Kit and method for measuring content of free fatty acid

Abstract

The invention discloses a kit and method for measuring the content of free fatty acid. Reagents of the kit comprise the first reagent composed of isooctane, the second reagent formed by dissolving sodium acetate in distilled water, then adding pyridine and mixing the constituents uniformly and the third reagent formed by dissolving copper acetate in distilled water, then adding pyridine and mixing the constituents uniformly. The method comprises the steps that free fatty acid in a sample is extracted through the isooctane, then the free fatty acid reacts with copper salt to generate copper soap under the weak acidic condition, and the content of free fatty acid in the reaction sample at the light absorption value of 715 nm is measured. According to the method, based on measurement conducted through an existing colorimetric method, the extraction method and operation steps are improved, and the effects of simple extraction and fast, simple and convenient measurement are achieved. According to the method, the sample pre-treatment process is simple and convenient, and an extracting solution is singular in constituent and low in cost. According to the measurement steps of the method, only the three reagents are needed for completing measurement, and the process is simple, convenient and fast.

Description

technical field

[0001] The invention belongs to the field of life sciences and relates to a kit, in particular to a free fatty acid content determination kit and a method thereof. Background technique

[0002] Free fatty acids (FFA) are both products of fat hydrolysis and substrates for fat synthesis. The concentration of free fatty acids is related to lipid metabolism, glucose metabolism, and endocrine function, and can also reflect the quality changes in food storage.

[0003] At present, the existing methods for detecting the content of free fatty acids in the market are mainly gas chromatography, high performance liquid chromatography and colorimetry. Gas chromatography uses organic solvent mixed solution and sulfuric acid to treat the sample, and uses organic solvent to extract free fatty acids at high temperature, and then conducts gas chromatography analysis. High-performance liquid chromatography uses organic solvents to extract free fatty acids in samples, followe...