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Cadmium alginate, lead alginate and copper alginate nanoparticles and their preparation method and application in the preparation of electrochemical immunoprobes

A technology of lead alginate and copper alginate is applied in the field of immunoassay and detection to achieve the effects of wide detection range, good reproducibility and low detection limit

Inactive Publication Date: 2017-10-24
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The alginic acid microspheres prepared by these methods are all at the micron level. At present, the preparation of alginic acid microspheres with uniform particle size, good sphericity, and nano-scale is still a difficult problem to be solved.

Method used

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  • Cadmium alginate, lead alginate and copper alginate nanoparticles and their preparation method and application in the preparation of electrochemical immunoprobes
  • Cadmium alginate, lead alginate and copper alginate nanoparticles and their preparation method and application in the preparation of electrochemical immunoprobes
  • Cadmium alginate, lead alginate and copper alginate nanoparticles and their preparation method and application in the preparation of electrochemical immunoprobes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0039] (1) 2.0 g of triton, 1.0 g of n-hexanol, 3.0 g of n-octane, and 1.5 mL of 1.5% (w / w) sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0040] (2) Mix 2.0g triton, 1.0g n-hexanol, 3.0g n-octane, and 0.5M 1.5mL of CdCl 2 Aqueous solution, Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 45 minutes to prepare a uniform microemulsion 2.

[0041] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 4 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate or copper alginate nanospheres; transmission electron microscope The observed morphology of cadmium alginate, lead alginate and copper alginate nanospheres is as follows: figure 1 A, 1B and 1C are shown. The morphology of cadmium algi...

Embodiment 2

[0057] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0058] (1) 2.5 g of triton, 1.5 g of n-hexanol, 3.5 g of n-octane, and 2.0 mL of 1.5% (w / w) sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0059] (2) 2.5g triton, 1.5g n-hexanol, 3.5g n-octane, and 1.0M 2.0mL of CdCl 2 Aqueous solution, Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 1 hour to prepare a uniform microemulsion 2.

[0060] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 5 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate or copper alginate nanospheres.

[0061] 2. Preparation of immune probes and examples of their immune properties using cadmium alginate, lead alginate and copper alginate nanospheres:

[0062] 2.1 Tested sample: human serum

[0063] 2.2 Metho...

Embodiment 3

[0076] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0077] (1) 1.5 g of triton, 0.5 g of n-hexanol, 2.5 g of n-octane, and 1.0 mL of 1.5% sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0078] (2) Mix 1.5g triton, 0.5g n-hexanol, 2.5g n-octane, and 0.75M 1.0mL of CdCl 2 aqueous solution, or Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 30 minutes to prepare a uniform microemulsion 2.

[0079] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 3 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate and copper alginate nanospheres.

[0080]2. Preparation of immune probes and examples of their immune properties using cadmium alginate, lead alginate and copper alginate nanospheres:

[0081] 2.1 Tested sample: human serum

[0082] 2.2...

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PUM

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Abstract

The invention discloses cadmium alginate, lead alginate and copper alginate nanoparticles, a preparation method thereof and an application in preparation of electrochemical immune probes. The invention prepares nanoscale cadmium alginate, lead alginate and copper alginate spherical particles by respectively cross-linking sodium alginate with cadmium ions, lead ions or copper ions in microemulsion, and can generate distinguishable electrochemical signals. The preparation method of the invention is characterized by mild preparation conditions and can be directly used for labeling without adding signal substances. The electrochemical immunosensor prepared by using the cadmium alginate, lead alginate and copper alginate nanoparticles to label different antibodies can realize the simultaneous detection of three targets without partitioning. The cadmium alginate, lead alginate and copper alginate nano particle immunoprobe and the electrochemical immunosensor prepared therefrom will have broad application prospects in the field of immunosensing.

Description

technical field [0001] The invention relates to a preparation method of cadmium alginate, lead alginate and copper alginate nanoparticles and their application in preparing immune probes, belonging to the field of electrochemical sensors, which can be used for immune analysis and detection. Background technique [0002] Cancer is a general term for a class of malignant tumors. In my country, 3 million people are diagnosed with cancer every year, and this number is still increasing year by year. Cancer treatment is difficult and has a high mortality rate, which is one of the problems that plague human beings. Studies have shown that early detection and early treatment can effectively improve the cure rate of cancer and reduce mortality. Early screening of cancer is mainly aimed at tumor markers. Tumor markers are usually directly produced by embryonic tissue and tumor tissue, and its concentration changes can reflect the dynamic changes of the tumor, and are related to tum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/14C08L5/04G01N33/543G01N33/574
Inventor 马占芳王子凤
Owner CAPITAL NORMAL UNIVERSITY
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