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Solid phase extraction column integrated surface enhanced Raman detection chip

A surface-enhanced Raman and solid-phase extraction column technology, which is applied in Raman scattering, test sample preparation, material excitation analysis, etc., can solve the problems of inability to realize pre-treatment and detection, limitation of application and detection effect, and complex production and processing and other problems, to achieve the effect of simple design, low cost and simple processing

Active Publication Date: 2016-04-06
XIAMEN PUSHI NANO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microfluidic chips based on SPE technology all need to collect the eluent after SPE pretreatment before detection, and cannot realize the integration of pretreatment and detection. The microfluidic channel is micron (less than 1mm), and there The manufacturing process is complicated, the channel is easily blocked, and the above-mentioned microfluidic chips are not integrated with the surface-enhanced Raman detection substrate, which also leads to the limitation of the application and detection effect of this type of chip
The application of the detection chip based on the surface-enhanced Raman principle is also limited because it does not integrate the SPE pre-processing operation unit.

Method used

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  • Solid phase extraction column integrated surface enhanced Raman detection chip
  • Solid phase extraction column integrated surface enhanced Raman detection chip
  • Solid phase extraction column integrated surface enhanced Raman detection chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] see figure 1 and figure 2 , the present invention is a chip for integrating solid-phase extraction column and surface-enhanced Raman detection. and a surface-enhanced Raman detection chip 2; the middle layer is provided with a microfluidic channel 7, a sample detection pool 6, and a sieve plate groove 3; one end of the microfluidic channel 7 is provided with a sample inlet 5, and the other end is provided with a sample outlet 8. A dam 4 is provided between the sample outlet 8 and the sample detection pool 6, and two sieve plate grooves 3 are established between the sample inlet 5 and the sample outlet 8, and the sieve plate grooves 3 are used to put polymer sieve plates into the polymer sieve plate. To prevent the filler from being washed away by the fluid; the microfluidic channel in the middle of the two sieve plate grooves 3 forms a filler filling channel. The bottom surface of the dam structure and the interface between the substrate (the interface between the mi...

Embodiment 2

[0039] Detection steps:

[0040] 1) With the sample inlet of the chip facing up, use a syringe to take 1ml of orange juice drink containing lemon yellow pigment, slowly inject it into the chip through the inlet, the waste liquid will drip down through the sample outlet due to gravity, and at the same time, due to the dam structure blocking It will not flow into the detection cell, preventing the surface-enhanced Raman detection substrate from being contaminated by sample waste.

[0041] 2) Slowly inject the washing solution (1ml aqueous solution and 1ml ethanol solution) into the solid phase extraction column sequentially using the syringe, and wash off the impurities on the solid phase extraction column;

[0042] 3) Turn the chip over, with the injection port facing down, use a syringe to inject eluent (0.5ml, 1% ethanol ammonia solution) to elute the sample, the eluent overflows the dam structure, flows into the detection cell and surface-enhanced Raman base contact.

[00...

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Abstract

The invention discloses a solid phase extraction column integrated surface enhanced Raman detection chip. The chip comprises an upper substrate, a middle substrate and a lower substrate, the middle substrate is provided with a microfluidic channel (7), a sample detection cell (6) and sieve plate grooves (3), one end of the microfluidic channel (7) is provided with a sample inlet (5), the other end of the microfluidic channel (7) gets close to the sample detection cell (6), and a dam (4) and two sieve plate grooves (3) are arranged between the sample inlet and the sample detection cell; the upper substrate is provided with a sample introduction port (1), and the bottom of the upper substrate is provided with a surface enhanced Raman detection substrate (2), wherein the surface enhanced Raman detection substrate (2) aligns at the sample detection cell (6) of the middle substrate, and the sample introduction port (1) aligns at the sample inlet (5) of the microfluidic channel of the middle substrate; and the lower substrate is provided with a detection cell outlet (10), and the detection cell outlet (10) is positioned right below the sample detection ell and is connected with the sample outlet (8) of the middle substrate. The chip realizes online Raman detection and SPE operation integration of a sample to be detected.

Description

technical field [0001] The invention relates to a microfluidic chip and a surface-enhanced Raman detection chip, in particular to a chip for integrating a solid-phase extraction column and a surface-enhanced Raman detection. Background technique [0002] Solid phase extraction (SPE) technology is widely used in separation / purification, concentration or enrichment of trace analytes, removal of interfering substances, and improvement of analyte concentration and detection sensitivity. It is a commonly used and important sample pretreatment method. Using the microfluidic channel structure formed by chip microfabrication technology to complete SPE operation on the microfluidic platform has many advantages that conventional SPE technology cannot match, such as fast extraction speed, low sample / reagent consumption, and integration with other operating units. [0003] Raman spectroscopy is the most important spectroscopic technique for analyzing various molecular structures and ide...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/40
Inventor 陈启振曾勇明刘国坤
Owner XIAMEN PUSHI NANO TECH CO LTD
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