Method for increasing yield of immune cells

A technology of immune cells and cells, applied in the field of biomedicine, can solve the problems of reducing resistance, complicated steps, secondary damage, etc., and achieve the effect of improving yield, increasing yield and maintaining activity.

Inactive Publication Date: 2016-04-06
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are different methods for the separation and expansion of blood immune cells, and most of the steps are complicated. It is also difficult to obtain a large number of blood immune cells. Collecting blood when the patient is sick will also cause secondary damage to the patient and reduce the body's immune system. Immune cells reduce resistance, so how to efficiently and simply separate more immune cells, preserve and expand blood collected when healthy has become a problem that must be solved for immune cell therapy. Immune cells from blood have been separated in the prior art However, during the isolation process of this type of immune cells, the protection of the cells during the cryopreservation process is limited, the cells are easily damaged during the cryopreservation process, and the number of expansion during the culture process is also very limited.

Method used

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  • Method for increasing yield of immune cells
  • Method for increasing yield of immune cells
  • Method for increasing yield of immune cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the separation of immune cell

[0036] 1) Collect human peripheral blood and dilute it with normal saline to prepare blood dilution solution, the volume ratio of peripheral blood and normal saline is 1:5;

[0037] 2) Add the Ficoll separation solution to the bottom of the sepmate centrifuge tube, slowly add the blood dilution solution in step 1) to the upper layer of the Ficoll separation solution, the volume ratio of the blood dilution solution to the Ficoll separation solution is 1:5, and centrifuge at 400g in a centrifuge After 30 minutes, peripheral blood immune cells were separated, washed and centrifuged twice to collect peripheral blood mononuclear cells.

Embodiment 2

[0038] Example 2, the separation of immune cells

[0039] 1) collecting human peripheral blood and diluting it with normal saline to prepare a blood diluent, the volume ratio of peripheral blood and normal saline is 5:1;

[0040] 2) Add the Ficoll separation solution to the bottom of the sepmate centrifuge tube, slowly add the blood dilution solution in step 1) to the upper layer of the Ficoll separation solution, the volume ratio of the blood dilution solution to the Ficoll separation solution is 5:1, and centrifuge at 700g in a centrifuge After 20 minutes, peripheral blood immune cells were separated, washed and centrifuged twice to collect peripheral blood mononuclear cells.

Embodiment 3

[0041] Example 3, the separation of immune cells

[0042] 1) Collect human peripheral blood and dilute it with normal saline to prepare blood dilution solution, the volume ratio of peripheral blood and normal saline is 1:1;

[0043] 2) Add the Ficoll separation solution to the bottom of the sepmate centrifuge tube, slowly add the blood dilution solution in step 1) to the upper layer of the Ficoll separation solution, the volume ratio of the blood dilution solution to the Ficoll separation solution is 1:1, and centrifuge at 1000g in a centrifuge After 10 minutes, peripheral blood immune cells were separated, washed and centrifuged twice to collect peripheral blood mononuclear cells.

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PUM

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Abstract

The invention relates to the technical field of biomedical science, in particular to a method for increasing the yield of immune cells. The method for increasing the yield of the immune cells comprises the steps of dilution of human peripheral blood and separation, frozen preservation, resuscitation and induction and amplification culture of the immune cells. By extracting and preserving healthy cells and conducting induction and amplification culture at a later stage, the yield of the immune cells is greatly increased; by means of a specific cell frozen preservation solution adopted in the frozen preservation process, the activity of the immune cells can be effectively maintained; laminarin is added in the induction and amplification culture process, the cells which are high in amplification times and higher in purity can be obtained, and therefore the yield of the immune cells is significantly increased.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for increasing the yield of immune cells. Background technique [0002] Immune cells have the characteristics of natural killing and self-protection, among which lymphocytes have the characteristics of immune regulation and self-replication, and play a key role in the treatment of viral, bacterial, and fungal infections. It has great clinical application value. Immune cell therapy currently mainly includes cytokine-induced killer cell (CIK) therapy, dendritic cell (DC) therapy, DC-CIK cell therapy, natural killer cell (NK) therapy, DC-T cell therapy, etc. [0003] The blood of healthy people is rich in immune cells. With aging, sub-health, and various diseases, the number of immune cells in the blood will obviously decline, and the ability to proliferate and differentiate will decrease; while allogeneic immune cells Transplantation may cause immune rejection in pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078C12N5/0784C12N5/0783A01N1/02
CPCA01N1/0221C12N5/0634C12N5/0636C12N5/0639C12N5/0646C12N2500/34C12N2500/84C12N2501/2302C12N2501/2315C12N2501/2321
Inventor 王一飞陈海佳葛啸虎李丽娟
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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