylimidazolium tetrafluoroborate ([OMIm]BF4)﹜ methanol solution and pure methanol solvent 2mL each, ultrasonic extraction for 30min, centrifugation for 5min (12000r·min -1 ), filtered through a microporous membrane, injected into a high-performance liquid chromatograph, recorded the peak area, calculated the extraction rate of each component, investigated the...

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Abstract

The present invention provides a method for detecting the active components of Kunming mountain crabapple based on ionic liquid-ultrasound-assisted-HPLC, wherein the technical key is that the prepared sample is prepared by using 1-butyl-3-methylimidazole hexafluorophosphate The solution is used as an extract to extract Kunming mountain crabapple medicinal materials. The detection efficiency of the method provided by the invention is significantly higher than that of the traditional single-component abbreviation method, and more importantly, the transfer rate of the active ingredient in the process provided by the invention is significantly higher than that of the traditional methanol extraction method, which is effective and avoids detection errors.

Description

technical field [0001] The invention relates to a chromatographic detection method of Chinese medicinal materials, and more specifically relates to a detection method of Kunming mountain crabapple active components based on ionic liquid-ultrasound-assisted-HPLC. technical background [0002] Kunming Begonia is the dry root of Tripterygium hypoglaucum (Levl.) Hutch., a plant of the Euonymus family Tripterygium. It is pungent and bitter in taste, slightly warm, and highly toxic. effect. Also known as torch flower, heartbroken grass, purple gold skin, etc., the name of torch flower was first recorded in the "Compendium of Materia Medica". The raw material of "Begonia Tablets" is also a medicinal plant of Tripterygium wilfordii with high efficacy and low toxicity to humans and animals. It has a wide range of applications and has anti-inflammatory, immunosuppressive, anti-tumor, and kidney function improvement functions. It has been used for insecticide since ancient times. , r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/14
Inventor 陈一龙张小梅励娜姚媛媛梁旭明杨大坚张毅祝卢艺
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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