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Production method of high-efficiency keratinase

A technology of keratinase and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of polluting the environment, large energy consumption, waste of protein resources, etc.

Inactive Publication Date: 2016-05-11
SHENZHEN XIANKANGDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thermal degradation and other methods, the former has the problem of polluting the environment, the latter consumes a lot of energy, and can destroy some amino acids, reducing the nutritional value of the product
Other common keratin is animal hair, such as cow hair, wool, and human hair. Some of these keratin proteins are processed as commercial raw materials, and the rest are mostly treated as waste, which also causes environmental pollution and waste of protein resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Inoculate Leuconostoc enterocoliticus on the slant medium by coating or streaking, and rejuvenate at 18°C ​​for 18 hours to obtain the rejuvenated thalline; the composition of each 1000ml slant medium is: salt 6g , 5g of yeast powder, 5g of peptone, 10g of agar powder, 5g of taurine, 1g of microcrystalline cellulose, and 975g of water;

[0021] 2) Inoculate the rejuvenated bacteria into the liquid seed medium, and cultivate it for 18 hours at 18° C. under the condition of 100 rpm to obtain fermented seeds; the ingredients of each 1000 ml of the seed medium are: 3 g of salt, 5 g of yeast powder, and 5 g of peptone , agar powder 10g, Yizhiren 2g, wax gourd powder 1g, konjac powder 1g, water 981g;

[0022] 3) Put the fermented seeds into the fermenter according to 5% of the volume of the fermentation medium, the tank temperature is 18°C, and the tank pressure is 0.2kg / cm 2 , the stirring rate is 100rpm, the ventilation rate is 1:0.3, the fermentation and cultivation ti...

Embodiment 2

[0024] 1) Inoculate Leuconostoc enterocoliticus on the slant medium by coating or streaking, and rejuvenate at 37°C for 36 hours to obtain rejuvenated thalline; the composition of each 1000ml slant medium is: 14g of salt , Yeast powder 10g, peptone 15g, agar powder 19g, taurine 12g, sodium dodecyl sulfonate 2g, sodium benzoate 1g, microcrystalline cellulose 2g, bentonite 0.5g, water 950g;

[0025] 2) inoculate the rejuvenated bacteria into the liquid seed culture medium, and cultivate it for 36 hours at 37°C and 300rpm to obtain fermented seeds; the ingredients of each 1000ml seed culture medium are: salt 8g, yeast powder 10g, peptone 15g , agar powder 19g, Yizhiren 3g, wax gourd powder 2g, konjac powder 3g, water 956g;

[0026] 3) Put the fermented seeds into the fermenter according to 15% of the volume of the fermentation medium, the tank temperature is 37°C, and the tank pressure is 0.8kg / cm 2 , stirring rate 300rpm, air flow is 1: 2.0, fermentation time 60 hours, obtains ...

Embodiment 3

[0028] 1) Inoculate Leuconostoc enterica enterica on the slant medium by coating or streaking, and rejuvenate at 28°C for 27 hours to obtain the rejuvenated thalline; the composition of each 1000ml slant medium is: 10g of salt , 7.5g of yeast powder, 10g of peptone, 15g of agar powder, 9g of taurine, 1.5g of microcrystalline cellulose, and 962g of water;

[0029] 2) Inoculate the rejuvenated thallus into the liquid seed medium, and cultivate it for 27 hours at 28° C. under the condition of 200 rpm to obtain the fermented seeds; the ingredients of each 1000 ml of the seed medium are: 5.5 g of salt, 7.5 g of yeast powder, 10g of peptone, 14.5g of agar powder, 2.5g of Yizhiren, 1.5g of winter melon powder, 2g of konjac powder, and the balance is 968g of water;

[0030] 3) Put the fermented seeds into the fermenter according to 10% of the volume of the fermentation medium, the tank temperature is 28°C, and the tank pressure is 0.5kg / cm 2 , the stirring rate is 200rpm, the ventila...

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Abstract

The invention discloses a production method of high-efficiency keratinase. The method comprises the following steps: inoculating Leuconostoc mesenteroides into a slant culture medium by a coating or scribing process, and carrying out rejuvenation culture at 18-37 DEG C for 18-36 hours to obtain rejuvenated cells; inoculating the rejuvenated cells into a liquid seed culture medium, and culturing at 18-37 DEG C under the condition of 100-300 rpm for 18-36 hours to obtain a fermentation seed; and inoculating the fermentation seed, which accounts for 5-15 vol% of the fermentation culture medium, into a fermentation tank, and fermenting and culturing for 18-60 hours to obtain the high-efficiency keratinase, wherein the tank temperature is 18-37 DEG C, the tank pressure is 0.2-0.8 kg / cm<2>, the stirring rate is 100-300 rpm and the ventilatory capacity is 1:(0.3-2.0). The prepared keratinase is widely used in the fields of tanning unhairing and keratin hydrolysis.

Description

technical field [0001] The invention relates to a method for producing keratinase, in particular to a highly efficient method for producing keratinase. Background technique [0002] Keratinase is an enzyme that can specifically degrade keratin, which is produced by various microorganisms such as bacteria, actinomycetes and fungi. As a by-product of poultry breeding and slaughtering industry, feathers have a huge annual output and are rich in protein and amino acids, so they are potential excellent protein resources. Reasonable utilization of feathers can reduce the pollution of waste to the environment on the one hand, and can be used as a feed protein for livestock and poultry feeding. In the traditional process of using feathers, acid-base hydrolysis is mainly used. Thermal degradation and other methods, the former has the problem of polluting the environment, the latter consumes a lot of energy, and can destroy some amino acids, reducing the nutritional value of the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/52C12R1/01
CPCC12N9/52
Inventor 谢海涛董景峰谢来宾陈佩王鹏亭柴行周秋云
Owner SHENZHEN XIANKANGDA BIOTECH CO LTD
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