Biological agent used for enhancing immunity of organism and preparation method thereof
A technology of biological preparations and immunity, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, applications, etc., can solve the problems of difficult use of active ingredients, cumbersome and complicated process, and ineffective effects, etc., to achieve Easier absorption and utilization, significant effect, and immune-enhancing effect
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[0031] The invention provides a method for preparing a biological agent for enhancing body immunity, which is characterized in that it comprises:
[0032] Step 1: Mix according to the weight ratio of 10-20 parts of Saccharomyces cerevisiae, 20-30 parts of Lactobacillus plantarum and 35-50 parts of Bifidobacterium to obtain mixed bacteria, add them to brown sugar water medium, and store them at 32-35°C Cultivate under airtight conditions until the pH is less than 3.60, and then continue to cultivate for 12-15 days to obtain complex bacteria;
[0033] Step 2: mix according to the weight ratio of 20-30 parts of kudzu root, 15-25 parts of seabuckthorn, 10-15 parts of grape seed, 10-15 parts of brown sugar, and 40-60 parts of water to obtain a compound medium;
[0034] Step 3: adding the composite bacteria to the composite culture medium, and culturing in a sealed environment at 30-38°C for 20-25 days to obtain a composite fermentation product;
[0035] Step 4: Soaking, washing, c...
Embodiment 1
[0057] A method for preparing a biological preparation for enhancing body immunity, comprising:
[0058] The first step: cultivation of strains: insert the Saccharomyces cerevisiae strains (Saccharomycescerevisiae, ACCC20065) purchased from China Agricultural Microorganism Culture Collection and Management Center into the Erlenmeyer flask of potato liquid medium, shake at 28°C, 180-220rpm Cultivate for 48 hours, take out after the medium is turbid; put Lactobacillus plantarum (ACCC11118) and Bifidobacterium (ACCC11054) into the Erlenmeyer flask of MRS liquid medium respectively, at 37°C Cultivate under the same conditions for 48 hours, take out the medium after it becomes turbid, and then store it in a 4°C refrigerator for later use;
[0059] The second step: according to the weight ratio of 15 parts of Saccharomyces cerevisiae, 30 parts of Lactobacillus plantarum, and 45 parts of Bifidobacterium to obtain mixed bacteria, add it to the brown sugar water culture medium accordin...
Embodiment 2
[0065] A method for preparing a biological preparation for enhancing body immunity, comprising:
[0066] The first step: cultivation of strains: insert the Saccharomyces cerevisiae strains (Saccharomycescerevisiae, ACCC20065) purchased from China Agricultural Microorganism Culture Collection and Management Center into the Erlenmeyer flask of potato liquid medium, shake at 28°C, 180-220rpm Cultivate for 48 hours, take out after the medium is turbid; put Lactobacillus plantarum (ACCC11118) and Bifidobacterium (ACCC11054) into the Erlenmeyer flask of MRS liquid medium respectively, at 37°C Cultivate under the same conditions for 48 hours, take out the medium after it becomes turbid, and then store it in a 4°C refrigerator for later use;
[0067] The second step: according to the weight ratio of 10 parts of Saccharomyces cerevisiae, 30 parts of Lactobacillus plantarum and 50 parts of Bifidobacteria to obtain mixed bacteria, add it to brown sugar water culture medium according to t...
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