Solid phase extraction column integrated surface enhanced Raman detection chip

A surface-enhanced Raman and solid-phase extraction column technology, which is applied in Raman scattering, test sample preparation, material excitation analysis, etc., can solve the problems of inability to realize pre-treatment and detection, limitation of application and detection effect, and complex production and processing and other problems, to achieve the effect of simple design, low cost and simple processing

Active Publication Date: 2016-04-06
XIAMEN PUSHI NANO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microfluidic chips based on SPE technology all need to collect the eluent after SPE pretreatment before detection, and cannot realize the integration of pretreatment and detection. The microfluidic channel is micron (less than 1mm), and there The manufacturing process is complicated, the channel is easily blocked, and the above-mentioned mi

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  • Solid phase extraction column integrated surface enhanced Raman detection chip
  • Solid phase extraction column integrated surface enhanced Raman detection chip
  • Solid phase extraction column integrated surface enhanced Raman detection chip

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0025] Example 1

[0026] See figure 1 with figure 2 , The present invention is a chip for integrating solid phase extraction column and surface enhanced Raman detection and integration. The chip is formed by sealing the upper, middle and lower three layers of substrates and cover plates, and the upper layer is provided with a sample inlet 1 And the surface-enhanced Raman detection chip 2; the middle layer is provided with a microfluidic channel 7, a sample detection cell 6 and a sieve plate groove 3. The microfluidic channel 7 has a sample inlet 5 at one end and a sample outlet 8 at the other end. A dam 4 is provided between the sample outlet 8 and the sample detection pool 6, and two sieve trays 3 are provided between the sample inlet 5 and the sample exit 8. The sieve tray 3 is used to put the polymer sieve in To prevent the filler from being washed away by the fluid; the middle micro flow channel of the two sieve plate grooves 3 forms a filler filling channel. The bottom su...

Example Embodiment

[0038] Example 2

[0039] Detection steps:

[0040] 1) With the chip inlet facing upwards, use a syringe to take 1ml of orange juice beverage containing lemon yellow pigment and slowly inject it into the chip through the inlet. The waste liquid drips down through the outlet due to gravity and is blocked by the dam structure. It will not flow into the detection cell to prevent the surface-enhanced Raman detection substrate from being contaminated by sample waste.

[0041] 2) Slowly inject the washing liquid (1ml aqueous solution and 1ml ethanol solution) in sequence using a syringe to wash the solid phase extraction column and wash off the impurities on the solid phase extraction column;

[0042] 3) Turn the chip over, with the sample inlet facing down, use a syringe to inject the eluent (0.5ml, 1% ethanol ammonia solution) to elute the sample, and the eluent will flow over the dam structure and flow into the detection cell and surface enhanced Raman Substrate contact.

[0043] 4) Afte...

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Abstract

The invention discloses a solid phase extraction column integrated surface enhanced Raman detection chip. The chip comprises an upper substrate, a middle substrate and a lower substrate, the middle substrate is provided with a microfluidic channel (7), a sample detection cell (6) and sieve plate grooves (3), one end of the microfluidic channel (7) is provided with a sample inlet (5), the other end of the microfluidic channel (7) gets close to the sample detection cell (6), and a dam (4) and two sieve plate grooves (3) are arranged between the sample inlet and the sample detection cell; the upper substrate is provided with a sample introduction port (1), and the bottom of the upper substrate is provided with a surface enhanced Raman detection substrate (2), wherein the surface enhanced Raman detection substrate (2) aligns at the sample detection cell (6) of the middle substrate, and the sample introduction port (1) aligns at the sample inlet (5) of the microfluidic channel of the middle substrate; and the lower substrate is provided with a detection cell outlet (10), and the detection cell outlet (10) is positioned right below the sample detection ell and is connected with the sample outlet (8) of the middle substrate. The chip realizes online Raman detection and SPE operation integration of a sample to be detected.

Description

technical field [0001] The invention relates to a microfluidic chip and a surface-enhanced Raman detection chip, in particular to a chip for integrating a solid-phase extraction column and a surface-enhanced Raman detection. Background technique [0002] Solid phase extraction (SPE) technology is widely used in separation / purification, concentration or enrichment of trace analytes, removal of interfering substances, and improvement of analyte concentration and detection sensitivity. It is a commonly used and important sample pretreatment method. Using the microfluidic channel structure formed by chip microfabrication technology to complete SPE operation on the microfluidic platform has many advantages that conventional SPE technology cannot match, such as fast extraction speed, low sample / reagent consumption, and integration with other operating units. [0003] Raman spectroscopy is the most important spectroscopic technique for analyzing various molecular structures and ide...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/40
Inventor 陈启振曾勇明刘国坤
Owner XIAMEN PUSHI NANO TECH CO LTD
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