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Detection method of extract of seed of Mucuna macrocarpa Wall

A detection method and extract technology, applied in the detection field of quinoa bean extract, can solve problems such as harsh analysis conditions, difficult separation, lack of content analysis methods, etc.

Active Publication Date: 2016-03-02
苏州必益必诚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such components are highly polar and difficult to separate, and there is currently a lack of effective content analysis methods
The present invention has tried to use a hydrophilic interaction chromatographic column to analyze such components in the quinoa bean extract. The separation between the components is good, but the high-content LD chromatographic peaks are prone to overload, deformation, etc., and the mobile phase requires a high concentration. The acetonitrile organic phase is relatively harsh (Tan Beibei et al., Determination of the content of levodopa and its derivatives in cat beans by hydrophilic interaction chromatography, Suzhou University Journal of Medicine, 2012, 32: 593-506)

Method used

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  • Detection method of extract of seed of Mucuna macrocarpa Wall
  • Detection method of extract of seed of Mucuna macrocarpa Wall
  • Detection method of extract of seed of Mucuna macrocarpa Wall

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Embodiment 1

[0018] The preparation of embodiment 1 need testing solution

[0019] 3-Carboxyl-tetrahydroisoquinoline components 1-3 and LD have poor solubility in conventional solvents such as water and ethanol, and are easily oxidized. The content gap between LD and other components is large, so the preparation method of the original medicinal material for testing more critical. The present invention selects acid solution ultrasonic extraction for use, by extracting acid concentration (0.02-0.2mol L -1 [H + ]), the amount of extraction solvent (10, 20, 30 times), the extraction time (30, 60, 90min) were investigated and optimized, and finally the optimal preparation method of the original medicinal material for the test was established, and the corresponding quinoa bean extract for the test The preparation method of product solution.

[0020] Maodou MD-1 (20091101, white seed coat, Baise, Guangxi) and Maodou MD-2 (20111212, black seed coat, Nanning, Guangxi) are the dried seeds of Mucu...

Embodiment 2

[0023] The HPLC detection of embodiment 2 need testing product

[0024] Components 1-3 are characteristic components in quinoa bean, which can be used as quality control indicators of quinoa bean extract. Such components are similar in structure to LD and have strong polarity. They are hardly retained on conventional C18 columns, and the resolution is not good. After further research, the present invention, through comparison of various chromatographic columns, finally selects hydrophilic C18 chromatographic column and methanol solution (0-5%; more preferably 0-3%) as mobile equal degree elution, combined with modification Agent (0.5-1.5% acetic acid, 5-10mmol·L -1 Ammonium acetate, adjust pH2-4) and elution flow rate (0.5, 0.8, 1.0mL·min -1 ) optimization, the following chromatographic conditions were finally selected, components 1-3 and LD all obtained ideal baseline separation, thereby establishing a simultaneous quantitative analysis method for such components in the qui...

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Abstract

The invention relates to a detection method of extract of a seed of Mucuna macrocarpa Wall. The detection method comprises quantitative analysis of three 3-carboxyl-tetrahydroisoquinoline derivatives in extract of the seed of Mucuna macrocarpa Wall, and simultaneous quantitative analysis of levodopa. Concretely, the method comprises carrying out isocratic elution through a hydrophilic C18 chromatographic column and mobile phases such as a low concentration organic phase and an appropriate modifier (with pH of 2-4), and detecting desired component content by high performance liquid chromatography. Through use of the characteristic 3-carboxyl-tetrahydroisoquinoline derivatives of extract of the seed of Mucuna macrocarpa Wall as contrasts, the component quantitative analysis method is creatively built. The detection method has good specificity, high sensitivity and a scientific process, is simple and easy, and is suitable for simultaneous detection and quality control of 3-carboxyl-tetrahydroisoquinoline derivatives and levodopa of the extract of the seed of Mucuna macrocarpa Wall.

Description

technical field [0001] The invention relates to a detection method of a quinoa bean extract, and also relates to the application of the detection method in quality control of the quinoa bean extract. technical background [0002] Quinoa plants are distributed in tropical and subtropical regions of the world. In Indian Ayurvedic traditional medicine, quinoa is widely used to treat Parkinson's disease, snake bites, male infertility and other diseases. Levodopa (LD) is the main effective Element. Cat bean is the dried seed of Chenopodium chinensis. It is mainly produced in Guangxi, Guizhou and other regions of my country. It has the effect of warming the middle and replenishing qi and treating back pain. It is also used as the main raw material for the production of natural LD. LD is the first-line drug for the treatment of Parkinson's disease, but long-term use can lead to motor dysfunction (dyskinesia). Traditional medical experience and modern pharmacological research data...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 刘江云邓霖芳郝丽莉杨世林
Owner 苏州必益必诚医药科技有限公司
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