A QTL molecular marker method related to rice thousand-grain weight under low-nitrogen conditions
A molecular marker, 1000-grain weight technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of limited successful application cases, etc., to facilitate cross-breeding and speed up the breeding process. Effect
Active Publication Date: 2019-04-16
SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI +1
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Problems solved by technology
The above-mentioned alleles affecting grain weight are mainly derived from natural variation. The location and cloning of these genes help us understand the genetic basis of rice grain weight formation, but there are still very limited cases of their successful application in breeding practice
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Embodiment 1
[0016] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified.
[0017] (1) Association localization of thousand-grain de novo gene
[0018] 1. Test materials
[0019] Using Huang Huazhan as the recurrent parent and 8 parents from different countries as donors to construct 8 hybrid combinations, after one generation of backcrossing, harvest the backcrossing self-crossing seeds to form BC 1 f 2 Generations of segregation populations, after multiple generations of self-crossing, finally obtained 497 copies of BC 1 f 5 Strain material was used for QTL mapping of tolerance to low nitrogen. Phenotyping trials were carried out in both the early and late seasons of 2013. In each season, all test materials were planted under two conditions of low nitrogen and normal cultivation, and their yield and related traits were investigated.
[0020] 2. DNA ext...
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A QTL molecular marker method related to rice thousand-grain weight under low-nitrogen conditions belongs to the fields of rice high-yield stress-resistant breeding and molecular genetics. Use different rice varieties as donors to cross with the same recurrent parent, and then backcross with the recurrent parent once and self-cross once to obtain a random population. The random population is treated under low nitrogen and normal nitrogen fertilization conditions, and the bin of 7388 SNPs are combined Genotype data mapped to a thousand-grain de novo gene qTGW2‑1 that was stably expressed under low nitrogen conditions. Validation experiments showed that the practical and economical marker CLN9 was closely linked with this gene. Applying the present invention to assisted selective breeding and aggregation breeding of rice thousand-grain weight under low-nitrogen stress conditions, the seedling stage genotype selection can be performed on the low-generation breeding population under low-nitrogen stress conditions, and the process of grain identification at the adult plant stage is omitted. Improve breeding efficiency and speed up the breeding process.
Description
technical field [0001] The invention relates to a QTL molecular marker method related to rice thousand-grain weight affected by nitrogen levels, and belongs to the fields of rice high-yield stress-resistant breeding and molecular genetics. The gene can be applied to rice breeding practice for high yield and low nitrogen tolerance by using molecular marker assisted selection technology. Background technique [0002] Relevant statistical data show that more than 70% of the paddy fields in my country are medium and low-yield fields with low nitrogen content, which seriously restricts the growth of rice yield. Applying a large amount of nitrogen fertilizer is the main measure for rice growers in my country to obtain high yields. However, excessive nitrogen fertilizer is often easily lost with surface runoff and rainwater erosion, causing serious pollution to the ecological environment caused by eutrophication of rivers and lakes. planting costs. Therefore, excavating genes with...
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IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 徐建龙黎志康陈凯密雪飞朱亚军徐琴孙勇
Owner SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI