Method for extracting protein peptide from poecilobdella manillensis

A technology of Phnom Penh leech and protein peptide, which is applied in the field of protein peptide preparation, can solve the problems of certain risks in the control of pollution and safety, and achieve the effects of strong physiological function, high separation rate and protective activity.

Active Publication Date: 2016-03-09
深圳知本康业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these processes involves the utilization of a large amount of protein after the hirudin is extracted, and conventional methods use a large amount of solvents, and there are certain risks in the control of pollution and safety.

Method used

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  • Method for extracting protein peptide from poecilobdella manillensis

Examples

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Embodiment 1

[0043] A method for preparing protein polypeptide from Phnom Penh leech, comprising the steps of:

[0044] (1) Rinse the pollutants on the body surface of fresh leeches or thawed leeches with clean water first, then move them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 10 minutes; wash off the potassium permanganate solution with clean water after picking up, and then put Move it into a 5% sodium chloride solution, stir and soak for 10 minutes, pick it up, wash off the salt on its body surface with clean water, rinse once with deionized water, drain the water, and place it in a container;

[0045] (2) After disinfecting and sterilizing the body surface of the leeches, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water, mix well according to the weight of the leeches, and put them into a colloid mill for grinding. The grinding temperature is controlled at less than 50°C , to obtain leech slurry; the fineness of the lee...

Embodiment 2

[0059] A method for preparing protein polypeptide from Phnom Penh leech, comprising the steps of:

[0060] (1) Rinse the pollutants on the body surface of fresh leeches or thawed leeches with clean water first, then move them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 15 minutes; pick up and wash off the potassium permanganate solution with clean water, and then put Move it into a 5% sodium chloride solution, stir and soak for 10 minutes, pick it up, wash off the salt on its body surface with clean water, rinse once with deionized water, drain the water, and place it in a container;

[0061] (2) After disinfecting and sterilizing the body surface of the leeches, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water, mix well according to the weight of the leeches, and put them into a colloid mill for grinding. The grinding temperature is controlled at less than 50°C , to obtain the leech slurry; the fineness of the leec...

Embodiment 3

[0075] A method for preparing protein polypeptide from Phnom Penh leech, comprising the steps of:

[0076] (1) Rinse the pollutants on the body surface of fresh leeches or thawed leeches with clean water first, then move them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 12 minutes; pick up and wash off the potassium permanganate solution with clean water, and then put Move it into a 5% sodium chloride solution, stir and soak for 10 minutes, pick it up, wash off the salt on its body surface with clean water, rinse once with deionized water, drain the water, and place it in a container;

[0077] (2) After disinfecting and sterilizing the body surface of the leeches, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water, mix well according to the weight of the leeches, and put them into a colloid mill for grinding. The grinding temperature is controlled at less than 50°C , to obtain leech slurry; the fineness of the leech sl...

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Abstract

The invention belongs to the technical field of biological engineering, and specifically relates to a method for extracting protein peptide from poecilobdella manillensis. The method comprises the following steps: disinfection, grinding, coarse filtration, microfiltration, ultrafiltration, alkali dissolution, microwave treatment, centrifugation, precipitation, discoloring, enzymatic hydrolysis, precipitation, microfiltration, ultrafiltration, nano filtration, condensation, and spray drying. A physical method is used to replace a chemical method to separate acidic polypeptide so as to obtain a coarse product of acidic polypeptide; the coarse product can be used to produce Chinese patent medicine containing acidic polypeptide and purified acidic polypeptide, the separation rate is high, the activity can be well protected, no pollution is generated, and clean production is achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing protein peptides from Phnom Penh leeches. Background technique [0002] Leeches, also known as leeches, belong to the class Hirudozoa. There are no setae on the body surface, and there are suckers at the front and rear ends. The body is muscular and the body cavity is small. It is a highly specialized annelid. According to reports, there are more than 500 species of leeches in the world, which are divided into blood-sucking and non-blood-sucking categories according to feeding habits. Blood-sucking mountain leeches live in damp shrubs and grass in mountainous areas. In China, the largest individual is the wide-bodied golden leech, which is 30cm long and weighs 40g. Like Phnom Penh leeches, weighing up to 150g, the smallest individual is only 0.1g. [0003] According to their feeding habits, leeches can be divided into two categories: no...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30
Inventor 梁尚文梁满水林栋才曾志亮
Owner 深圳知本康业有限公司
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