Method for quickly preparing flavonoid glycoside from oil-tea meal

A technology of camellia oleifera meal and flavonoid glycosides, which is applied in the field of rapid preparation of flavonoid glycosides in camellia oleifera meal, can solve the problems of large amount of solvent, numerous steps, and limited conditions, and achieves strong repeatability of stability, small loss of active ingredients, and easy mastery Effect

Active Publication Date: 2016-03-30
信阳学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For the separation and purification of camellia oleifera flavonoids, repeated silica gel column chromatography, medium pressure column chromatography, preparative high-performance liquid chromatography, high-speed countercurrent chromatography separation and purification, etc. are used for separation and purification; Chen Hongxia et al. (Chen Hongxia, Wang Chengzhang, Ye Jianzhong, Zhou Hao. Separation and structural identification of flavonoid glycosides in Camellia oleifera cake [J]. Forest Products Chemistry and Industry, February 2011, Vol. Methanol was dissolved and frozen to remove insoluble matter, flavonoid glycosides were prepared by medium and low pressure chromatography, and flavonoid glycosides I and II were further prepared by high performance liquid phase; patent CN201010229218.6 discloses a medium pressure column for rapid separation of oil tea cakes A method for preparing flavonoid glycosides in meal, which uses medium-pressure column chromatography plus high-performance liquid chromatography to prepare two flavonoid glycoside monomers with a purity of more than 95%. The use of professional equipment such as medium-pressure columns, preparative high-performance liquid chromatography, limited conditions, many steps, strong professionalism, difficult technology to master, small separation volume, etc.

Method used

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  • Method for quickly preparing flavonoid glycoside from oil-tea meal
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  • Method for quickly preparing flavonoid glycoside from oil-tea meal

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Embodiment 1

[0035] A method for rapidly preparing flavonoid glycosides in camellia oleifera meal, comprising the following steps:

[0036] (1) Use ethanol to extract the total flavonoids of camellia oleifera meal; the volume ratio is 50-95% ethanol, the extraction temperature is 40-70°C, the extraction liquid-solid ratio is (5-15):1 (ml:g), and the extraction times are 1- 4 times, each extraction for 1-3 hours, the extraction is carried out under stirring conditions, the stirring rate is 100 rpm, the extract is concentrated under reduced pressure to recover ethanol, and the crude extract of flavonoids of camellia oleifera meal is obtained;

[0037] (2) Purification of macroporous adsorption tree: Add water to the crude extract of camellia oleifera dregs flavonoids obtained in step (1) to prepare a crude extract of flavonoids from camellia oleifera dregs with a concentration of 3-8 mg / mL, using weak polar macroporous adsorption resin model HZ816 , particle size 0.3-1.2, for dynamic adsorpt...

Embodiment 2

[0040] A method for rapidly preparing flavonoid glycosides in camellia oleifera meal, comprising the following steps:

[0041](1) The total flavonoids of camellia oleifera meal was extracted with ethanol; the volume ratio was 95% ethanol, the extraction temperature was 60°C, the extraction liquid-solid ratio was 10:1, the extraction times were 2 times, each extraction was 2 hours, and the extraction was carried out under stirring conditions. The stirring rate is 100 revolutions per minute, the extract is concentrated under reduced pressure to recover ethanol, and a crude extract of flavonoids from camellia oleifera meal is obtained;

[0042] (2) Purification of macroporous adsorption tree: Add water to the crude extract of flavonoids from camellia oleifera meal obtained in step (1) to prepare a crude extract of flavonoids from camellia oleifera meal with a concentration of 5mg / mL, using macroporous adsorption resin model HZ816, particle size 0.3- 1.2, carry out dynamic adsorpt...

Embodiment 3

[0045] A method for rapidly preparing flavonoid glycosides in camellia oleifera meal, comprising the following steps:

[0046] (1) Ethanol was used to extract the total flavonoids of camellia oleifera meal; the volume ratio was 95% ethanol, the extraction temperature was 60°C, the extraction liquid-solid ratio was 10:1, the extraction times were 2 times, each extraction was 1 hour, and the extraction was carried out under stirring conditions. The stirring rate is 100 revolutions per minute, the extract is concentrated under reduced pressure to recover ethanol, and a crude extract of flavonoids from camellia oleifera meal is obtained;

[0047] (2) Purification by macroporous adsorption tree: Add water to the crude extract of flavonoids from camellia oleifera meal obtained in step (1) to prepare a crude extract of flavonoids from camellia oleifera meal with a concentration of 5 mg / mL, and use macroporous adsorption resin for dynamic adsorption enrichment and purification, 25 Dyn...

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Abstract

The invention discloses a method for quickly preparing flavonoid glycoside from oil-tea meal. The method comprises the following steps of firstly obtaining total flavonoid crude extracts from the oil-tea meal through ethanol extraction, performing enrichment and purification operation by utilization of macroporous adsorption resin, performing low pressure column chromatography refinement, and separating flavonoid glycoside monomers from the oil-tea meal. The method disclosed by the invention has the advantages that large precision equipment is unnecessary, the operation steps are simple and easily mastered, and the effects of simplicity, strong stability, strong repeatability, high yield and low loss of effective components are achieved; active components, namely flavonoid monomers, can be effectively separated from the oil-tea meal, and an important evidence is provided for further scale-up experiment and industrialized production, and excellent raw materials are provided for the development of flavonoid glycoside drugs and healthcare products from the oil-tea meal.

Description

technical field [0001] The invention belongs to the technical field of functional component extraction, and in particular relates to a rapid preparation method of flavonoid glycosides in camellia oleifera meal. Background technique [0002] Flavonoids widely exist in the plant kingdom and are an important class of polyphenolic natural products and an important class of natural organic compounds produced during plant metabolism. Flavonoids have a variety of biological activities in the human body, including anti-oxidation, free radical scavenging, anti-virus, anti-inflammation, vasodilation and antibacterial effects, and have been a hot research and development topic at home and abroad for many years. The dregs of camellia oleifera seeds after oil extraction (commonly known as camellia oleifera) are rich in triterpenoid plant saponins-tea saponin, and also contain other biologically active substances such as flavonoids and tannins. There are still relatively few studies on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 高进勇余炎炎臧明丽党占国高永平李欢鲁有强
Owner 信阳学院
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