Method for quickly preparing flavonoid glycoside from oil-tea meal

Abstract

The invention discloses a method for quickly preparing flavonoid glycoside from oil-tea meal. The method comprises the following steps of firstly obtaining total flavonoid crude extracts from the oil-tea meal through ethanol extraction, performing enrichment and purification operation by utilization of macroporous adsorption resin, performing low pressure column chromatography refinement, and separating flavonoid glycoside monomers from the oil-tea meal. The method disclosed by the invention has the advantages that large precision equipment is unnecessary, the operation steps are simple and easily mastered, and the effects of simplicity, strong stability, strong repeatability, high yield and low loss of effective components are achieved; active components, namely flavonoid monomers, can be effectively separated from the oil-tea meal, and an important evidence is provided for further scale-up experiment and industrialized production, and excellent raw materials are provided for the development of flavonoid glycoside drugs and healthcare products from the oil-tea meal.

Description

technical field [0001] The invention belongs to the technical field of functional component extraction, and in particular relates to a rapid preparation method of flavonoid glycosides in camellia oleifera meal. Background technique [0002] Flavonoids widely exist in the plant kingdom and are an important class of polyphenolic natural products and an important class of natural organic compounds produced during plant metabolism. Flavonoids have a variety of biological activities in the human body, including anti-oxidation, free radical scavenging, anti-virus, anti-inflammation, vasodilation and antibacterial effects, and have been a hot research and development topic at home and abroad for many years. The dregs of camellia oleifera seeds after oil extraction (commonly known as camellia oleifera) are rich in triterpenoid plant saponins-tea saponin, and also contain other biologically active substances such as flavonoids and tannins. There are still relatively few studies on t...

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Problems solved by technology

[0003] For the separation and purification of camellia oleifera flavonoids, repeated silica gel column chromatography, medium pressure column chromatography, preparative high-performance liquid chromatography, high-speed countercurrent chromatography separation and purification, etc. are used for separation and purification; Chen Hongxia et al. (Chen Hongxia, Wang Chengzhang, Ye Jianzhong, Zhou Hao. Separation and structural identification of flavonoid glycosides in Camellia oleifera cake [J]. Forest Products Chemistry and Industry, February 2011, Vol. Methanol was dissolved and frozen to remove insoluble matter, flavonoid glycosides were prepared by medium and low pressure chromatography, and flavonoid glycosides I and II were further prepared by high performance liquid phase; patent CN201010229218.6 discloses a medium pressure column for rapid separation of oil tea cakes A method for preparing flavonoid glycosides in meal, which uses medium-pressure column chromatography plus high-performance liquid chromatography to prepare two flavonoid glycoside monomers with a purity of more than 95%. The use of professional equipment such as medium-pressure columns, preparative high-performance liquid chromatography, limited conditions, many steps, strong professionalism, difficult technology to master, small separation volume, etc.

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