A kind of fluorescence detection system and detection method of heavy metal cadmium
A fluorescence detection and heavy metal technology, applied in the field of heavy metal detection, can solve the problems of high equipment and operation costs, low detection limit of graphite furnace method, and needs further research, and achieve strong environmental adaptability, facilitate real-time monitoring, and excellent cadmium ion specific effect
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Embodiment 1
[0046] This example provides a detection system that can specifically recognize heavy metal cadmium, including nucleic acid aptamer DNA sequence, nucleic acid aptamer complementary DNA sequence and fluorescent dye Picogreen, and the rest is a buffer;
[0047] The nucleic acid aptamer DNA sequence is: 5'-ACCGACCGTGCTGGACTCTGGACTGTTGTGGTATTATTTTTGGTTGTGCAGTATGAGCGAGCGTTGCG-3';
[0048] The complementary DNA sequence of the nucleic acid aptamer is: 5'-CGCAACGCTCGCTCATACTGCACAACCAAAAATAATACCACAACAGTCCAGAGTCCAGCACGGTCGGT-3'.
Embodiment 2
[0050] The present embodiment utilizes the detection system provided in Example 1, and the agricultural irrigation water sample in a certain area in the suburbs of Beijing is the sample to be tested (the Cd content of the sample to be tested is determined by graphite furnace atomic absorption method, but not detected), according to the following Steps to detect:
[0051] (1) Take 7 parts of aptamer DNA with a volume of 50uL and a concentration of 1 μmol / L, and add them to the aptamer DNA with a volume of 100 μL and a concentration of 2ng / mL, 20ng / mL, 200ng / mL, 2μg / mL, 20μg / mL, and 200μg / mL cadmium ion standard solution and a control solution with a concentration of 0 ng / mL; after mixing and incubating for 20 min, the complementary strand DNA of the aptamer with a volume of 50 uL and a concentration of 1 μmol / L was added respectively (at this time, cadmium was used as the final target Concentrations from low to high are 0ng / mL, 1ng / mL, 10ng / mL, 100ng / mL, 1μg / mL, 10μg / mL, 100μg / ...
experiment example
[0062] In order to study the specificity of the heavy metal Cd aptamer, this experiment example selects the heavy metal ion Cu 2+ 、Na + , Mg 2+ 、K + 、As + , Ca 2+ , Hg 2+ , Zn 2+ As a potential interfering substance, the aptamer was detected under the same conditions as in Example 2 to Cd and other heavy metal ions Cu 2+ 、Na + , Mg 2+ 、K + 、As + , Ca 2+ , Hg 2+ , Zn 2+ (Metal ion concentration gradient is 0ng / mL, 1ng / mL, 10ng / mL, 1ug / mL, 100ug / mL) Select specificity and sensitivity.
[0063] Compared with the blank control (that is, there is no target but only aptamer DNA solution), the fluorescence spectrum of the blank control has a significant decline at 530nm, while Cu 2+ 、Na + , Mg 2+ 、K + 、As + , Ca 2+ , Hg 2+ , Zn 2+The spectral line of the solution with ions as the target substance changes very little, and the fluorescence change rate of the target substance added with Cd is significantly higher than that of other heavy metal ions. The above result...
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