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Method for promoting enzymatic hydrolysis of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, which is applied in the field of promoting lignocellulosic enzymatic hydrolysis, can solve the problems of high price and achieve the effect of avoiding desorption

Inactive Publication Date: 2016-04-27
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used non-ionic surfactants to promote enzymatic hydrolysis of lignocellulose after pretreatment are PEG and Tween series, but these non-ionic surfactants are often expensive and have an inhibitory effect on microorganisms in the subsequent fermentation production process

Method used

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  • Method for promoting enzymatic hydrolysis of lignocellulose
  • Method for promoting enzymatic hydrolysis of lignocellulose
  • Method for promoting enzymatic hydrolysis of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Metal Ion Screening

[0038] The wheat straw was pretreated with 0.5% dilute sulfuric acid at 140° C. for 1 hour, and then washed to neutrality, so as to obtain pretreated wheat straw (ie, pretreated lignocellulose). The above pretreated wheat straw was mixed with a sulfuric acid solution of pH 4.8 to obtain a liquid-solid mixture with a solid content of 5%. Add different metal salt solutions to the liquid-solid mixture so that the metal salt concentration in the liquid phase is 0.05-1g / L, and then treat it in a shaker at room temperature with a rotation speed of 150rpm for 1 hour, then filter to remove the liquid, and then use 15FPU / g The amount of cellulase used for solids, the enzymatic hydrolysis is carried out under the conditions of initial pH 4.8, 50° C., and 150 rpm. Among them, the temperature of the enzymatic hydrolysis system is raised from room temperature to 50°C using a gradient temperature rise method, that is, every 5°C increase and then heat preservati...

Embodiment 2

[0041] Effects of various metal ions on enzymatic hydrolysis of cellulose with different enzyme dosages

[0042] The used pretreated wheat straw raw material, metal ion treatment and enzymatic hydrolysis process temperature and pH parameters are the same as those in Example 1. Adopt 0.1g / L metal salt concentration, and the cellulase dosage of 5-40FPU / g solid. The control group did not add metal ions, and the enzymatic conversion rate of cellulose after 120 hours is shown in Table 2.

[0043] Table 2 Effects of different metal ions on the conversion rate of cellulose after 120 h at different cellulase dosages

[0044]

[0045] It can be seen from the above table that metal ions have a certain promotion effect on the conversion rate of cellulose under different cellulase dosages, while at lower cellulase dosages, metal ions, especially Mg 2+ showed the most significant promotion effect.

Embodiment 3

[0047] The effect of pH

[0048] The pretreated wheat straw raw material and the enzymatic hydrolysis process are the same as in Example 1. Using 0.1g / LMgCl 2 Concentration, changing MgCl 2 The pH value of the system when the solution is used to treat the straw after dilute acid pretreatment, and the treated solid is enzymatically hydrolyzed at the corresponding pH with a cellulase dosage of 5 FPU / g solid. Taking the pretreated wheat straw without adding metal ions as the control, the enzymatic conversion rate of cellulose after 120 h is shown in Table 3.

[0049] Table 3 pH vs. MgCl 2 Effects of Dilute Acid Pretreatment on Enzymatic Hydrolysis of Wheat Straw

[0050]

[0051] It can be seen from the above table that the pH effect on Mg 2+ The promoting effect of metal ions has a significant effect, and the promoting effect of metal ions becomes insignificant when treated at lower or higher pH, and the optimal pH is around 5.0.

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Abstract

The invention discloses a method for promoting enzymatic hydrolysis of lignocellulose. The method comprises the following steps: (1) mixing lignocellulose and a pH regulation solution for obtaining a first liquid-solid mixture, wherein the lignocellulose is subjected to pretreatment in advance, and the pH value of the pH regulation solution is 4.0-10.0; (2) adding a metal salt solution to the first liquid-solid mixture, and reacting for preset time to obtain a second liquid-solid mixture; (3) separating the second liquid-solid mixture to obtain a solid; and (4) enabling the solid to be subjected to enzymatic hydrolysis by utilizing cellulose. With the adoption of the method, the invalid adsorption of lignin for the cellulose can be effectively reduced, the enzymatic hydrolysis of the lignocellulose is promoted, and the saccharifying efficiency of the cellulose is improved; and the method is low in cost, and easy for popularization and use.

Description

technical field [0001] The invention relates to the field of biomass chemical industry, in particular to a method for promoting lignocellulosic enzyme-catalyzed hydrolysis. Background technique [0002] Lignocellulose is one of the most abundant biomass in nature. More than 40 million tons of lignocellulosic raw materials are produced in the world every year, accounting for 50% of the total global biomass, including agricultural and forestry waste, waste paper, energy crops, etc. , most of which are discarded without being used. The carbohydrate content of lignocellulosic composition is as high as 75%, which can serve as an important source of fermentable sugars for the production of liquid fuels and other chemicals. In recent years, with the continuous consumption of fossil energy and the increasingly prominent environmental problems, the development of biomass conversion to produce energy and chemicals has attracted widespread attention from all walks of life around the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/14C12P19/04
Inventor 赵雪冰陈可刘德华
Owner TSINGHUA UNIV
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