Deep calcium ion concentration monitoring method using photoacoustic-fluorescent complementary principle and device using the same

Abstract

The invention discloses a deep calcium ion concentration monitoring method using a photoacoustic-fluorescent complementary principle and a device using the same. The method can detect calcium ion concentration change through detection of change of intensity of a photoacoustic signal of a calcium ion fluorescent probe. The device comprises a photoacoustic-fluorescent signal excitation source, a bowl-shaped hollow focusing ultrasonic detector, a photomultiplier, a reflector, a light splitter, an expanded beam lens group, a microobjective, an ultrasonic coupling beaker, a sample bench, a 3D scanning platform, an optical filter, a signal amplifier, a two-channel parallel acquisition card and a computer with image reconstruction software, acquisition control software and motor control software. The method and device realize deep tissue calcium ion concentration monitoring, are convenient for operation, has calcium ion fluorescent probe sensitivity of 0.15 micromoles per liter and realizes monitoring of change of a deep tissue calcium ion concentration. The method and device can realize detection of change of a deep tissue calcium ion concentration in real time and through the rate of the change, a pathological change of tissues can be deduced.

Description

technical field [0001] The invention belongs to the technical field of non-destructive testing and measurement, and in particular relates to a method and device for monitoring calcium ion concentration in deep layers using the photoacoustic-fluorescence complementary principle. Background technique [0002] At present, the change of calcium ion concentration is mainly monitored by observing the fluorescent signal of the calcium ion fluorescent probe. However, due to the influence of biological tissue on fluorescence scattering, fluorescence imaging methods cannot monitor changes in calcium ion concentration in deep layers. However, photoacoustic signals (photo-induced ultrasound) are much less affected by tissue scattering than photons, so we combine the non-destructive photoacoustic imaging technology with the high sensitivity of pure optical imaging and the deep penetration characteristics of pure ultrasonic imaging. It overcomes the limitation of light scattering and rea...

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Problems solved by technology

However, due to the influence of biological tissues on fluorescence scattering, fluorescence imaging methods cannot monitor changes in calcium ion concentrations in deep layers.

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