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Method for performing cyclic enzymolysis saccharification on lignocellulose by using multi-enzyme synergistic surfactant

A technology of surfactant and circulating enzyme, applied in fermentation and other directions, can solve the problems of high cost of enzyme, high cost and high amount of enzyme, and achieve the effect of simple operation, reducing the cost of enzymolysis and increasing the contact area.

Active Publication Date: 2022-04-12
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the addition of surfactants and the synergistic effect of multiple enzymes can increase the yield of enzymatic hydrolysis, the problem of high enzyme cost is still unsolved
At present, there is a problem of high cost in enzymatic saccharification of lignocellulose, which is mainly due to the high dosage of enzymes

Method used

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  • Method for performing cyclic enzymolysis saccharification on lignocellulose by using multi-enzyme synergistic surfactant
  • Method for performing cyclic enzymolysis saccharification on lignocellulose by using multi-enzyme synergistic surfactant

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the pretreatment of wood

[0027] (1) Clean poplar wood and use a pulverizer to pulverize it. After passing through an 80-mesh sieve, put the sawdust in an oven at 60° C. and bake to constant weight;

[0028] (2) Take 50g of sawdust, use 4% sodium hydroxide to mix well according to the ratio of material to liquid 1:10 (g:mL), and place it at 121°C for 90min;

[0029] (3) Wash the poplar sawdust with pure water until it is neutral, then put it in an oven at 60°C, bake it to constant weight, and set it aside.

Embodiment 2

[0030] Embodiment 2: the secondary pretreatment of hydrogen peroxide and laccase

[0031] (1) The sawdust treated in Example 1 was fully mixed with acetic acid sodium acetate buffer according to the ratio of solid to liquid at 1:10 (g:mL);

[0032] (2) Add 50 mL of 10% hydrogen peroxide and 50 U / g sawdust laccase, and pretreat at 50°C for 12 hours;

[0033] (3) Use a vacuum pump to filter the wood chips that have been pretreated twice to obtain filtrate and filter residue, wherein the filtrate is recovered and stored in cold storage for easy reuse, and the filter residue is ready for use.

Embodiment 3

[0034] Embodiment 3: the pre-enzymolysis of xylanase and surfactant

[0035] (1) the poplar filter residue obtained in Example 2 is thoroughly mixed with acetic acid sodium acetate buffer solution according to the ratio of solid to liquid 1:10 (g:mL);

[0036] (2) Add 60 U / g wood chips xylanase and 0.05 g / g wood chips non-ionic surfactant Tween-80 for pre-enzymolysis for 2 hours;

[0037] (3) Use a vacuum pump to filter the pre-enzymolyzed sawdust to obtain filtrate and filter residue, wherein the filtrate is recovered and stored in cold storage for easy reuse, and the filter residue is used for later use.

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Abstract

The invention discloses a method for saccharifying lignocellulose by multi-enzyme synergistic surfactant cyclic enzymolysis, poplar is saccharified mainly through four steps of pretreatment, secondary pretreatment, pre-enzymolysis and enzymolysis, and enzymatic hydrolysate is simply recovered for cyclic enzymolysis. According to the method, the poplar is pretreated mainly through sodium hydroxide, and the poplar subjected to alkali treatment is subjected to secondary pretreatment through laccase. And carrying out pre-enzymolysis on the poplar subjected to two-step pretreatment by using xylanase and a surfactant. And finally, carrying out enzymolysis saccharification on the poplar subjected to pre-enzymolysis by utilizing cellulase in cooperation with a surfactant. Wherein the laccase in the filtrate after the secondary pretreatment, the xylanase after the pre-enzymolysis, the cellulase after the enzymolysis and the surfactant are subjected to suction filtration and are recycled, so that the purpose of reducing the cost is achieved.

Description

technical field [0001] The invention relates to the field of biotransformation, in particular to a method for step-by-step pretreatment and multi-enzyme synergistic surfactant cycle enzymatic hydrolysis of saccharified poplar wood. Background technique: [0002] Lignocellulosic biomass exists in large quantities in nature, and is the most abundant and cheapest renewable resource that meets the requirements of sustainable development on the earth. However, due to the entanglement of cellulose, hemicellulose and lignin contained in lignocellulose to form a dense three-dimensional network space structure, the enzymatic hydrolysis efficiency is low. Lignin forms an amorphous, nonlinear and heterogeneous stereopolymeric structure, and hemicellulose runs through between cellulose and lignin, limiting the accessibility of catalytic factors for lignocellulose degradation. Therefore, during the conversion of lignocellulose, pretreatment must be carried out before enzymatic hydrolysi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/00
Inventor 曾柏全孙彦彦
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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