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Method for simultaneously preparing leaf application and root application agricultural biocontrol agents and application of leaf application and root application agricultural biocontrol agents

An agricultural production and preparation technology, applied in the biological field, can solve problems such as the use of nematicides, no chemical pesticides, etc., and achieve the effects of saving equipment investment, reducing production costs, and reducing production sites

Active Publication Date: 2016-03-30
宁夏张裕龙谕酒庄有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control of plant parasitic nematodes usually relies on chemical nematicides, cultivation techniques, breeding resistant varieties and biological pesticides, but there are still no specific chemical pesticides, and due to potential environmental problems, involving human and animal health, the use of nematicides subject to great restrictions

Method used

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  • Method for simultaneously preparing leaf application and root application agricultural biocontrol agents and application of leaf application and root application agricultural biocontrol agents
  • Method for simultaneously preparing leaf application and root application agricultural biocontrol agents and application of leaf application and root application agricultural biocontrol agents
  • Method for simultaneously preparing leaf application and root application agricultural biocontrol agents and application of leaf application and root application agricultural biocontrol agents

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 Shake flask culture prepares foliar use and root application agricultural biocontrol preparation simultaneously

[0030] Pick one ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30°C and 180rpm for 32h until the bacteria become turbid to obtain the activated seed solution. Activation medium (g / L): peptone 3, yeast powder 1, glucose 10, powdered chitosan 2, potassium dihydrogen phosphate 1, magnesium sulfate 1, disodium hydrogen phosphate 1, pH7.0. Divide in a 500ml Erlenmeyer flask Pack 100ml of activated medium and sterilize at 121°C for 20min.

[0031] Use the preferred medium (g / L): glycerol 18, soybean peptone 25, MnSO 4 1. Powder chitosan (deacetylation degree above 90%) 20, crab shell powder 10, NaCl2, CaCl 2 1. MgSO 4 1. FeSO 4 0.5, pH5, 200ml fermentation medium per 500ml Erlenmeyer flask, sterilized at 121°C for 20min. According to the inoculum amount of 0.8% (v / v), the...

Embodiment 2

[0033] Example 2 Fermentation Tank (10L) Cultivation Simultaneously Prepares Agricultural Biocontrol Preparations for Foliage and Root Application

[0034] Activation medium (g / L): peptone 5, yeast powder 3, glucose 20, powdered chitosan 5, potassium dihydrogen phosphate 3, magnesium sulfate 2, disodium hydrogen phosphate 3, pH 7.0. Divide in a 500ml Erlenmeyer flask Pack 100ml of activated medium and sterilize at 121°C for 20min. Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 32°C and 180 rpm for 36 hours until the bacteria become turbid to obtain the activated seed solution.

[0035] Use the preferred medium (g / L): glycerol 30, soybean peptone 40, MnSO 4 3. Powder chitosan (over 90% degree of deacetylation) 30, crab shell powder 15, NaCl5, CaCl 2 3. MgSO 4 3. FeSO 4 1.5. Fill the fermentation medium with a ratio of 70%, and the liquid volume is 7L. After steam sterilization at 118°C for 2...

Embodiment 3

[0037] Example 3 Fermentation Tank (50L) Cultivation Simultaneously Prepares Agricultural Biocontrol Preparations for Foliage and Root Application

[0038] Activation medium (g / L): peptone 4, yeast powder 2, glucose 15, powdered chitosan 3, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 2, pH7.0. Divide in a 500ml Erlenmeyer flask Pack 100ml of activated medium and sterilize at 121°C for 20min. Pick one ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30° C. and 180 rpm for 34 hours until the bacteria become turbid to obtain the activated seed solution.

[0039] Use the preferred medium (g / L): glycerol 20, soybean peptone 30, MnSO 4 2. Powder chitosan (more than 90% degree of deacetylation) 25, crab shell powder 12, NaCl4, CaCl 2 2. MgSO 4 2. FeSO 4 1. Fill the fermentation medium with a proportion of 70%, and the liquid volume is 35L. After steam sterilization at...

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Abstract

The invention discloses a method for simultaneously preparing leaf application and root application agricultural biocontrol agents and an application of the leaf application and root application agricultural biocontrol agents. A product is acquired by liquid fermenting paecilomyces lilacinus YT08 with a preservation number of CGMCC No. 10026, after the fermentation, the fermenting liquid is subjected to the solid-liquid separation, a solid part containing thallus is directly used as the root application agricultural biocontrol agent, and the liquid part is used as the leaf application agricultural biocontrol agent. The preparation method is simple, and the production process is easy to control and suitable for the mass production of factories; and moreover, the leaf application and the root application agricultural biocontrol agents have a good inhibition effect respectively on phytopathogen and root-knot nematode.

Description

technical field [0001] The invention relates to a method for simultaneously preparing agricultural biocontrol preparations for foliar application and root application, and its application in biological control of crop pathogens and root-knot nematodes, belonging to the field of biotechnology. Background technique [0002] Plant nematode disease is one of the four major pathogenic organisms in the cultivation of crops, vegetables, fruit trees, forest trees, flowers and medicinal materials. Its harm is second only to fungi and surpasses bacteria and viruses. Plant parasitic nematodes cause about 100 billion US dollars of losses in world agricultural production every year. The most important nematodes in global agriculture are root-knot nematodes and cyst nematodes. The more serious nematodes in my country include root-knot nematodes, soybean cyst nematodes, and wheat grain nematodes. , sweet potato stem nematode, rice stem apex nematode, chestnut nematode, pine wood nematode, e...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N1/14A01N63/04A01P3/00A01P5/00C12R1/79
CPCA01N63/30C12N1/14C12P1/02C12N1/145C12R2001/79
Inventor 程仕伟解孝满张萍缪静刘丹李加文
Owner 宁夏张裕龙谕酒庄有限公司