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A kind of preparation method of fermented indica rice flour

The technology of an indica rice flour and a pulverizer is applied in the field of preparation of special indica rice flour for fermentation, which can solve the problems such as difficulty in microbial utilization, reduction in nutrient utilization rate, influence on the application range of indica rice flour, etc. low cost effect

Active Publication Date: 2020-04-28
HENAN ZHENXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary indica rice flour is simply crushed and processed. The sugar in indica rice accounts for about 75%, and the protein content only accounts for about 7%. Because the sugar and protein are very tightly combined, it is difficult to be used by microorganisms, thus reducing the utilization of nutrients. rate, which affects the application range of indica rice flour in the field of industrial fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take a certain quality of indica rice, grind it through a pulverizer and pass through a 60-mesh sieve to get indica rice flour; add the prepared indica rice flour and purified water into an enzymolysis tank at a mass ratio of 1:2, and mix evenly; Add alkaline protease (200,000 U / g) at a ratio of 0.03% by mass, and perform enzymatic hydrolysis at 50°C for 1 hour, and control the pH value to 8.0 during the enzymolysis process; add neutral protease (50,000 U / g) at a ratio of 0.02% by mass of indica rice flour U / g), hydrolyze at 45°C for 2 hours, and control the pH value to 6.5 during the enzymolysis; add trypsin (4000 U / g) according to the proportion of 0.04% of the mass of indica rice flour, and enzymatically digest at 35°C Decompose for 3 hours, control the pH value to 7.5 during the enzymolysis process, and obtain the enzymolysis feed liquid; heat the obtained enzymolysis feed liquid to 80°C to inactivate the enzyme; send the obtained enzymolysis feed liquid into the twi...

Embodiment 2

[0027] Take a certain quality of indica rice, pass it through a 70-mesh sieve after being pulverized by a pulverizer, and then get indica rice flour; add the prepared indica rice flour and purified water into an enzymolysis tank at a mass ratio of 1:3, and mix evenly; Alkaline protease (200000 U / g) was added at a ratio of 0.08% by mass, and the enzymatic hydrolysis was carried out at 55°C for 2 hours. U / g), hydrolyze at 48°C for 3 hours, and control the pH value to 7.0 during the enzymolysis; add trypsin (4000 U / g) according to the proportion of 0.09% of the mass of indica rice flour, and enzymolyze at 37°C Decompose for 4 hours, control the pH value to 8.0 during the enzymolysis process, and obtain the enzymolysis feed liquid; heat the obtained enzymolysis feed liquid to 82°C to inactivate the enzyme; send the obtained enzymolysis feed liquid into the twin-screw extruder through the conveying pipeline The extruder is used for extruding and puffing. The temperature of extrusio...

Embodiment 3

[0030] Take a certain quality of indica rice, grind it through a pulverizer and pass through a 80-mesh sieve to get indica rice flour; add the prepared indica rice flour and purified water into the enzymolysis tank at a mass ratio of 1:4, and mix evenly; Alkaline protease (200000 U / g) was added at a ratio of 0.13% by mass, and the enzymolysis was carried out at 60°C for 3 hours, and the pH value was controlled at 10.0 during the enzymolysis process; neutral protease (50000 U / g), hydrolyze at 50°C for 4 hours, and control the pH value to 7.5 during the process; add trypsin (4000 U / g) according to the proportion of 0.14% of the mass of indica rice flour, and enzymatically digest at 40°C Decompose for 5 hours, control the pH value to 8.5 during the enzymolysis process, and obtain the enzymolysis feed liquid; heat the obtained enzymolysis feed liquid to 85°C to inactivate the enzyme; send the obtained enzymolysis feed liquid into the twin-screw extruder through the conveying pipeli...

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PUM

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Abstract

The invention discloses a preparing method of special long-shaped rice flour. The long-shaped rice flour special for fermenting is prepared by steps of primary crushing, biological enzymolysis, extruding puffing, drying and fine crushing. The preparing method has the beneficial effects of simple process, convenient operation, simple equipment demands, easiness in automation and low manufacturing cost; by a biological enzymolysis and physical puffing technology, an active sugar substance is released, and long-shaped rice protein is decomposed into polypeptide, energy substances such as active polypeptide and protein polypeptide required by microorganism can be provided, and synthesized precusor substance and nutrient factors required in a fermenting process can be provided, thus reducing a fermenting cost, improving a fermenting level and improving economic effects.

Description

technical field [0001] The invention belongs to the field of industrial fermentation, and in particular relates to a preparation method of special indica rice flour for fermentation. Background technique [0002] At present, industrial fermentation, especially antibiotic fermentation, such as streptomycin, erythromycin, tetracycline, and macrolide antibiotics mainly use indica rice flour as the main carbon source of the medium. Ordinary indica rice flour is simply crushed and processed. The sugar in indica rice accounts for about 75%, and the protein content only accounts for about 7%. Because the sugar and protein are very tightly combined, it is difficult to be used by microorganisms, thus reducing the utilization of nutrients. The rate affects the application range of indica rice flour in the field of industrial fermentation. Therefore, it is an urgent problem to be solved in the current fermentation industry to use indica rice to produce a carbon source medium for indus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/00
CPCC12P1/00
Inventor 常艺海岳新建王因博蔺晓曼陈倚天范庆富
Owner HENAN ZHENXIN BIOTECH CO LTD