Method for preparing patterned phospholipid membrane array on ITO (indium tin oxide) conductive glass

Abstract

The invention discloses a method for preparing a patterned phospholipid membrane array on ITO (indium tin oxide) conductive glass, and the method comprises the steps: (1), self-assembling of TODS on the surface of ITO: immersing the washed ITO into fresh TODS-methylbenzene solution, sealing the solution and placing the solution silently for 4-12 hours, taking out the solution, placing the solution into methylbenzene for ultrasonic cleaning for 5-10 min, washing the methylbenzene on the surface of ITO through ethyl alcohol, carrying out drying through nitrogen, and carrying out the sealed storage for later use; (2), the preparation of a patterned ITO substrate: placing a chromium film plate with a patterned lattice on the ITO glass decorated with a TODS self-assembly film, and irradiating the chromium film plate for 17-25 min; (3), dripping 50-200 microlitre of phosphatide vesicle solution on the surface of a patterned ITO electrode, placing the electrode silently for 30-60 min under the temperature 20-60 DEG C, and forming a pattern phosphatide film on the ITO electrode. The phosphatide film prepared through method can be represented through a luminescence microscope and an electrochemical method, can be used for the research of the two-dimensional film electrophoresis of charged species in the film, and achieves the gathering and separating of charged species in the phosphatide film.

Description

technical field [0001] The invention relates to a method for preparing a patterned phospholipid film array, in particular to a method for preparing a patterned phospholipid film array on indium tin oxide conductive glass (ITO). Background technique [0002] Biomembrane is a general term for cell membrane (plasma membrane) and various organelle membranes. It plays a very important role in the process of energy transfer, material transfer, information recognition and transfer. However, due to the complex structure and variety of biofilm itself, and the preparation involves complicated purification processes, artificially prepared lipid bilayers are generally used as biofilm models to simulate the basic characteristics of biofilms. Phospholipid membrane arrays have great application prospects in studying the physical and biological properties of biological membranes, membrane fluidity dynamics, and interactions between phospholipid-linked biomolecules. The substrates for prepa...

AI Technical Summary

Problems solved by technology

The substrates for preparing phospholipid membrane arrays are generally gold, silicon dioxide, etc. However, because gold substrates have a quenching effect on fluorescent groups, the prepared phospholipid membranes cannot be characterized by fluorescence microscopy; although the phospholipid membrane arrays prepared on silica substrates Can be characterized by fluorescence microscopy, but phospholipid membranes cannot be characterized by electrochemical techniques due to the non-conductive substrate

Therefore, the phospholipid membrane arrays prepared on the above substrates cannot be characterized by fluorescence microscopy and electrochemical techniques at the same time.

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