Construction and application of a coaxial vascularized tissue engineered bone

A tissue-engineered bone and vascularization technology, used in tissue regeneration, femur, bone implants, etc., can solve problems such as failure of large-volume tissue-engineered bone construction, failure to achieve early vascularization in the axial area, etc., to promote osteogenesis. effect of effect

Inactive Publication Date: 2018-08-21
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a coaxial vascularized tissue engineered bone for the problem that the axial center area cannot achieve early vascularization in the current process of tissue engineered bone construction, which leads to the failure of large volume tissue engineered bone construction. construction plan

Method used

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  • Construction and application of a coaxial vascularized tissue engineered bone
  • Construction and application of a coaxial vascularized tissue engineered bone
  • Construction and application of a coaxial vascularized tissue engineered bone

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Experimental study on in vivo rapid formation of vascular network in hydrogel loaded with vascularized seed cells

[0032] (1) Acquisition of seed cells:

[0033]1) Acquisition of bone marrow endothelial progenitor cells (EPCs): extract 5ml of bone marrow, obtain mononuclear cells in the bone marrow by gradient centrifugation, culture in endothelial cell culture medium, change the medium every 2-3 days, and cultivate for 2-4 weeks Finally, through morphological observation and flow cytometry detection to identify the cell properties, after counting, they were inoculated into culture flasks for subculture, and the 3rd generation cells were taken for future use (see figure 2 );

[0034] 2) Acquisition of bone marrow mesenchymal stem cells (BMSCs): According to the bone marrow culture method, extract red bone marrow, anticoagulate with 500u / mL heparin solution, mix well, centrifuge at 1000r / min for 10min, discard the supernatant, add DMEM complete medium, reco...

Embodiment 2

[0041] Example 2: Experimental study of coaxial tissue engineered bone repairing load-bearing bone defects

[0042] (1) Acquisition of seed cells:

[0043] 1) Acquisition of bone marrow endothelial progenitor cells (EPCs): extract 5ml of bone marrow, obtain mononuclear cells in the bone marrow by gradient centrifugation, culture in endothelial cell culture medium, change the medium every 2-3 days, and cultivate for 2-4 weeks Afterwards, through morphological observation and flow cytometry detection to identify the nature of the cells, after counting, they were inoculated into culture flasks for subculture, and the 3rd generation cells were taken for later use;

[0044] 2) Acquisition of bone marrow mesenchymal stem cells (BMSCs): According to the bone marrow culture method, extract red bone marrow, anticoagulate with 500u / mL heparin solution, mix well, centrifuge at 1000r / min for 10min, discard the supernatant, add DMEM complete medium, record After a few days, they were inoc...

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Abstract

The invention relates to the technical field of tissue-engineered bones, and especially relates to a construction method and applications of coaxial type vascularized tissue-engineered bone for bone defect repairing. The construction method comprises (1) construction of ossified support in peripheral area; (2) construction of three dimensional blood vessel network in core area; (3) assembly of coaxial type tissue-engineered bones. The constructed coaxial type vascularized tissue-engineered bone can be used as a bone graft for repairing large section weight bearing bone defect, and the repairing effect is good in animal tests. At present, the construction of large volume tissue-engineered bones is still challenging, because the core area of tissue-engineered bone cannot be fully vascularized in the early stage; and the provided construction method can well solve the problem mentioned above so as to construct the vascularized tissue-engineered bone.

Description

technical field [0001] The invention relates to the technical field of tissue engineering bone, in particular to a construction method and application of coaxial vascularized tissue engineering bone for bone defect repair. Background technique [0002] Large-volume bone defects caused by various reasons such as trauma, infection, and tumor resection have always been a difficult point in clinical treatment, and bone transplantation provides the possibility to solve this problem. At present, autologous bone is still the first choice of bone graft source, but its supply is limited, and it is difficult to meet the clinical needs when it is applied. Therefore, finding a suitable bone graft substitute has become the primary problem in the research and repair of large-volume bone defects. [0003] Tissue-engineered bone technology has successfully constructed small-volume tissue-engineered bone with good osteogenic activity with the help of seed cells, growth factors, and scaffold...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/52A61L27/12A61F2/28
CPCA61F2/2846A61F2002/2817A61F2002/2825A61F2002/2835A61F2310/00293A61F2310/00359A61L27/12A61L27/3808A61L27/3834A61L27/52A61L2430/02
Inventor 王簕林启旺娄爱菊郭元杨逸禧何可人蔡梓红杨波金丹
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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