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Method for efficient synthesis of difructose anhydride III

A double fructose anhydride, high-efficiency technology, applied in the field of functional food bioprocessing, can solve the problems of uneconomical and high prices, and achieve the effects of low production cost, low energy consumption and simple process

Active Publication Date: 2016-04-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method is to directly convert plant inulin to obtain double fructose III. First of all, plant inulin is needed. Compared with sucrose, inulin is relatively expensive, so it is not economical.

Method used

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  • Method for efficient synthesis of difructose anhydride III
  • Method for efficient synthesis of difructose anhydride III
  • Method for efficient synthesis of difructose anhydride III

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Dissolve the sucrose substrate completely with deionized water, control its concentration to 400g / L, adjust the pH value to 5.5, control the temperature at 20°C, add inulin sucrase at an amount of 10U / g sucrose, and react at a constant temperature for 30 minutes;

[0032] Raise the temperature of the reaction system to 55°C, and then add inulin fructotransferase to it, the concentration of the added inulin fructotransferase is 5U / g, the pH value is still controlled at 5.5, and the reaction is carried out at constant temperature for 12 hours;

[0033] The solution after the reaction was taken and centrifuged, the supernatant was filtered with a microporous membrane (0.22), and the filtrate was analyzed by HPLC equipped with a differential refraction display. HPLC conditions are: Sugarpak1, 6.5mmid×300mm calcium type cation exchange column, pure water as mobile phase, column temperature is 85°C, and tassel is 0.4ml / min. The injection volume was 10ul, and the concentration...

Embodiment 2

[0035] Dissolve the sucrose substrate completely with deionized water, control the concentration to 300g / L, adjust the pH value to 5.0, control the temperature at 22°C, add inulin sucrase at an amount of 15U / g sucrose, and react at a constant temperature for 60 minutes;

[0036] Raise the temperature of the reaction system to 60°C, and then add inulin fructosyltransferase to it, the concentration of the added inulin fructosyltransferase is 10U / g, the pH value is still controlled at 5.0, and the constant temperature reaction is 6h; the obtained double The conversion rate of fructose III reached 48%.

Embodiment 3

[0038] Dissolve the sucrose substrate completely with deionized water, control the concentration to 500g / L, adjust the pH value to 6.0, control the temperature at 30°C, add inulin sucrase, the addition amount is 5U / g sucrose, and react at constant temperature for 60min;

[0039] Raise the temperature of the reaction system to 50°C, and then add inulin fructosyltransferase to it, the concentration of the added inulin fructosyltransferase is 20U / g, the pH value is still controlled at 6.0, and the constant temperature reaction is 20h; the obtained double The conversion rate of fructose III reached 43%.

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Abstract

The invention discloses a method for efficient synthesis of difructose anhydride III. According to the method, saccharose is converted into inulin firstly by means of inulin sucrose without glycan separation, and then inulin is converted by means of inulin fructotransferase to generate functional disaccharide difructose anhydride III through synthesis. The technology is simple, efficiency is high, and the conversion rate of inulin for synthesis of difructose anhydride III can reach 40-54%. To obtain high-purity difructose anhydride III, micromolecule monosaccharide in reaction liquid is removed by means of yeast, and the finally obtained high-purity difructose anhydride III is easy to separate and purify and has broad market prospects.

Description

technical field [0001] The invention relates to a method for efficiently synthesizing difructose anhydride III, in particular to realize the efficient production of difructose anhydride III (Difructosanhydride, DFAIII) by a two-step method with two enzymes, and belongs to the technical field of functional food bioprocessing. Background technique [0002] In recent years, due to the improvement of living standards, diabetes, obesity and cardiovascular diseases have increased year by year and younger ages have appeared. Therefore, the development of sweeteners with low energy and multifunctional nutritional properties has become a focus of consumers' attention. [0003] Difructose III is a new type of functional sweetener, its structure is mainly formed by removing two water molecules from two fructose molecules. Since the hydroxyl groups on the anomeric carbon all form glycosidic bonds, bifructose III is a new type of non-reducing disaccharide. Due to the existence of such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/14C12P19/12
CPCC12P19/12C12N9/1051C12N9/88C12P19/14C12P19/18C12Y204/01009C12Y402/02017C12Y402/02018
Inventor 沭万孟江波郁书怀朱莺莺张涛
Owner JIANGNAN UNIV
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