Alkali-enzymolysis method for extracting sodium chondroitin sulfate

A technology of chondroitin sulfate sodium and cartilage, which is applied in the field of biochemical pharmacy, can solve the problems of affecting the yield and quality, easy dissociation of amino groups, and long time consumption, so as to improve production efficiency, shorten production cycle and improve production quality Effect

Inactive Publication Date: 2016-04-06
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above concentrated extraction methods, although the yield can reach a very high level, there are still big problems in the production methods, such as the removal of acidic protein in the process of salt solution to adjust the pH value of 1-2, which takes a long time when the product volume is large. Long, the amino group in sodium chondroitin sulfate is easily dissociated, thus affecting the yield and quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Soaking: 100 kg of pretreated white and clean animal cartilage blocks are placed in a reaction kettle, and an appropriate amount of water is added, and soaked for 5 hours;

[0017] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage according to the weight of the cartilage at 1:1, and add alkali tablets according to 4% of the weight of the cartilage, and stir for 3 hours;

[0018] (3) Enzymolysis: adjust the pH of the above alkaline solution to 8.0, heat to 45°C, add porcine pancreas according to 12% of the cartilage weight, add sodium chloride, and keep warm at 45°C for 3 hours;

[0019] (4) Reduction: adjust the pH of the above enzymolysis solution to 6.5, heat to 65°C, add 1% sodium dithionite to the cartilage weight, stir for 15 minutes, let stand for 20 minutes, and then filter;

[0020] (5) Precipitation: cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°C at 20°C, and preci...

Embodiment 2

[0024] (1) Soaking: Put 100kg of pretreated white and clean animal cartilage in a reaction kettle, add an appropriate amount of water, and soak for 6 hours;

[0025] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage at a ratio of 1:1 to the weight of the cartilage, and add alkali tablets to 5% of the weight of the cartilage, and stir for 4 hours;

[0026] (3) Enzyme hydrolysis: adjust the pH of the above alkaline solution to 8.5, heat to 50°C, add porcine pancreas according to 15% of the cartilage weight, add sodium chloride, and keep warm at 50°C for 4 hours;

[0027] (4) Reduction: adjust the pH of the above enzymatic solution to 7.0, heat it to 65°C, add 1% sodium dithionite to the cartilage weight, stir for 15 minutes, let stand for 20 minutes and then filter;

[0028] (5) Precipitation: Cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°C at 20°C, and precipitate for 7 hours;

[0029]...

Embodiment 3

[0032] (1) Soaking: Put 100kg of pretreated white and clean animal cartilage in a reaction kettle, add an appropriate amount of water, and soak for 6 hours;

[0033] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage at a ratio of 1:1 to the weight of the cartilage, and add alkali tablets to 6% of the weight of the cartilage, and stir for 4 hours;

[0034] (3) Enzyme hydrolysis: adjust the pH of the above alkaline solution to 8.5, heat to 48°C, add porcine pancreas according to 14% of the cartilage weight, add sodium chloride, and keep warm at 50°C for 4 hours;

[0035] (4) Reduction: adjust the pH of the above enzymatic solution to 7.0, heat it to 65°C, add 1% sodium dithionite to the cartilage weight, stir for 15 minutes, let stand for 20 minutes and then filter;

[0036] (5) Precipitation: Cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°C at 20°C, and precipitate for 8 hours;

[0037]...

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PUM

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Abstract

The invention relates to a novel extraction method of sodium chondroitin sulfate, particularly sodium chondroitin sulfate extracted by alkali-enzymolysis. The method mainly solves the problems of complex process, high alkali / acid or salt consumption, higher production cost, long extraction period, low product quality stability, lower yield and the like in the current like product production technique. The method mainly comprises the following steps: by using animal cartilage as a raw material, carrying out alkaline hydrolysis and one-step enzymolysis, thereby extracting the sodium chondroitin sulfate from the animal cartilage. The product has the advantages of white color, loose powder, high yield (up to 30% or above) and high purity, and conforms to the quality standard for oral administration. The method has the characteristics of simple production procedure, short period, low cost and the like, is simple to operate and easy to implement, is helpful to relieving the environmental pollution, and is beneficial to environment friendliness.

Description

technical field [0001] The invention relates to the field of biochemical pharmacy, in particular to a production process of sodium chondroitin sulfate which is directly extracted from animal cartilage and has good effects on cardiovascular and cerebrovascular diseases. Background technique [0002] Sodium chondroitin sulfate, English name Chondroitinsulfatessodium, is an acidic mucopolysaccharide extracted from animal cartilage, mostly present in animal cartilage, laryngeal bone, nasal bone (41%), bovine, equine septum and trachea, and its molecular structure is β- A polysaccharide chain composed of repeating disaccharide units formed by D-glucuronic acid and N-acetylgalactosamine through β1→3 glycosidic bonds. [0003] Sodium chondroitin sulfate is mainly used in the treatment of rheumatism and rheumatoid diseases. It also has anticoagulant, anticancer, antithrombotic, and blood lipid-lowering effects. It can be used to treat symptoms such as headache, migraine, coronary he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069
Inventor 刘乃山陆玉梅迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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