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Compound microorganism agent and application thereof

A technology for compounding microbial inoculants and bacterial suspensions, applied in the field of agricultural microorganisms, can solve the problems of slow effect of microbial inoculants, high pathogen resistance, and attenuation of the number of viable bacteria in microbial inoculants.

Inactive Publication Date: 2016-04-20
海南博士威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of plant diseases is still overly dependent on chemical agents for prevention and control, which not only has a harmful impact on the environment, but also has an increasing resistance to pathogens, and also affects product quality.
[0003] Aiming at the inadequacies of the prevention and control methods of chemical agents that treat the symptoms but not the root cause, many scientific research achievements in recent years have also shown that improving the immunity of plants can effectively prevent and control the occurrence and development of diseases, but most of the plant immune preparations currently on the market are It is fighting alone, and it cannot be combined with the needs of plant growth in an all-round way, so the effect is not very obvious
[0004] At present, many beneficial microbial agents are increasingly favored by agricultural producers, such as Bacillus subtilis, Streptomyces ochreus, etc., which can not only inhibit the growth of various pathogenic bacteria, protect plants from damage, but also provide plants with Some growth nutrients can improve the immunity of plants, but the effect of many microbial agents is relatively slow, and the number of live bacteria of microbial agents also has the effect of attenuation, which leads to the reduction of the activity of microbial agents

Method used

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  • Compound microorganism agent and application thereof
  • Compound microorganism agent and application thereof
  • Compound microorganism agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Antagonism test between Bacillus subtilis and Streptomyces ochreus

[0015] Prepare the bacterial suspensions of Streptomyces yellow ocher and Bacillus subtilis cultured on the slant, and use a sterile pipette gun to draw 100ul each of the suspensions of Streptomyces yellow ocher and Bacillus subtilis and apply them to Gaoshi Synthetic No. 1 and Bacillus subtilis respectively. On the nutrient gravy culture plate, after standing for a while to infiltrate, stick a sterile filter paper piece with a diameter of 5mm on the corresponding position of the culture plate, stick 3 pieces of filter paper on each culture plate, and then use a sterile pipette gun to absorb the yellow ochre. Add 10ul each of Streptomyces and Bacillus subtilis suspension to the corresponding filter paper respectively, absorb 10ul of Gaoshi Synthetic No. 1 and Nutrient Gravy Liquid Medium respectively and drop them on the corresponding filter paper as the test control group, let stand for a whi...

Embodiment 2

[0020] Example 2 Seaweed prolongs the number decay time test of viable bacterial strains

[0021] Make a bacterial suspension of Streptomyces yellow ochreus cultured on a slant, inoculate 10ml into 200ml Gaoshi Synthetic No. 1 liquid medium; make a bacterial suspension of Bacillus subtilis cultured on a slant, inoculate 10ml into 200ml nutrient gravy liquid medium ; Shake culture at 280r / min, 30°C for 24h, until the OD value of the bacterial suspension is 0.8-1.0, and the bacterial concentration is 1×10 9 -1.5×10 9 cfu / ml, according to the ratio of protective agent: strain = 1:4, add seaweed into Streptomyces yellow ochreus and Bacillus subtilis liquid culture solution respectively, and add sterile water in equal proportions as the test control group, The number of viable bacteria was measured after mixing and after 6 months, 12 months and 24 months. The test results are as follows:

[0022] Table 2 The test results of seaweed prolonging the decay time of the number of viab...

Embodiment 3

[0025] Example 3 Composite microbial bacterial agent control capsicum bacterial wilt test

[0026] Make the bacterial suspension of Streptomyces yellow ocher cultivated on the slant, inoculate 10ml into 200ml Gaoshi Synthetic No. 1 medium; make the bacterial suspension of Bacillus subtilis cultivated on the slant, inoculate 10ml into 200ml nutrient gravy liquid medium; Shake culture at 280r / min and 30°C for 24h until the OD value of the bacterial suspension is 0.8-1.0 and the bacterial concentration is 1×10 9 -1.5×10 9 For cfu / ml, compound according to the ratio of seaweed: Streptomyces yellow ocher: Bacillus subtilis = 2:4:4 to obtain the composite microbial agent of the present invention.

[0027] Before transplanting peppers, dilute the above-mentioned seaweed, Streptomyces yellow ochreus culture solution, Bacillus subtilis culture solution and compound bacterial agent by 500 times, irrigate with 150ml per plant and set up a blank control group; transplant for 3 weeks Aft...

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Abstract

The invention belongs to the technical field of agricultural microbiology, and discloses a compound microorganism agent and application thereof. The compound microorganism agent is formed by compounding a seaweed element, a yellow-ochre streptomyces fermentation culture solution and a bacillus subtilis fermentation culture solution in a ratio of (2-3):(3-5):(3-5), preferentially 2:4:4. The compound microorganism agent is capable of not only prolonging the decaying time of microorganism living bacteria, but also improving the prevention and control effect to plant diseases, and can reach prevention efficiency of 90% or above to various diseases.

Description

Technical field: [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a compound microbial bacterial agent for preventing and treating plant diseases. Background technique: [0002] Plant disease is the biggest evil cause of the current agricultural production reduction and product quality getting worse and worse, which greatly affects the sustainable and healthy development of agriculture in my country, and the effective prevention and control of plant diseases is also the biggest problem facing the national economic crop agricultural production. problem. At present, the prevention and control of plant diseases is still overly dependent on chemical agents for prevention and control, which not only has a harmful impact on the environment, but also has an increasing resistance to pathogens, and also affects product quality. [0003] Aiming at the inadequacies of the prevention and control methods of chemical agents that treat the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/22A01P1/00A01P3/00A01P21/00
CPCA01N63/00A01N25/22A01N2300/00
Inventor 吴景霄任承才杜荣锴
Owner 海南博士威生物科技有限公司
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