Method for tissue culture taking lotus reproduction and rooting into account

A technology of proliferation medium and tissue culture, applied in the field of plant tissue culture, can solve the problems of not being able to achieve rapid reproduction, taking a long time, and high cost, achieving simple and easy implementation steps, saving time, cost, and cost. low effect

Active Publication Date: 2016-04-13
杭州市园林文物局灵隐管理处(杭州花圃) +1
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Problems solved by technology

In order to find a convenient, fast and low-cost method for rapid proliferation, the predecessors have done a lot of research, but at present, most of the culture media used in lotus tissue culture are single proliferation media or rooting media, and they are all multiplied first. After rooting, this multiplication method takes a long time and costs high, and cannot achieve the purpose of rapid reproduction

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  • Method for tissue culture taking lotus reproduction and rooting into account

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Embodiment 1

[0013] Embodiment 1: A kind of tissue culture method that takes into account lotus multiplication and rooting

[0014] 1) Induction culture of mature embryo lotus seed buds

[0015] Take fully mature seeds, take mature embryos as explants, rinse them with tap water, etch them with concentrated sulfuric acid for 4 hours, rinse them with running water for 0.5 hours, peel off the shells, soak them for 15-18.5 hours, soak the soaked lotus seeds with 1% sodium hypochlorite The solution was sterilized for 10 minutes, and the sterilized lotus seeds were washed 5 times with sterile water, and the embryos were taken out, and inoculated on the sterilized MS (Murashige and Skoog) solid medium with a pH value of 5.7 at a temperature of 25±2°C. Cultivate in a culture room with a photoperiod of 16 / 8h and a light intensity of 2400lux;

[0016] 2) Proliferation culture

[0017] After 4 weeks of primary culture, the aseptic seedlings with good growth conditions and no browning were selected ...

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Abstract

The invention relates to a method for tissue culture taking lotus reproduction and rooting into account and belongs to the technical field of plant tissue culture. The method comprises the steps of selecting full ripe seeds, taking mature embryos as explants, taking out the embryos after cleaning and sterilization, inoculating the embryos to a sterilized MS solid culture medium with the pH of 5.7 and perform cultivation in a culture room with temperature of 25+ / -2 DEG C, photoperiod of 16 / 8 hours and light intensity of 2400 lux. After the preliminary generation is cultivated for 4 weeks, aseptic seedlings with good growth conditions and free of browning are transferred into a reproduction culture medium to be cultivated, the MS culture medium is taken as a base culture medium by the reproduction culture medium, 6-BA with concentration of 0.3-3 mg / L, NAA with concentration of 0.3-1 mg / L, sucrose with concentration of 30 g / L and agar of 0.3% are added, and the pH value is 5.7. According to the method, roots are induced while bud reproduction can be performed quickly in the reproduction and cultivation process, so that a large amount of lotus tissue culture seedlings are obtained, the time for expanding propagation is shortened, and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method that takes both multiplication and rooting of lotus into account. Background technique [0002] The lotus (Nelumbonucifera) is a perennial aquatic herbaceous flower belonging to the family Nymphaeaceae, also known as lotus and water hibiscus. Environmental beautification, medicinal use, food, and foreign trade export all have great application and development potential. At present, my country's lotus still adopts the traditional propagation methods of dividing lotus roots and planting in ponds or jars in large areas. However, with the traditional method of breeding, the lotus has a low reproductive coefficient (1:10), the amount of seed used is large, and the production cost is high. Moreover, long-term use of seed lotus for asexual reproduction will cause the degradation of the species and the accumulation of viruses. Coupled wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 唐宇力诸葛菲张海珍钱萍范丽琨周虹杨丽丽臧巧路林新春
Owner 杭州市园林文物局灵隐管理处(杭州花圃)
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