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A kind of colistin sulfate industrial production fermentation method

A technology of colistin sulfate and fermentation method, which is applied in the field of colistin sulfate industrial production and fermentation, can solve the problems of inconvenient operation, high cost of bulk raw materials, high sugar consumption, etc., and achieve saving time for pH adjustment, shortening time for loading tanks, The effect of reducing sugar consumption

Active Publication Date: 2018-10-12
新疆上昵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a colistin sulfate industrial production fermentation method, which can overcome the problems of high bulk raw material cost, high sugar consumption, and inconvenient operation in the current process, and can increase the potency to 560,000-600,000 u / g, improve efficiency by 5-10%

Method used

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  • A kind of colistin sulfate industrial production fermentation method
  • A kind of colistin sulfate industrial production fermentation method

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Experimental program
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Effect test

Embodiment 1

[0039] Colistin sulfate industrial production fermentation method of the present invention is:

[0040] (1) Preparation of fermenter: 110 ton tank, 80m metering volume 3 ;

[0041](2) First put 3.5% corn flour and 0.01% amylase (Xingtai Xinhua Enzyme Preparation Factory, 2000u / g) into the fermenter according to the weight ratio, add water to make the volume about 60% of the expected volume, and heat up to 80-84 ℃, keep warm and liquefy for 15 minutes; then add potassium dihydrogen phosphate 0.03%, foam enemy 0.02%, soybean peptone 0.1%, peanut cake powder 2.3%, ammonium sulfate 1.92%, ferrous sulfate heptahydrate (industrial) 0.02%, soybean oil 1 % and glucoamylase 0.01% (Xingtai City Xinhua Enzyme Preparation Factory, 50000u / g), add water to supplement until the sum of the mass percentages of each component of the material is 100%, cool down to 60-62°C, and keep warm for saccharification for 15min;

[0042] (3) Carry out disinfection, the disinfection temperature is 121℃~12...

Embodiment 2

[0053] Colistin sulfate industrial production fermentation method of the present invention is:

[0054] (1) Preparation of fermenter: 110 ton tank, 80m metering volume 3 ;

[0055] (2) First put 3.5% corn flour and 0.02% amylase (Xingtai Xinhua Enzyme Preparation Factory, 2000u / g) into the fermenter according to the weight ratio, add water to make the volume about 60% of the expected volume, and heat up to 80-84 ℃, heat preservation and liquefaction for 15 minutes; then add 0.03% potassium dihydrogen phosphate, foam enemy 0.022%, soybean peptone 0.11%, peanut cake powder 2.25%, ammonium sulfate 1.90%, ferrous sulfate heptahydrate (industrial) 0.018%, soybean oil 1 % and glucoamylase 0.02% (Xingtai City Xinhua Enzyme Preparation Factory, 50000u / g), add water to supplement until the sum of the mass percentages of each component of the material is 100%, cool down to 60-62°C, and keep warm for saccharification for 15min;

[0056] (3) Carry out disinfection, the disinfection temp...

Embodiment 3

[0067] Colistin sulfate industrial production fermentation method of the present invention is:

[0068] (1) Preparation of fermenter: 110 ton tank, 80m metering volume 3 ;

[0069] (2) First put 3.5% corn flour and 0.01% amylase (Xingtai Xinhua Enzyme Preparation Factory, 2000u / g) into the fermenter according to the weight ratio, add water to make the volume about 60% of the expected volume, and heat up to 80-84 ℃, heat preservation and liquefaction for 15 minutes; then add 0.03% potassium dihydrogen phosphate, foam enemy 0.018%, soybean peptone 0.09%, peanut cake powder 2.28%, ammonium sulfate 2.0%, ferrous sulfate heptahydrate (industrial) 0.022%, soybean oil 1 % and glucoamylase 0.01% (Xingtai City Xinhua Enzyme Preparation Factory, 50000u / g), add water to supplement until the sum of the mass percentages of each component of the material is 100%, cool down to 60-62°C, and keep warm for saccharification for 15min;

[0070] (3) Carry out disinfection, the disinfection tempe...

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Abstract

The invention provides an industrial production fermentation method of colistin sulfate. According to the fermentation method, corn flour, peanut meal and compound enzyme are adopted as the main raw materials, and the compound enzyme selects and uses amylase and glucamylase. According to the industrial production fermentation method of the colistin sulfate, the corn flour is taken as the raw material, the cost of the raw material is greatly reduced, the glucose consumption in the fermentation process is less, operation is easy and convenient, and the titer is increased to 560,000-600,000 u / g.

Description

technical field [0001] The invention relates to a fermentation method for the industrial production of colistin sulfate. Background technique [0002] Colistin sulfate, namely polymyxin E, is a compound of polymyxin E1, E2 and E1-2 sulfate. English name: COLISTIN SULPHATE, molecular formula: C 55 h 104 N 16 o 13 2.5H 2 SO 4 , molecular weight: 1441. Physical and chemical properties: colistin sulfate is white or off-white powder; odorless; hygroscopic; easily soluble in water, benzyl alcohol, slightly soluble in methanol, insoluble in acetone and ether, its free base is slightly soluble in water, at pH3 .0 ~ 7.5 range is more stable. It hardly breaks when placed at 100°C for several hours, and completely decomposes at room temperature for 48 hours under strong acid and strong alkali. In a dry state, stored at low temperature and protected from light, the vitality can remain unchanged for many years, and colistin sulfate powder can be stored at room temperature for th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
CPCC12P21/02
Inventor 陈剑慧田歌姜剑文顾广州颜卫
Owner 新疆上昵生物科技有限公司